Abstract
Methods of femtosecond laser ablation in deionized water were used to fabricate ultrasmall (< 2 nm), bare (ligand-free) organic luminophore DCEtDCS nanoparticles, which exhibit aggregation enhanced emission in the green range (533 nm) with the quantum yield exceeding 58% and provide no concentration quenching. In contrast to chemically synthesized counterparts, laser-synthesized DCEtDCS nanoparticles do not contain any organic impurities due to their preparation in aqueous medium and do not require surfactants to stabilize colloidal solutions, which makes them highly suitable for intracellular uptake and bioimaging. The highly negative surface charge of these nanoparticles impeded their cellular uptake, but when the surface was coated with chitosan, a cationic polymer, intracellular uptake in microglia was achieved. Using in vitro model, we finally demonstrate the efficient employment of ultrasmall and surfactant free fluorescent organic nanoparticles prepared by laser ablation as markers in bioimaging.
Original language | English |
---|---|
Title of host publication | Synthesis and Photonics of Nanoscale Materials XVI |
Editors | Andrei V. Kabashin, David B. Geohegan, Jan J. Dubowski |
Publisher | SPIE |
ISBN (Electronic) | 9781510624566 |
DOIs | |
Publication status | Published - 2019 |
Event | Synthesis and Photonics of Nanoscale Materials XVI 2019 - San Francisco, United States Duration: 2019 Feb 2 → 2019 Feb 3 |
Publication series
Name | Proceedings of SPIE - The International Society for Optical Engineering |
---|---|
Volume | 10907 |
ISSN (Print) | 0277-786X |
ISSN (Electronic) | 1996-756X |
Conference
Conference | Synthesis and Photonics of Nanoscale Materials XVI 2019 |
---|---|
Country/Territory | United States |
City | San Francisco |
Period | 19/2/2 → 19/2/3 |
Bibliographical note
Funding Information:The support at Buffalo from the Air Force Office of Scientific Research (No. FA9550-18-1-0042) is acknowledged. The authors acknowledge the contribution from ITMO cancer AVIESAN (National Alliance for the Life Sciences & Health) within the framework of the Cancer Plan (GRAVITY Project). The authors also acknowledge support from the MEPhI Academic Excellence Project (Contract No. 02.a03.21.0005).
Funding Information:
The support at Buffalo from the Air Force Office of Scientific Research (No. FA9550-18-1-0042) is acknowledged. The authors acknowledge the contribution from ITMO cancer AVIESAN (National Alliance for the Life Sciences & Health) within the framework of the Cancer Plan (GRAVITY Project). The authors also acknowledge support from the MEPhI Academic Excellence Project (Contract No. 02.a03.21.0005)
Publisher Copyright:
Copyright © 2019 SPIE.
Keywords
- aggregation-induced enhanced emission luminophore dyes
- femtosecond laser ablation in water
- fluorescent organic nanoparticles
ASJC Scopus subject areas
- Electronic, Optical and Magnetic Materials
- Condensed Matter Physics
- Computer Science Applications
- Applied Mathematics
- Electrical and Electronic Engineering