Laser-ablative synthesis of aggregation-induced enhanced emission luminophore dyes in aqueous solutions

C. K. Lim; A. A. Popov; G. Tselikov; J. Heo; A. Pliss; S. Kim; A. V. Kabashin; P. N. Prasad

doi:10.1117/12.2513821

Laser-ablative synthesis of aggregation-induced enhanced emission luminophore dyes in aqueous solutions

C. K. Lim, A. A. Popov, G. Tselikov, J. Heo, A. Pliss, S. Kim, A. V. Kabashin, P. N. Prasad

KU-KIST Graduate School of Converging Science and Technology

Research output: Chapter in Book/Report/Conference proceeding › Conference contribution

Abstract

Methods of femtosecond laser ablation in deionized water were used to fabricate ultrasmall (< 2 nm), bare (ligand-free) organic luminophore DCEtDCS nanoparticles, which exhibit aggregation enhanced emission in the green range (533 nm) with the quantum yield exceeding 58% and provide no concentration quenching. In contrast to chemically synthesized counterparts, laser-synthesized DCEtDCS nanoparticles do not contain any organic impurities due to their preparation in aqueous medium and do not require surfactants to stabilize colloidal solutions, which makes them highly suitable for intracellular uptake and bioimaging. The highly negative surface charge of these nanoparticles impeded their cellular uptake, but when the surface was coated with chitosan, a cationic polymer, intracellular uptake in microglia was achieved. Using in vitro model, we finally demonstrate the efficient employment of ultrasmall and surfactant free fluorescent organic nanoparticles prepared by laser ablation as markers in bioimaging.

Original language	English
Title of host publication	Synthesis and Photonics of Nanoscale Materials XVI
Editors	Andrei V. Kabashin, David B. Geohegan, Jan J. Dubowski
Publisher	SPIE
ISBN (Electronic)	9781510624566
DOIs	https://doi.org/10.1117/12.2513821
Publication status	Published - 2019
Event	Synthesis and Photonics of Nanoscale Materials XVI 2019 - San Francisco, United States Duration: 2019 Feb 2 → 2019 Feb 3

Publication series

Name	Proceedings of SPIE - The International Society for Optical Engineering
Volume	10907
ISSN (Print)	0277-786X
ISSN (Electronic)	1996-756X

Conference

Conference	Synthesis and Photonics of Nanoscale Materials XVI 2019
Country/Territory	United States
City	San Francisco
Period	19/2/2 → 19/2/3

Bibliographical note

Funding Information:
The support at Buffalo from the Air Force Office of Scientific Research (No. FA9550-18-1-0042) is acknowledged. The authors acknowledge the contribution from ITMO cancer AVIESAN (National Alliance for the Life Sciences & Health) within the framework of the Cancer Plan (GRAVITY Project). The authors also acknowledge support from the MEPhI Academic Excellence Project (Contract No. 02.a03.21.0005).

Funding Information:
The support at Buffalo from the Air Force Office of Scientific Research (No. FA9550-18-1-0042) is acknowledged. The authors acknowledge the contribution from ITMO cancer AVIESAN (National Alliance for the Life Sciences & Health) within the framework of the Cancer Plan (GRAVITY Project). The authors also acknowledge support from the MEPhI Academic Excellence Project (Contract No. 02.a03.21.0005)

Publisher Copyright:
Copyright © 2019 SPIE.

Keywords

aggregation-induced enhanced emission luminophore dyes
femtosecond laser ablation in water
fluorescent organic nanoparticles

ASJC Scopus subject areas

Electronic, Optical and Magnetic Materials
Condensed Matter Physics
Computer Science Applications
Applied Mathematics
Electrical and Electronic Engineering

Access to Document

10.1117/12.2513821

Cite this

Lim, C. K., Popov, A. A., Tselikov, G., Heo, J., Pliss, A., Kim, S., Kabashin, A. V., & Prasad, P. N. (2019). Laser-ablative synthesis of aggregation-induced enhanced emission luminophore dyes in aqueous solutions. In A. V. Kabashin, D. B. Geohegan, & J. J. Dubowski (Eds.), Synthesis and Photonics of Nanoscale Materials XVI Article 109070U (Proceedings of SPIE - The International Society for Optical Engineering; Vol. 10907). SPIE. https://doi.org/10.1117/12.2513821

Laser-ablative synthesis of aggregation-induced enhanced emission luminophore dyes in aqueous solutions. / Lim, C. K.; Popov, A. A.; Tselikov, G. et al.
Synthesis and Photonics of Nanoscale Materials XVI. ed. / Andrei V. Kabashin; David B. Geohegan; Jan J. Dubowski. SPIE, 2019. 109070U (Proceedings of SPIE - The International Society for Optical Engineering; Vol. 10907).

Research output: Chapter in Book/Report/Conference proceeding › Conference contribution

Lim, CK, Popov, AA, Tselikov, G, Heo, J, Pliss, A, Kim, S, Kabashin, AV & Prasad, PN 2019, Laser-ablative synthesis of aggregation-induced enhanced emission luminophore dyes in aqueous solutions. in AV Kabashin, DB Geohegan & JJ Dubowski (eds), Synthesis and Photonics of Nanoscale Materials XVI., 109070U, Proceedings of SPIE - The International Society for Optical Engineering, vol. 10907, SPIE, Synthesis and Photonics of Nanoscale Materials XVI 2019, San Francisco, United States, 19/2/2. https://doi.org/10.1117/12.2513821

Lim CK, Popov AA, Tselikov G, Heo J, Pliss A, Kim S et al. Laser-ablative synthesis of aggregation-induced enhanced emission luminophore dyes in aqueous solutions. In Kabashin AV, Geohegan DB, Dubowski JJ, editors, Synthesis and Photonics of Nanoscale Materials XVI. SPIE. 2019. 109070U. (Proceedings of SPIE - The International Society for Optical Engineering). doi: 10.1117/12.2513821

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abstract = "Methods of femtosecond laser ablation in deionized water were used to fabricate ultrasmall (< 2 nm), bare (ligand-free) organic luminophore DCEtDCS nanoparticles, which exhibit aggregation enhanced emission in the green range (533 nm) with the quantum yield exceeding 58% and provide no concentration quenching. In contrast to chemically synthesized counterparts, laser-synthesized DCEtDCS nanoparticles do not contain any organic impurities due to their preparation in aqueous medium and do not require surfactants to stabilize colloidal solutions, which makes them highly suitable for intracellular uptake and bioimaging. The highly negative surface charge of these nanoparticles impeded their cellular uptake, but when the surface was coated with chitosan, a cationic polymer, intracellular uptake in microglia was achieved. Using in vitro model, we finally demonstrate the efficient employment of ultrasmall and surfactant free fluorescent organic nanoparticles prepared by laser ablation as markers in bioimaging.",

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note = "Funding Information: The support at Buffalo from the Air Force Office of Scientific Research (No. FA9550-18-1-0042) is acknowledged. The authors acknowledge the contribution from ITMO cancer AVIESAN (National Alliance for the Life Sciences & Health) within the framework of the Cancer Plan (GRAVITY Project). The authors also acknowledge support from the MEPhI Academic Excellence Project (Contract No. 02.a03.21.0005). Funding Information: The support at Buffalo from the Air Force Office of Scientific Research (No. FA9550-18-1-0042) is acknowledged. The authors acknowledge the contribution from ITMO cancer AVIESAN (National Alliance for the Life Sciences & Health) within the framework of the Cancer Plan (GRAVITY Project). The authors also acknowledge support from the MEPhI Academic Excellence Project (Contract No. 02.a03.21.0005) Publisher Copyright: Copyright {\textcopyright} 2019 SPIE.; Synthesis and Photonics of Nanoscale Materials XVI 2019 ; Conference date: 02-02-2019 Through 03-02-2019",

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Laser-ablative synthesis of aggregation-induced enhanced emission luminophore dyes in aqueous solutions

Abstract

Publication series

Conference

Bibliographical note

Keywords

ASJC Scopus subject areas

Access to Document

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