Laser-ablative synthesis of aggregation-induced enhanced emission luminophore dyes in aqueous solutions

C. K. Lim, A. A. Popov, G. Tselikov, J. Heo, A. Pliss, S. Kim, A. V. Kabashin, P. N. Prasad

Research output: Chapter in Book/Report/Conference proceedingConference contribution


Methods of femtosecond laser ablation in deionized water were used to fabricate ultrasmall (< 2 nm), bare (ligand-free) organic luminophore DCEtDCS nanoparticles, which exhibit aggregation enhanced emission in the green range (533 nm) with the quantum yield exceeding 58% and provide no concentration quenching. In contrast to chemically synthesized counterparts, laser-synthesized DCEtDCS nanoparticles do not contain any organic impurities due to their preparation in aqueous medium and do not require surfactants to stabilize colloidal solutions, which makes them highly suitable for intracellular uptake and bioimaging. The highly negative surface charge of these nanoparticles impeded their cellular uptake, but when the surface was coated with chitosan, a cationic polymer, intracellular uptake in microglia was achieved. Using in vitro model, we finally demonstrate the efficient employment of ultrasmall and surfactant free fluorescent organic nanoparticles prepared by laser ablation as markers in bioimaging.

Original languageEnglish
Title of host publicationSynthesis and Photonics of Nanoscale Materials XVI
EditorsAndrei V. Kabashin, David B. Geohegan, Jan J. Dubowski
ISBN (Electronic)9781510624566
Publication statusPublished - 2019
EventSynthesis and Photonics of Nanoscale Materials XVI 2019 - San Francisco, United States
Duration: 2019 Feb 22019 Feb 3

Publication series

NameProceedings of SPIE - The International Society for Optical Engineering
ISSN (Print)0277-786X
ISSN (Electronic)1996-756X


ConferenceSynthesis and Photonics of Nanoscale Materials XVI 2019
Country/TerritoryUnited States
CitySan Francisco


  • aggregation-induced enhanced emission luminophore dyes
  • femtosecond laser ablation in water
  • fluorescent organic nanoparticles

ASJC Scopus subject areas

  • Electronic, Optical and Magnetic Materials
  • Condensed Matter Physics
  • Computer Science Applications
  • Applied Mathematics
  • Electrical and Electronic Engineering


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