Laser-ablative synthesis of aggregation-induced enhanced emission luminophore dyes in aqueous solutions

C. K. Lim, A. A. Popov, G. Tselikov, J. Heo, A. Pliss, S. Kim, A. V. Kabashin, P. N. Prasad

    Research output: Chapter in Book/Report/Conference proceedingConference contribution

    Abstract

    Methods of femtosecond laser ablation in deionized water were used to fabricate ultrasmall (< 2 nm), bare (ligand-free) organic luminophore DCEtDCS nanoparticles, which exhibit aggregation enhanced emission in the green range (533 nm) with the quantum yield exceeding 58% and provide no concentration quenching. In contrast to chemically synthesized counterparts, laser-synthesized DCEtDCS nanoparticles do not contain any organic impurities due to their preparation in aqueous medium and do not require surfactants to stabilize colloidal solutions, which makes them highly suitable for intracellular uptake and bioimaging. The highly negative surface charge of these nanoparticles impeded their cellular uptake, but when the surface was coated with chitosan, a cationic polymer, intracellular uptake in microglia was achieved. Using in vitro model, we finally demonstrate the efficient employment of ultrasmall and surfactant free fluorescent organic nanoparticles prepared by laser ablation as markers in bioimaging.

    Original languageEnglish
    Title of host publicationSynthesis and Photonics of Nanoscale Materials XVI
    EditorsAndrei V. Kabashin, David B. Geohegan, Jan J. Dubowski
    PublisherSPIE
    ISBN (Electronic)9781510624566
    DOIs
    Publication statusPublished - 2019
    EventSynthesis and Photonics of Nanoscale Materials XVI 2019 - San Francisco, United States
    Duration: 2019 Feb 22019 Feb 3

    Publication series

    NameProceedings of SPIE - The International Society for Optical Engineering
    Volume10907
    ISSN (Print)0277-786X
    ISSN (Electronic)1996-756X

    Conference

    ConferenceSynthesis and Photonics of Nanoscale Materials XVI 2019
    Country/TerritoryUnited States
    CitySan Francisco
    Period19/2/219/2/3

    Bibliographical note

    Funding Information:
    The support at Buffalo from the Air Force Office of Scientific Research (No. FA9550-18-1-0042) is acknowledged. The authors acknowledge the contribution from ITMO cancer AVIESAN (National Alliance for the Life Sciences & Health) within the framework of the Cancer Plan (GRAVITY Project). The authors also acknowledge support from the MEPhI Academic Excellence Project (Contract No. 02.a03.21.0005).

    Funding Information:
    The support at Buffalo from the Air Force Office of Scientific Research (No. FA9550-18-1-0042) is acknowledged. The authors acknowledge the contribution from ITMO cancer AVIESAN (National Alliance for the Life Sciences & Health) within the framework of the Cancer Plan (GRAVITY Project). The authors also acknowledge support from the MEPhI Academic Excellence Project (Contract No. 02.a03.21.0005)

    Publisher Copyright:
    Copyright © 2019 SPIE.

    Keywords

    • aggregation-induced enhanced emission luminophore dyes
    • femtosecond laser ablation in water
    • fluorescent organic nanoparticles

    ASJC Scopus subject areas

    • Electronic, Optical and Magnetic Materials
    • Condensed Matter Physics
    • Computer Science Applications
    • Applied Mathematics
    • Electrical and Electronic Engineering

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