Laser Microsurgery in Caenorhabditis elegans

Christopher Fang-Yen; Christopher V. Gabel; Aravinthan D.T. Samuel; Cornelia I. Bargmann; Leon Avery

doi:10.1016/B978-0-12-394620-1.00006-0

Laser Microsurgery in Caenorhabditis elegans

Christopher Fang-Yen, Christopher V. Gabel, Aravinthan D.T. Samuel, Cornelia I. Bargmann, Leon Avery

Research output: Chapter in Book/Report/Conference proceeding › Chapter

92 Citations (Scopus)

Abstract

Laser killing of cell nuclei has long been a powerful means of examining the roles of individual cells in C. elegans. Advances in genetics, laser technology, and imaging have further expanded the capabilities and usefulness of laser surgery. Here, we review the implementation and application of currently used methods for target edoptical disruption in C. elegans.

Original language	English
Title of host publication	Methods in Cell Biology
Publisher	Academic Press Inc.
Pages	177-206
Number of pages	30
DOIs	https://doi.org/10.1016/B978-0-12-394620-1.00006-0
Publication status	Published - 2012

Publication series

Name	Methods in Cell Biology
Volume	107
ISSN (Print)	0091-679X

Bibliographical note

Funding Information:
We thank John Sulston and Ron Ellis for allowing us to include Figs. 2 and 4 and Fig. 3 , respectively, and Niels Ringstad for helpful comments. This work was supported by the National Science Foundation and National Institutes of Health.

Keywords

Ablation
Chemosensation
Laser killing
Microbeam
Microfluidics
Microsurgery

ASJC Scopus subject areas

Cell Biology

Access to Document

10.1016/B978-0-12-394620-1.00006-0

Cite this

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AU - Bargmann, Cornelia I.

AU - Avery, Leon

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AB - Laser killing of cell nuclei has long been a powerful means of examining the roles of individual cells in C. elegans. Advances in genetics, laser technology, and imaging have further expanded the capabilities and usefulness of laser surgery. Here, we review the implementation and application of currently used methods for target edoptical disruption in C. elegans.

KW - Ablation

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KW - Laser killing

KW - Microbeam

KW - Microfluidics

KW - Microsurgery

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Laser Microsurgery in Caenorhabditis elegans

Abstract

Publication series

Bibliographical note

Keywords

ASJC Scopus subject areas

Access to Document

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