Laser scanning reflection-matrix microscopy for aberration-free imaging through intact mouse skull

Seokchan Yoon, Hojun Lee, Jin Hee Hong, Yong Sik Lim, Wonshik Choi

Research output: Contribution to journalArticlepeer-review

33 Citations (Scopus)


A mouse skull is a barrier for high-resolution optical imaging because its thick and inhomogeneous internal structures induce complex aberrations varying drastically from position to position. Invasive procedures creating either thinned-skull or open-skull windows are often required for the microscopic imaging of brain tissues underneath. Here, we propose a label-free imaging modality termed laser scanning reflection-matrix microscopy for recording the amplitude and phase maps of reflected waves at non-confocal points as well as confocal points. The proposed method enables us to find and computationally correct up to 10,000 angular modes of aberrations varying at every 10 × 10 µm2 patch in the sample plane. We realized reflectance imaging of myelinated axons in vivo underneath an intact mouse skull, with an ideal diffraction-limited spatial resolution of 450 nm. Furthermore, we demonstrated through-skull two-photon fluorescence imaging of neuronal dendrites and their spines by physically correcting the aberrations identified from the reflection matrix.

Original languageEnglish
Article number5721
JournalNature communications
Issue number1
Publication statusPublished - 2020 Dec

Bibliographical note

Funding Information:
This work was supported by the Institute for Basic Science (IBS-R023-D1).

Publisher Copyright:
© 2020, The Author(s).

ASJC Scopus subject areas

  • General
  • General Physics and Astronomy
  • General Chemistry
  • General Biochemistry,Genetics and Molecular Biology


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