Leisingera thetidis sp. nov., isolated from coastal water

Min Ju Kim, Yong Guan, Hanna Choe, Zhun Li, Namhyun Chung, Yoon Ho Kang, Mi Kyung Lee

Research output: Contribution to journalArticlepeer-review

Abstract

A Gram-strain-negative, strictly aerobic, rod-shaped, catalase-positive, oxidase-positive and pinkish beige colony-forming bacterial strain designated as BMJM1T was isolated from a marine sample collected from coastal water near Tongyeong, Republic of Korea. The results of phylogenetic analysis based on 16S rRNA gene sequences indicated that BMJM1T represents a member of the genus Leisingera as it is closely related to Leisingera daeponensis KCTC 12794T (98.27%), Leisingera caerulea DSM 24564T (97.98%), Leisingera aquaemixtae KCTC 32538T (97.91%), Leisingera methylohalidivorans DSM 14336T (97.26%) and Leisingera aquimarina DSM 24565T (97.25%). Optimal growth occurred at 25–30°C, pH 7.0 and with 2% NaCl. Digital DNA–DNA hybridisation (dDDH) and average nucleotide identity (ANI) values between strain BMJM1T and the closely related species of the genus Leisingera were below 40 and 90%, respectively, which are far below the thresholds to delineate a novel species. The predominant fatty acids (>10%) are summed feature 8 (C18:1 ω7c and/or C18:1 ω6c) (68.4%) and C14:1 iso E (11.6%). The major polar lipids were phosphatidylethanolamine and phospholipid. The major isoprenoid quinone was ubiquinone-10. The DNA G+C content was 64.0%. On the basis of the results of the polyphasic taxonomic characterisation, BMJM1T represents a novel species of the genus Leisingera, for which the name is Leisingera thetidis sp. nov. is proposed, with that type strain BMJM1T (= KCTC 92110T = GDMCC 1.2992T).

Original languageEnglish
Article number005945
JournalInternational Journal of Systematic and Evolutionary Microbiology
Volume73
Issue number6
DOIs
Publication statusPublished - 2023 Jun 1

Bibliographical note

Funding Information:
This research was supported by the National Institute of Agricultural Sciences (PJ015298), National Institute of Environmental Research (NIER) funded by the Ministry of Environment (MOE) of the Republic of Korea (NIER-2021-01-01-044) and by the National Research Foundation of Korea (NRF) Grant funded by the Korea government (MSIT) (NRF-2020R1A2C2012111 and NRF-2021R1C1C1008377).

Publisher Copyright:
© 2023 The Authors.

Keywords

  • fatty acids
  • novel bacterium
  • polyphasic taxonomic characterization
  • taxonomy

ASJC Scopus subject areas

  • Microbiology
  • Ecology, Evolution, Behavior and Systematics

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