Leptin-induced matrix metalloproteinase-2 secretion is suppressed by trans-10,cis-12 conjugated linoleic acid

Hyun Seuk Moon; Hong Gu Lee; Ji Hye Seo; Chung Soo Chung; Ding Ding Guo; Tae Gyu Kim; Yun Jaie Choi; Chong Su Cho

doi:10.1016/j.bbrc.2007.03.068

Leptin-induced matrix metalloproteinase-2 secretion is suppressed by trans-10,cis-12 conjugated linoleic acid

Hyun Seuk Moon, Hong Gu Lee, Ji Hye Seo, Chung Soo Chung, Ding Ding Guo, Tae Gyu Kim, Yun Jaie Choi, Chong Su Cho

Research output: Contribution to journal › Article › peer-review

18 Citations (Scopus)

Abstract

It has long been recognized that leptin, a hormone made by adipocytes, is an important circulating signal for the regulation of body weight. In addition, matrix metalloproteinase (MMP), especially MMP-2, an adipocyte-secreted protein which promotes multi-cellular adipose clusters, is up-regulated in obesity. The present study is designed to evaluate whether trans-10,cis-12 conjugated linoleic acid (t-CLA) can suppress leptin-induced MMP-2 secretion in 3T3-L1 cells. The result showed that expressions of adipocyte marker proteins were significantly reduced by t-CLA-treated cultures, but not by linoleic acid (LA)-treated ones. Interestingly, MMP-2 secretion was significantly increased by leptin-treated cultures, thereby leading to accelerate adipocyte differentiation, indicating that MMP-2 was a necessary mediator of adipogenesis. However, increasing concentration of t-CLA significantly reduced leptin-induced MMP-2 secretion and triglyceride (TG) content. These findings provide support for a role for t-CLA in the regulation of metabolism in leptin-induced adipose tissue development.

Original language	English
Pages (from-to)	955-960
Number of pages	6
Journal	Biochemical and biophysical research communications
Volume	356
Issue number	4
DOIs	https://doi.org/10.1016/j.bbrc.2007.03.068
Publication status	Published - 2007 May 18

Bibliographical note

Funding Information:
This work was supported by the Agricultural R&D Promotion Center (ARPC) (2006-0053). We thank the National Instrumentation Center for Environmental Management (NICEM) and Research Institute of Pharmaceutical Sciences (RIPS) for measurement of PKC activation, ROS production, and NF-κB activation. We also thank Prof. Byung-Hoon Lee for technical assistance. H.S. Moon and J.H. Seo were supported by Brain Korea 21 (BK21) grant.

Keywords

Conjugated linoleic acid
Leptin
Lipid accumulation
Matrix metalloproteinase-2

ASJC Scopus subject areas

Biophysics
Biochemistry
Molecular Biology
Cell Biology

UN SDGs

This output contributes to the following UN Sustainable Development Goals (SDGs)

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10.1016/j.bbrc.2007.03.068

Cite this

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title = "Leptin-induced matrix metalloproteinase-2 secretion is suppressed by trans-10,cis-12 conjugated linoleic acid",

abstract = "It has long been recognized that leptin, a hormone made by adipocytes, is an important circulating signal for the regulation of body weight. In addition, matrix metalloproteinase (MMP), especially MMP-2, an adipocyte-secreted protein which promotes multi-cellular adipose clusters, is up-regulated in obesity. The present study is designed to evaluate whether trans-10,cis-12 conjugated linoleic acid (t-CLA) can suppress leptin-induced MMP-2 secretion in 3T3-L1 cells. The result showed that expressions of adipocyte marker proteins were significantly reduced by t-CLA-treated cultures, but not by linoleic acid (LA)-treated ones. Interestingly, MMP-2 secretion was significantly increased by leptin-treated cultures, thereby leading to accelerate adipocyte differentiation, indicating that MMP-2 was a necessary mediator of adipogenesis. However, increasing concentration of t-CLA significantly reduced leptin-induced MMP-2 secretion and triglyceride (TG) content. These findings provide support for a role for t-CLA in the regulation of metabolism in leptin-induced adipose tissue development.",

keywords = "Conjugated linoleic acid, Leptin, Lipid accumulation, Matrix metalloproteinase-2",

author = "Moon, {Hyun Seuk} and Lee, {Hong Gu} and Seo, {Ji Hye} and Chung, {Chung Soo} and Guo, {Ding Ding} and Kim, {Tae Gyu} and Choi, {Yun Jaie} and Cho, {Chong Su}",

note = "Funding Information: This work was supported by the Agricultural R&D Promotion Center (ARPC) (2006-0053). We thank the National Instrumentation Center for Environmental Management (NICEM) and Research Institute of Pharmaceutical Sciences (RIPS) for measurement of PKC activation, ROS production, and NF-κB activation. We also thank Prof. Byung-Hoon Lee for technical assistance. H.S. Moon and J.H. Seo were supported by Brain Korea 21 (BK21) grant.",

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doi = "10.1016/j.bbrc.2007.03.068",

language = "English",

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T1 - Leptin-induced matrix metalloproteinase-2 secretion is suppressed by trans-10,cis-12 conjugated linoleic acid

AU - Moon, Hyun Seuk

AU - Lee, Hong Gu

AU - Seo, Ji Hye

AU - Chung, Chung Soo

AU - Guo, Ding Ding

AU - Kim, Tae Gyu

AU - Choi, Yun Jaie

AU - Cho, Chong Su

N1 - Funding Information: This work was supported by the Agricultural R&D Promotion Center (ARPC) (2006-0053). We thank the National Instrumentation Center for Environmental Management (NICEM) and Research Institute of Pharmaceutical Sciences (RIPS) for measurement of PKC activation, ROS production, and NF-κB activation. We also thank Prof. Byung-Hoon Lee for technical assistance. H.S. Moon and J.H. Seo were supported by Brain Korea 21 (BK21) grant.

PY - 2007/5/18

Y1 - 2007/5/18

N2 - It has long been recognized that leptin, a hormone made by adipocytes, is an important circulating signal for the regulation of body weight. In addition, matrix metalloproteinase (MMP), especially MMP-2, an adipocyte-secreted protein which promotes multi-cellular adipose clusters, is up-regulated in obesity. The present study is designed to evaluate whether trans-10,cis-12 conjugated linoleic acid (t-CLA) can suppress leptin-induced MMP-2 secretion in 3T3-L1 cells. The result showed that expressions of adipocyte marker proteins were significantly reduced by t-CLA-treated cultures, but not by linoleic acid (LA)-treated ones. Interestingly, MMP-2 secretion was significantly increased by leptin-treated cultures, thereby leading to accelerate adipocyte differentiation, indicating that MMP-2 was a necessary mediator of adipogenesis. However, increasing concentration of t-CLA significantly reduced leptin-induced MMP-2 secretion and triglyceride (TG) content. These findings provide support for a role for t-CLA in the regulation of metabolism in leptin-induced adipose tissue development.

AB - It has long been recognized that leptin, a hormone made by adipocytes, is an important circulating signal for the regulation of body weight. In addition, matrix metalloproteinase (MMP), especially MMP-2, an adipocyte-secreted protein which promotes multi-cellular adipose clusters, is up-regulated in obesity. The present study is designed to evaluate whether trans-10,cis-12 conjugated linoleic acid (t-CLA) can suppress leptin-induced MMP-2 secretion in 3T3-L1 cells. The result showed that expressions of adipocyte marker proteins were significantly reduced by t-CLA-treated cultures, but not by linoleic acid (LA)-treated ones. Interestingly, MMP-2 secretion was significantly increased by leptin-treated cultures, thereby leading to accelerate adipocyte differentiation, indicating that MMP-2 was a necessary mediator of adipogenesis. However, increasing concentration of t-CLA significantly reduced leptin-induced MMP-2 secretion and triglyceride (TG) content. These findings provide support for a role for t-CLA in the regulation of metabolism in leptin-induced adipose tissue development.

KW - Conjugated linoleic acid

KW - Leptin

KW - Lipid accumulation

KW - Matrix metalloproteinase-2

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Leptin-induced matrix metalloproteinase-2 secretion is suppressed by trans-10,cis-12 conjugated linoleic acid

Abstract

Bibliographical note

Keywords

ASJC Scopus subject areas

UN SDGs

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