TY - JOUR
T1 - Lethal disease in infant and juvenile Syrian hamsters experimentally infected with Imjin virus, a newfound crocidurine shrew-borne hantavirus
AU - Gu, Se Hun
AU - Kim, Young Sik
AU - Baek, Luck Ju
AU - Kurata, Takeshi
AU - Yanagihara, Richard
AU - Song, Jin Won
N1 - Funding Information:
This work was supported in part by grants from the National Institute of Allergy and Infectious Diseases ( R01AI075057 ) and the National Institute of General Medical Sciences ( P20GM103516 ) of the National Institutes of Health , and the Global Emerging Infections Surveillance and Response System (GEIS), U.S. Department of Defense .
Publisher Copyright:
© 2015 Elsevier B.V.
PY - 2015/12/1
Y1 - 2015/12/1
N2 - To gain insights into the pathogenicity of Imjin virus (MJNV), a newfound hantavirus isolated from the Ussuri white-toothed shrew (Crocidura lasiura), groups of Syrian hamsters (Mesocricetus auratus) of varying ages (<. 1, 5, 10, 14, 21, 35 and 56. days) were inoculated by the intraperitoneal route with 1000 pfu of MJNV strains 04-55 and 05-11. MJNV-infected Syrian hamsters, aged 21. days or less, exhibited reduced activity, weight loss, respiratory distress, hind-limb paralysis and seizures. Death ensued 1 to 6. days after onset of clinical disease. MJNV RNA was detected in brain and other major organs by RT-PCR and real time-PCR. Histopathological examination showed alveolar hemorrhage, interstitial pneumonia and severe pulmonary congestion; focal hepatic necrosis and portal inflammation; and acute meningoencephalitis. By immunohistochemistry, MJNV antigen was detected in pulmonary microvascular endothelial cells and glial cells. Older hamsters (35 and 56. days of age) developed subclinical infection without histopathological changes. Future studies are warranted to determine the pathophysiologic bases for the differential age susceptibility of Syrian hamsters to lethal MJNV disease.
AB - To gain insights into the pathogenicity of Imjin virus (MJNV), a newfound hantavirus isolated from the Ussuri white-toothed shrew (Crocidura lasiura), groups of Syrian hamsters (Mesocricetus auratus) of varying ages (<. 1, 5, 10, 14, 21, 35 and 56. days) were inoculated by the intraperitoneal route with 1000 pfu of MJNV strains 04-55 and 05-11. MJNV-infected Syrian hamsters, aged 21. days or less, exhibited reduced activity, weight loss, respiratory distress, hind-limb paralysis and seizures. Death ensued 1 to 6. days after onset of clinical disease. MJNV RNA was detected in brain and other major organs by RT-PCR and real time-PCR. Histopathological examination showed alveolar hemorrhage, interstitial pneumonia and severe pulmonary congestion; focal hepatic necrosis and portal inflammation; and acute meningoencephalitis. By immunohistochemistry, MJNV antigen was detected in pulmonary microvascular endothelial cells and glial cells. Older hamsters (35 and 56. days of age) developed subclinical infection without histopathological changes. Future studies are warranted to determine the pathophysiologic bases for the differential age susceptibility of Syrian hamsters to lethal MJNV disease.
KW - Hantavirus
KW - Imjin virus
KW - Meningoencephalitis
KW - Syrian hamster
UR - http://www.scopus.com/inward/record.url?scp=84943605816&partnerID=8YFLogxK
U2 - 10.1016/j.meegid.2015.09.009
DO - 10.1016/j.meegid.2015.09.009
M3 - Article
C2 - 26371066
AN - SCOPUS:84943605816
SN - 1567-1348
VL - 36
SP - 231
EP - 239
JO - Infection, Genetics and Evolution
JF - Infection, Genetics and Evolution
ER -