Lighting up plants with near-infrared fluorescence probes

Yida Pang, Mengjiao Lu, Hyeonji Rha, Wenchao Yang, Amit Sharma, Yao Sun, Jong Seung Kim

Research output: Contribution to journalReview articlepeer-review


Fluorescence imaging is a non-invasive and highly sensitive bioimaging technique that has shown remarkable strides in plant science. It enables real-time monitoring and analysis of biological and pathological processes in plants by labeling specific molecular or cellular structures with fluorescent probes. However, tissue scattering and phytochrome interference have been obstacles for conventional fluorescence imaging of plants in the ultraviolet and visible spectrum, resulting in unsatisfactory imaging quality. Fortunately, advances in near-infrared (NIR) fluorescence imaging technology (650–900 nm) offer superior spatial-temporal resolution and reduced tissue scattering, which is sure to improve plant imaging quality. In this review, we summarize recent progress in the development of NIR fluorescence imaging probes and their applications for in vivo plant imaging and the identification of plant-related biomolecules. We hope this review provides a new perspective for plant science research and highlights NIR fluorescence imaging as a powerful tool for analyzing plant physiology, adaptive mechanisms, and coping with environmental stress in the near future.

Original languageEnglish
Pages (from-to)774-787
Number of pages14
JournalScience China Chemistry
Issue number3
Publication statusPublished - 2024 Mar

Bibliographical note

Publisher Copyright:
© Science China Press 2023.


  • NIR fluorescent probe
  • biologic application
  • imaging
  • plant

ASJC Scopus subject areas

  • General Chemistry


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