TY - JOUR
T1 - Limnobaculum parvum gen. Nov., sp. nov., isolated from a freshwater lake
AU - Baek, Chaeyun
AU - Shin, Su Kyoung
AU - Yi, Hana
N1 - Funding Information:
This study was supported by the research grant ‘Survey of Freshwater Organisms and Specimen Collection (Prokaryotes)’ from the Nakdonggang National Institute of Biological Resources of the Ministry of Environment in Korea, by the National Research Council of Science and Technology (NST) grant from the Korean government (MSIP) (No. CRC-16–01-KRICT) and by the Strategic Initiative for Microbiomes in Agriculture and Food, funded by the Ministry of Agriculture, Food and Rural Affairs (918010–4).
Funding Information:
This study was supported by the research grant ‘Survey of Freshwater Organisms and Specimen Collection (Prokaryotes)’ from the Nakdong-gang National Institute of Biological Resources of the Ministry of Environment in Korea, by the National Research Council of Science and Technology (NST) grant from the Korean government (MSIP) (No. CRC-16–01-KRICT) and by the Strategic Initiative for Microbiomes in Agriculture and Food, funded by the Ministry of Agriculture, Food and Rural Affairs (918010–4).
Publisher Copyright:
© 2019 IUMS.
PY - 2019/6
Y1 - 2019/6
N2 - A Gram-stain-negative, rod-shaped, facultatively anaerobic bacterium, designated as strain HYN0051T, was isolated from lake water. 16S rRNA gene sequence analyses indicated that the isolate shares the highest sequence similarity with the genus Pragia (97.1%) of the family Enterobacteriaceae. Strain HYN0051T did not form a rigid clade with the genus Pragia in any of the phylogenetic trees, demonstrating the novel generic status of the isolate. The genome of strain HYN0051T (CP029185) is a single circular chromosome of 3.84 Mb. The major fatty acids detected were C14: 0, C16: 0, summed feature 2 (C12: 0 aldehyde and/or unknown 10.928), summed feature 3 (C16: 1ω6c and/or C16: 1ω7c) and summed feature 8 (C18: 1ω 6c and/or C18: 1 ω 7c). The isoprenoid quinones were Q-8, MK-8 and DMK-8. The major polar lipids were phosphatidylglycerol, phosphatidylethanolamine and one unidentified phospholipid. The genome size, G+C content and fatty acid profile were very similar to that of the genus Pragia, but the composition of isoprenoid quinones and polar lipids, and numerous phenotypic properties also supported the distinctiveness of the new isolate from the genus Pragia. Thus, based on phylogenetic and phenotypic data, a novel species of a new genus, Limnobaculum parvum gen. nov., sp. nov., is proposed. The type strain of Limnobaculum parvum is HYN0051T (=KACC 19186T=NBRC 112742T).
AB - A Gram-stain-negative, rod-shaped, facultatively anaerobic bacterium, designated as strain HYN0051T, was isolated from lake water. 16S rRNA gene sequence analyses indicated that the isolate shares the highest sequence similarity with the genus Pragia (97.1%) of the family Enterobacteriaceae. Strain HYN0051T did not form a rigid clade with the genus Pragia in any of the phylogenetic trees, demonstrating the novel generic status of the isolate. The genome of strain HYN0051T (CP029185) is a single circular chromosome of 3.84 Mb. The major fatty acids detected were C14: 0, C16: 0, summed feature 2 (C12: 0 aldehyde and/or unknown 10.928), summed feature 3 (C16: 1ω6c and/or C16: 1ω7c) and summed feature 8 (C18: 1ω 6c and/or C18: 1 ω 7c). The isoprenoid quinones were Q-8, MK-8 and DMK-8. The major polar lipids were phosphatidylglycerol, phosphatidylethanolamine and one unidentified phospholipid. The genome size, G+C content and fatty acid profile were very similar to that of the genus Pragia, but the composition of isoprenoid quinones and polar lipids, and numerous phenotypic properties also supported the distinctiveness of the new isolate from the genus Pragia. Thus, based on phylogenetic and phenotypic data, a novel species of a new genus, Limnobaculum parvum gen. nov., sp. nov., is proposed. The type strain of Limnobaculum parvum is HYN0051T (=KACC 19186T=NBRC 112742T).
KW - Lake water
KW - Limnobaculum
KW - Nakdong River
UR - http://www.scopus.com/inward/record.url?scp=85067089809&partnerID=8YFLogxK
U2 - 10.1099/ijsem.0.003401
DO - 10.1099/ijsem.0.003401
M3 - Article
C2 - 30994436
AN - SCOPUS:85067089809
SN - 1466-5026
VL - 69
SP - 1826
EP - 1830
JO - International Journal of Systematic and Evolutionary Microbiology
JF - International Journal of Systematic and Evolutionary Microbiology
IS - 6
ER -