Linoleic acid inhibits Pseudomonas aeruginosa biofilm formation by activating diffusible signal factor-mediated quorum sensing

Han Shin Kim, Eunji Cha, So Young Ham, Jeong Hoon Park, Sang Jin Nam, Hongmok Kwon, Youngjoo Byun, Hee Deung Park

Research output: Contribution to journalArticlepeer-review

19 Citations (Scopus)

Abstract

Bacterial biofilm formation causes serious problems in various fields of medical, clinical, and industrial settings. Antibiotics and biocide treatments are typical methods used to remove bacterial biofilms, but biofilms are difficult to remove effectively from surfaces due to their increased resistance. An alternative approach to treatment with antimicrobial agents is using biofilm inhibitors that regulate biofilm development without inhibiting bacterial growth. In the present study, we found that linoleic acid (LA), a plant unsaturated fatty acid, inhibits biofilm formation under static and continuous conditions without inhibiting the growth of Pseudomonas aeruginosa. LA also influenced the bacterial motility, extracellular polymeric substance production, and biofilm dispersion by decreasing the intracellular cyclic diguanylate concentration through increased phosphodiesterase activity. Furthermore, quantitative gene expression analysis demonstrated that LA induced the expression of genes associated with diffusible signaling factor-mediated quorum sensing that can inhibit or induce the dispersion of P. aeruginosa biofilms. These results suggest that LA is functionally and structurally similar to a P. aeruginosa diffusible signaling factor (cis-2-decenoic acid) and, in turn, act as an agonist molecule in biofilm dispersion.

Original languageEnglish
Pages (from-to)82-93
Number of pages12
JournalBiotechnology and Bioengineering
Volume118
Issue number1
DOIs
Publication statusPublished - 2021 Jan

Bibliographical note

Funding Information:
This work was supported by the Korea Environment Industry & Technology Institute (KEITI) through Subsurface Environmental Management (SEM) Projects funded by Korea Ministry of Environment (MOE) (1485016734). The authors would like to thank Ted Inpyo Hong for generating the preliminary linoleic acid biofilm inhibition result, Prof. Joon-Hee Lee from Pusan National University for kindly providing the CdrA reporter strains, and Prof. You-Hee Cho from Cha University for kindly providing the PA14 mutants strains (ΔrpfF and ΔrpfC).

Funding Information:
This work was supported by the Korea Environment Industry & Technology Institute (KEITI) through Subsurface Environmental Management (SEM) Projects funded by Korea Ministry of Environment (MOE) (1485016734). The authors would like to thank Ted Inpyo Hong for generating the preliminary linoleic acid biofilm inhibition result, Prof. Joon‐Hee Lee from Pusan National University for kindly providing the CdrA reporter strains, and Prof. You‐Hee Cho from Cha University for kindly providing the PA14 mutants strains ( and ). ΔrpfF ΔrpfC

Publisher Copyright:
© 2020 Wiley Periodicals LLC

Keywords

  • Pseudomonas aeruginosa
  • biofilm formation
  • diffusible signal factor
  • linoleic acid
  • quorum sensing

ASJC Scopus subject areas

  • Biotechnology
  • Bioengineering
  • Applied Microbiology and Biotechnology

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