TY - JOUR
T1 - Lipase-catalyzed acidolysis of menhaden oil with pinolenic acid
AU - Kim, In Hwan
AU - Hill, Charles G.
N1 - Funding Information:
This work was supported by the University of Wisconsin Sea Grant Institute under grants from the National Sea Grant College Program, National Oceanic and Atmospheric Administration, U.S. Department of Commerce, and from the State of Wisconsin (Federal grant number NA 46 RG 0481, project number R/AQ-34). Additional support was provided by National Science Foundation Grant BES-077524. We also thank Iswandi Jarto for his contributions to the experimental work.
Copyright:
Copyright 2011 Elsevier B.V., All rights reserved.
PY - 2006/2
Y1 - 2006/2
N2 - Lipase-catalyzed acidolysis of menhaden oil with a pinolenic acid (PLA) concentrate, prepared from pine nut oil, was studied in a solvent-free system. The PLA concentrate was prepared by urea complexation of the FA obtained by saponification of pine nut oil. Eight commercial lipases from different sources were screened for their ability to catalyze the acidolysis reaction. Two different types of structured lipids (SL) were synthesized. The first type, which has PLA residues as a primary FA residue at the sn-1,3 positions of the TAG, was synthesized using a 1,3-regiospecific lipase, namely, Lipozyme RM IM from Rhizomucor miehei. The second type of SL, which has PLA residues as a primary FA residue at both the sn-1,3 and sn-2 positions of the TAG, was synthesized using a nonspecific lipase, namely, Novozym 435 from Candida antarctica. The effects of variations in enzyme loading, temperature, and reaction time on PLA incorporation into the oil were monitored by GC analyses. The optimal temperature and enzyme loading for synthesis of the two types of SL were 50°C and 10% of the total weight of substrates for both enzymes. The optimal reaction time for the synthesis with Lipozyme RM IM was 16 h, whereas the optimal reaction time for the synthesis mediated by Novozym 435 was 36 h. Pancreatic lipase-catalyzed sn-2 positional analyses were also carried out on the TAG samples.
AB - Lipase-catalyzed acidolysis of menhaden oil with a pinolenic acid (PLA) concentrate, prepared from pine nut oil, was studied in a solvent-free system. The PLA concentrate was prepared by urea complexation of the FA obtained by saponification of pine nut oil. Eight commercial lipases from different sources were screened for their ability to catalyze the acidolysis reaction. Two different types of structured lipids (SL) were synthesized. The first type, which has PLA residues as a primary FA residue at the sn-1,3 positions of the TAG, was synthesized using a 1,3-regiospecific lipase, namely, Lipozyme RM IM from Rhizomucor miehei. The second type of SL, which has PLA residues as a primary FA residue at both the sn-1,3 and sn-2 positions of the TAG, was synthesized using a nonspecific lipase, namely, Novozym 435 from Candida antarctica. The effects of variations in enzyme loading, temperature, and reaction time on PLA incorporation into the oil were monitored by GC analyses. The optimal temperature and enzyme loading for synthesis of the two types of SL were 50°C and 10% of the total weight of substrates for both enzymes. The optimal reaction time for the synthesis with Lipozyme RM IM was 16 h, whereas the optimal reaction time for the synthesis mediated by Novozym 435 was 36 h. Pancreatic lipase-catalyzed sn-2 positional analyses were also carried out on the TAG samples.
KW - Acidolysis
KW - Lipase
KW - Menhaden oil
KW - Pine nut oil
KW - Pinolenic acid (PLA)
KW - Urea complexation
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U2 - 10.1007/s11746-006-1182-2
DO - 10.1007/s11746-006-1182-2
M3 - Article
AN - SCOPUS:33645279251
SN - 0003-021X
VL - 83
SP - 109
EP - 115
JO - JAOCS, Journal of the American Oil Chemists' Society
JF - JAOCS, Journal of the American Oil Chemists' Society
IS - 2
ER -