Lipase-catalyzed acidolysis of perilla oil with caprylic acid to produce structured lipids

In Hwan Kim, Hakryul Kim, Ki Teak Lee, Soo Hyun Chung, Soon Nam Ko

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65 Citations (Scopus)


Structured lipids were synthesized by acidolysis of perilla oil and caprylic acid using two lipases, Lipozyme RM IM from Rhizomucor miehei and Lipozyme TL IM from Thermomyces lanuginosa. Effects of molar ratio, reaction time, reaction temperature, enzyme load, and solvent content on acidolysis reactions were studied. The solvent content ranged from 0.0 (solvent-free) to 85.3%. The results showed that the incorporation increased in parallel with solvent content to 49.0% with Lipozyme RM IM and to 63.8% with Lipozyme TL IM. After 24 h incubation in n-hexane, caprylic acids were incorporated to 48.5 mol% with Lipozyme RM IM and to 51.4 mol% with Lipozyme TL IM, respectively, whereas linolenic acid content was reduced from 61.4 to 31.5 mol% with Lipozyme RM IM and to 28.4 mol% with Lipozyme TL IM, respectively. Lipozyme TL IM showed a higher acyl migration rate than Lipozyme RM IM when acidolysis was performed in the reaction system containing n-hexane as a solvent, whereas the difference in acyl migration between the two lipases in the solvent-free system was negligible.

Original languageEnglish
Pages (from-to)363-367
Number of pages5
JournalJAOCS, Journal of the American Oil Chemists' Society
Issue number4
Publication statusPublished - 2002 Apr

Bibliographical note

Funding Information:
This work was supported by Korea Research Foundation Grant KRF-2000-003-G00070.


  • Acidolysis
  • Acyl migration
  • Caprylic acid
  • Lipase
  • Perilla oil
  • Structured lipids

ASJC Scopus subject areas

  • General Chemical Engineering
  • Organic Chemistry


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