Lipase-catalyzed synthesis of 2-ethylhexyl palmitate in a solvent free system using step changes in temperature

Suhyeon Choi, Byung Hee Kim, Da Som No, Sung Won Yoon, Moon Won Lee, Dong Joong Im, In Hwan Kim

    Research output: Contribution to journalArticlepeer-review

    8 Citations (Scopus)

    Abstract

    Lipase-catalyzed synthesis of 2-ethylhexyl palmitate (2-EHP) from 2-ethylhexyl alcohol and palmitic acid was carried out in a solvent-free system. A commercial liquid lipase (Eversa Transform 2.0, Novozymes) from Thermomyces lanuginosus was immobilized on Lewatit VP OC 1600, a macroporous hydrophobic carrier. The efficacy of this Eversa immobilized lipase prepared in this study was evaluated on the synthesis of 2-EHP compared with that of Novozym 435 (from Candida antarctica), Lipozyme RM IM (from Rhizomucor miehei), Lipozyme TL IM (from Thermomyces lanuginosus), and the liquid Eversa lipase (commercial name: Eversa Transform 2.0). Among these lipases, the Eversa immobilized lipase was the most effective for the synthesis of 2-EHP. Optimum conditions for the synthesis of 2-EHP using this enzyme were a temperature of 55 °C and enzyme loading of 2% (based on the total weight of substrate). The conversion of 97% was achieved under these optimum conditions. Finally, as a cost-saving strategy, a step change in the reaction temperature was introduced. When a step change in the reaction temperature between 55 °C and 45 °C used, an identical degree of conversion was achieved compared to a constant reaction temperature of 55 °C throughout the reaction.

    Original languageEnglish
    Article number108261
    JournalBiochemical Engineering Journal
    Volume177
    DOIs
    Publication statusPublished - 2022 Jan

    Bibliographical note

    Funding Information:
    This research was supported by a grant of the Korea Health Technology R&D Project through the Korea Health Industry Development Institute (KHIDI), funded by the Ministry of Health and Welfare, Republic of Korea (Grant no.: HP20C0064 ).

    Publisher Copyright:
    © 2021 Elsevier B.V.

    Keywords

    • 2-Ethylhexyl palmitate
    • Eversa immobilized lipase
    • Hydrophobic carrier
    • Step change
    • Temperature

    ASJC Scopus subject areas

    • Biotechnology
    • Bioengineering
    • Environmental Engineering
    • Biomedical Engineering

    Fingerprint

    Dive into the research topics of 'Lipase-catalyzed synthesis of 2-ethylhexyl palmitate in a solvent free system using step changes in temperature'. Together they form a unique fingerprint.

    Cite this