Abstract
Lipase-catalyzed synthesis of 2-ethylhexyl palmitate (2-EHP) from 2-ethylhexyl alcohol and palmitic acid was carried out in a solvent-free system. A commercial liquid lipase (Eversa Transform 2.0, Novozymes) from Thermomyces lanuginosus was immobilized on Lewatit VP OC 1600, a macroporous hydrophobic carrier. The efficacy of this Eversa immobilized lipase prepared in this study was evaluated on the synthesis of 2-EHP compared with that of Novozym 435 (from Candida antarctica), Lipozyme RM IM (from Rhizomucor miehei), Lipozyme TL IM (from Thermomyces lanuginosus), and the liquid Eversa lipase (commercial name: Eversa Transform 2.0). Among these lipases, the Eversa immobilized lipase was the most effective for the synthesis of 2-EHP. Optimum conditions for the synthesis of 2-EHP using this enzyme were a temperature of 55 °C and enzyme loading of 2% (based on the total weight of substrate). The conversion of 97% was achieved under these optimum conditions. Finally, as a cost-saving strategy, a step change in the reaction temperature was introduced. When a step change in the reaction temperature between 55 °C and 45 °C used, an identical degree of conversion was achieved compared to a constant reaction temperature of 55 °C throughout the reaction.
Original language | English |
---|---|
Article number | 108261 |
Journal | Biochemical Engineering Journal |
Volume | 177 |
DOIs | |
Publication status | Published - 2022 Jan |
Bibliographical note
Funding Information:This research was supported by a grant of the Korea Health Technology R&D Project through the Korea Health Industry Development Institute (KHIDI), funded by the Ministry of Health and Welfare, Republic of Korea (Grant no.: HP20C0064 ).
Publisher Copyright:
© 2021 Elsevier B.V.
Keywords
- 2-Ethylhexyl palmitate
- Eversa immobilized lipase
- Hydrophobic carrier
- Step change
- Temperature
ASJC Scopus subject areas
- Biotechnology
- Bioengineering
- Environmental Engineering
- Biomedical Engineering