Lipase from Penicillium camembertii KCCM 11268: Optimization of solid state fermentation and application to biodiesel production

Waraporn Malilas, Seong Woo Kang, Sung Bong Kim, Hah Young Yoo, Warawut Chulalaksananukul, Seung Wook Kim

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25 Citations (Scopus)


Lipase was produced by Penicillium camembertii KCCM 11268 under solid state fermentation (SSF), and the production process was optimized by using statistical experimental designs. The initial moisture content, cultivation time, inoculum size and concentration of basal medium were considered as the factors of optimum conditions for SSF. P. Camembertii KCCM 11268 was cultivated in SSF using wheat bran as the substrate for lipase production. Under the optimized condition, lipase activity was reached around 7. 8 U/ml after eight days fermentation. To partially purify the lipase, ammonium sulfate (80% saturation) was added to the crude lipase solution and concentrated using a diafiltration (VIVAFLOW 50). The concentrated lipase solution from P. Camembertii KCCM 11268 (PCL) was immobilized on silica gel by cross-linking method. Also, PCL was mixed with a commercial lipase solution from Candida rugosa (CRL), and this mixture was co-immobilized on silica gel. The immobilized and co-immobilized lipase activities were 1150. 1 and 7924. 8 U/g matrix, respectively. Palm oil and methanol were used as substrates and 1mmol of methanol was added every 1. 5 h and 2 times during biodiesel production. The reaction was carried out at temperatures of 30, 40, 50, 60 and 70 °C. The maximum biodiesel conversion by co-immobilized lipase was 99% after 5 h at 50 °C.

Original languageEnglish
Pages (from-to)405-412
Number of pages8
JournalKorean Journal of Chemical Engineering
Issue number2
Publication statusPublished - 2013 Feb

Bibliographical note

Funding Information:
This work was supported by the Advanced Biomass R&D Center (ABC) of Global Frontier Project funded by the Ministry of Education, Science and Technology (ABC-2011-0031360), and was supported by grants from the Korea Foundation for Advanced Studies (KFAS).


  • Biodiesel Production
  • Immobilization
  • Lipase Production
  • Penicillium camembertii KCCM 11268
  • Solid State Fermentation
  • Statistical Experimental Design

ASJC Scopus subject areas

  • General Chemistry
  • General Chemical Engineering


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