Lipase immobilization on silica gel using a cross-linking method

Dong Hwan Lee, Cheol Hee Park, Jong Mo Yeo, Seung Wook Kim

Research output: Contribution to journalArticlepeer-review

70 Citations (Scopus)


The objective of this study was to develop a suitable method for immobilizing lipase. Although many investigators have studied lipase immobilization, detailed optimization of each step for lipase immobilization using cross-linking methods has been lacking. Therefore, we focused on establishing and optimizing each step of the immobilization procedure to improve procedural efficiencies. Lipase produced from Rhizopus oryzae was immobilized on silica gel using a cross-linking method. The immobilization procedures, such as the pretreatment of silica gel, silanization, crosslinking, glutaraldehyde modification, and elimination of unreated aldehyde groups, were all optimized. Furthermore, the stability of the immobilized lipase and its suitability for reuse were also evaluated.

Original languageEnglish
Pages (from-to)777-782
Number of pages6
JournalJournal of Industrial and Engineering Chemistry
Issue number5
Publication statusPublished - 2006 Sept


  • Glutaraldehyde
  • Immobilization
  • Lipase
  • Reuse
  • Silanization

ASJC Scopus subject areas

  • General Chemical Engineering


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