Lipase-mediated synthesis of neopentyl glycol diester using a combination of reduced and standard pressure

Ji Won Kim, Byung Hee Kim, Yangha Kim, Moon Won Lee, Dong Joong Im, In Hwan Kim

Research output: Contribution to journalArticlepeer-review

10 Citations (Scopus)


Neopentyl glycol (NPG) diester is a biodegradable lubricant used in the machinery industry as it is suitable for the low-temperature environment. NPG diester was successfully synthesized with fatty acid and NPG using immobilized lipase. Immobilization was carried out with a liquid enzyme, Eversa® Transform 2.0 (from Thermomyces lanuginosus) and Lewatit VP OC 1600 as a carrier. The Eversa immobilized lipase prepared in this study was compared with commercial lipases, such as Novozym 435, Lipozyme RM IM, and Lipozyme TL IM. The Eversa immobilized lipase was the most effective biocatalyst for the synthesis of NPG diesters. The effects of enzyme loading and temperature on conversion were explored at a standard pressure of 101.3 kPa. And then, the effect of reduced pressure was investigated in the range of 1.3 to 40.0 kPa under optimum conditions obtained at standard pressure. The maximum conversion of ca. 63% was achieved at the optimal temperature of 50°C and the enzyme loading of 5% (based on the total weight of the substrate) at standard pressure after 10 h. However, the conversion to NPG diester increased markedly up to 97% after 10 h, even though reduced pressure as low as 26.7 kPa was applied. No significant differences in the conversion to NPG diester between the reactions at constant reduced pressure and the combined reaction of reduced pressure and standard pressure were observed. This pressure combination method employed in this study demonstrated a novel, energy-saving strategy for the synthesis of NPG diester.

Original languageEnglish
Pages (from-to)1001-1007
Number of pages7
JournalJAOCS, Journal of the American Oil Chemists' Society
Issue number10
Publication statusPublished - 2021 Oct

Bibliographical note

Funding Information:
This research was supported by a grant of the Korea Health Technology R&D Project through the Korea Health Industry Development Institute (KHIDI), funded by the Ministry of Health and Welfare, Republic of Korea (grant number: HP20C0064).

Publisher Copyright:
© 2021 AOCS.


  • biolubricant
  • esterification
  • immobilized lipase
  • neopentyl glycol diester
  • reduced pressure

ASJC Scopus subject areas

  • General Chemical Engineering
  • Organic Chemistry


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