Lipid raft proteome reveals that oxidative phosphorylation system is associated with the plasma membrane

Bong Woo Kim, Chang Seok Lee, Jae Sung Yi, Joo Hyung Lee, Joong Won Lee, Hyo Jung Choo, Soon Young Jung, Min Sik Kim, Sang Won Lee, Myung Shik Lee, Gyesoon Yoon, Young Gyu Ko

Research output: Contribution to journalReview articlepeer-review

27 Citations (Scopus)


Although accumulating proteomic analyses have supported the fact that mitochondrial oxidative phosphorylation (OXPHOS) complexes are localized in lipid rafts, which mediate cell signaling, immune response and host-pathogen interactions, there has been no in-depth study of the physiological functions of lipid-raft OXPHOS complexes. Here, we show that many subunits of OXPHOS complexes were identified from the lipid rafts of human adipocytes, C2C12 myotubes, Jurkat cells and surface biotin-labeled Jurkat cells via shotgun proteomic analysis. We discuss the findings of OXPHOS complexes in lipid rafts, the role of the surface ATP synthase complex as a receptor for various ligands and extracellular superoxide generation by plasma membrane oxidative phosphorylation complexes.

Original languageEnglish
Pages (from-to)849-866
Number of pages18
JournalExpert Review of Proteomics
Issue number6
Publication statusPublished - 2010 Dec

Bibliographical note

Funding Information:
This work was supported by grants awarded to Young-Gyu Ko from the Basic Science Program (C00286) and Basic Research Laboratory Program (0001197) of the Korea Research Foundation, and National R&D Program for Cancer Control, Korea Ministry of Health, Welfare and Family Affairs (09201800). The authors have no other relevant affiliations or financial involvement with any organization or entity with a financial interest in or financial conflict with the subject matter or materials discussed in the manuscript apart from those disclosed.


  • ATP synthase
  • detergent-resistant lipid rafts
  • extracellular ATP
  • extracellular superoxide
  • oxidative phosphorylation complexes
  • oxygen consumption

ASJC Scopus subject areas

  • Biochemistry
  • Molecular Biology


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