Lipid raft proteome reveals that oxidative phosphorylation system is associated with the plasma membrane

Bong Woo Kim; Chang Seok Lee; Jae Sung Yi; Joo Hyung Lee; Joong Won Lee; Hyo Jung Choo; Soon Young Jung; Min Sik Kim; Sang Won Lee; Myung Shik Lee; Gyesoon Yoon; Young Gyu Ko

doi:10.1586/epr.10.87

Lipid raft proteome reveals that oxidative phosphorylation system is associated with the plasma membrane

Bong Woo Kim, Chang Seok Lee, Jae Sung Yi, Joo Hyung Lee, Joong Won Lee, Hyo Jung Choo, Soon Young Jung, Min Sik Kim, Sang Won Lee, Myung Shik Lee, Gyesoon Yoon, Young Gyu Ko

Research output: Contribution to journal › Review article › peer-review

27 Citations (Scopus)

Abstract

Although accumulating proteomic analyses have supported the fact that mitochondrial oxidative phosphorylation (OXPHOS) complexes are localized in lipid rafts, which mediate cell signaling, immune response and host-pathogen interactions, there has been no in-depth study of the physiological functions of lipid-raft OXPHOS complexes. Here, we show that many subunits of OXPHOS complexes were identified from the lipid rafts of human adipocytes, C2C12 myotubes, Jurkat cells and surface biotin-labeled Jurkat cells via shotgun proteomic analysis. We discuss the findings of OXPHOS complexes in lipid rafts, the role of the surface ATP synthase complex as a receptor for various ligands and extracellular superoxide generation by plasma membrane oxidative phosphorylation complexes.

Original language	English
Pages (from-to)	849-866
Number of pages	18
Journal	Expert Review of Proteomics
Volume	7
Issue number	6
DOIs	https://doi.org/10.1586/epr.10.87
Publication status	Published - 2010 Dec

Keywords

ATP synthase
detergent-resistant lipid rafts
extracellular ATP
extracellular superoxide
oxidative phosphorylation complexes
oxygen consumption

ASJC Scopus subject areas

Biochemistry
Molecular Biology

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10.1586/epr.10.87

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@article{e37409533ce84518a498ccf8f4aff1a8,

title = "Lipid raft proteome reveals that oxidative phosphorylation system is associated with the plasma membrane",

abstract = "Although accumulating proteomic analyses have supported the fact that mitochondrial oxidative phosphorylation (OXPHOS) complexes are localized in lipid rafts, which mediate cell signaling, immune response and host-pathogen interactions, there has been no in-depth study of the physiological functions of lipid-raft OXPHOS complexes. Here, we show that many subunits of OXPHOS complexes were identified from the lipid rafts of human adipocytes, C2C12 myotubes, Jurkat cells and surface biotin-labeled Jurkat cells via shotgun proteomic analysis. We discuss the findings of OXPHOS complexes in lipid rafts, the role of the surface ATP synthase complex as a receptor for various ligands and extracellular superoxide generation by plasma membrane oxidative phosphorylation complexes.",

keywords = "ATP synthase, detergent-resistant lipid rafts, extracellular ATP, extracellular superoxide, oxidative phosphorylation complexes, oxygen consumption",

author = "Kim, {Bong Woo} and Lee, {Chang Seok} and Yi, {Jae Sung} and Lee, {Joo Hyung} and Lee, {Joong Won} and Choo, {Hyo Jung} and Jung, {Soon Young} and Kim, {Min Sik} and Lee, {Sang Won} and Lee, {Myung Shik} and Gyesoon Yoon and Ko, {Young Gyu}",

note = "Funding Information: This work was supported by grants awarded to Young-Gyu Ko from the Basic Science Program (C00286) and Basic Research Laboratory Program (0001197) of the Korea Research Foundation, and National R&D Program for Cancer Control, Korea Ministry of Health, Welfare and Family Affairs (09201800). The authors have no other relevant affiliations or financial involvement with any organization or entity with a financial interest in or financial conflict with the subject matter or materials discussed in the manuscript apart from those disclosed.",

year = "2010",

month = dec,

doi = "10.1586/epr.10.87",

language = "English",

volume = "7",

pages = "849--866",

journal = "Expert Review of Proteomics",

issn = "1478-9450",

publisher = "Expert Reviews Ltd.",

number = "6",

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TY - JOUR

T1 - Lipid raft proteome reveals that oxidative phosphorylation system is associated with the plasma membrane

AU - Kim, Bong Woo

AU - Lee, Chang Seok

AU - Yi, Jae Sung

AU - Lee, Joo Hyung

AU - Lee, Joong Won

AU - Choo, Hyo Jung

AU - Jung, Soon Young

AU - Kim, Min Sik

AU - Lee, Sang Won

AU - Lee, Myung Shik

AU - Yoon, Gyesoon

AU - Ko, Young Gyu

N1 - Funding Information: This work was supported by grants awarded to Young-Gyu Ko from the Basic Science Program (C00286) and Basic Research Laboratory Program (0001197) of the Korea Research Foundation, and National R&D Program for Cancer Control, Korea Ministry of Health, Welfare and Family Affairs (09201800). The authors have no other relevant affiliations or financial involvement with any organization or entity with a financial interest in or financial conflict with the subject matter or materials discussed in the manuscript apart from those disclosed.

PY - 2010/12

Y1 - 2010/12

N2 - Although accumulating proteomic analyses have supported the fact that mitochondrial oxidative phosphorylation (OXPHOS) complexes are localized in lipid rafts, which mediate cell signaling, immune response and host-pathogen interactions, there has been no in-depth study of the physiological functions of lipid-raft OXPHOS complexes. Here, we show that many subunits of OXPHOS complexes were identified from the lipid rafts of human adipocytes, C2C12 myotubes, Jurkat cells and surface biotin-labeled Jurkat cells via shotgun proteomic analysis. We discuss the findings of OXPHOS complexes in lipid rafts, the role of the surface ATP synthase complex as a receptor for various ligands and extracellular superoxide generation by plasma membrane oxidative phosphorylation complexes.

AB - Although accumulating proteomic analyses have supported the fact that mitochondrial oxidative phosphorylation (OXPHOS) complexes are localized in lipid rafts, which mediate cell signaling, immune response and host-pathogen interactions, there has been no in-depth study of the physiological functions of lipid-raft OXPHOS complexes. Here, we show that many subunits of OXPHOS complexes were identified from the lipid rafts of human adipocytes, C2C12 myotubes, Jurkat cells and surface biotin-labeled Jurkat cells via shotgun proteomic analysis. We discuss the findings of OXPHOS complexes in lipid rafts, the role of the surface ATP synthase complex as a receptor for various ligands and extracellular superoxide generation by plasma membrane oxidative phosphorylation complexes.

KW - ATP synthase

KW - detergent-resistant lipid rafts

KW - extracellular ATP

KW - extracellular superoxide

KW - oxidative phosphorylation complexes

KW - oxygen consumption

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U2 - 10.1586/epr.10.87

DO - 10.1586/epr.10.87

M3 - Review article

C2 - 21142887

AN - SCOPUS:78650433384

SN - 1478-9450

VL - 7

SP - 849

EP - 866

JO - Expert Review of Proteomics

JF - Expert Review of Proteomics

IS - 6

ER -

Lipid raft proteome reveals that oxidative phosphorylation system is associated with the plasma membrane

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Keywords

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