We examined the effects of Lithospermum erythrorhizon Sieb. et Zucc. (LE) on cholesterol metabolism in vitro. The ethanolic LE extract (ELE) had total polyphenolic and flavonoid contents of 353±7 and 285±51mg/dL, respectively. The ELE inhibited Cu2+-mediated LDL oxidation at <400μg/mL. In HepG2 cells treated with 400μg/mL ELE, the expression of 3-hydroxy-3-methyl-glutaryl-CoA reductase decreased markedly (45%; P<0.05), whereas that of the LDL receptor increased (230%; P<0.05). The protein levels of both were altered similarly. The ELE also increased membrane-bound and cell-associated LDL particles, possibly via upregulation of the LDL receptor. In hepatocytes, 400μg/mL ELE affected surrogate markers of HDL and LDL synthesis: significant expression of apolipoprotein A-I was induced (20%; P<0.05), whereas that of apoB was suppressed (30%; P<0.05). In conclusion, ELE may improve cellular cholesterol metabolism by inhibiting cholesterol biosynthesis and apoB production, accelerating plasma LDL uptake and reducing LDL oxidation.
|Number of pages||17|
|Journal||Journal of Food Biochemistry|
|Publication status||Published - 2011 Jun|
ASJC Scopus subject areas
- Food Science
- Cell Biology