Live-cell imaging and profiling of c-Jun N-terminal kinases using covalent inhibitor-derived probes

Linghui Qian, Sijun Pan, Jun Seok Lee, Jingyan Ge, Lin Li, Shao Q. Yao

Research output: Contribution to journalArticlepeer-review

12 Citations (Scopus)

Abstract

c-Jun N-terminal kinases (JNKs) are involved in critical cellular functions. Herein, small-molecule JNK-targeting probes are reported based on a covalent inhibitor. Together with newly developed two-photon fluorescence Turn-ON reporters and chemoproteomic studies, we showed that some probes may be suitable for live-cell imaging and profiling of JNKs.

Original languageEnglish
Pages (from-to)1092-1095
Number of pages4
JournalChemical Communications
Volume55
Issue number8
DOIs
Publication statusPublished - 2019
Externally publishedYes

Bibliographical note

Funding Information:
We thank Dr Hailong Zhang (NUS) for his help with chemical synthesis. Financial support was provided by the Synthetic Biology Research & Development Programme (SBP) of National Research Foundation (NRF), the GSK-EDB Trust Fund (R-143-000-688-592), MOE-T1 (R-143-000-694-114) of Singapore, the National Natural Science Foundation of China (21877100, 21708034, 81672508 and 61505076) and the Jiangsu Provincial Foundation for Distinguished Young Scholars (BK20170041).

Publisher Copyright:
© The Royal Society of Chemistry.

ASJC Scopus subject areas

  • Catalysis
  • Electronic, Optical and Magnetic Materials
  • Ceramics and Composites
  • General Chemistry
  • Surfaces, Coatings and Films
  • Metals and Alloys
  • Materials Chemistry

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