c-Jun N-terminal kinases (JNKs) are involved in critical cellular functions. Herein, small-molecule JNK-targeting probes are reported based on a covalent inhibitor. Together with newly developed two-photon fluorescence Turn-ON reporters and chemoproteomic studies, we showed that some probes may be suitable for live-cell imaging and profiling of JNKs.
Bibliographical noteFunding Information:
We thank Dr Hailong Zhang (NUS) for his help with chemical synthesis. Financial support was provided by the Synthetic Biology Research & Development Programme (SBP) of National Research Foundation (NRF), the GSK-EDB Trust Fund (R-143-000-688-592), MOE-T1 (R-143-000-694-114) of Singapore, the National Natural Science Foundation of China (21877100, 21708034, 81672508 and 61505076) and the Jiangsu Provincial Foundation for Distinguished Young Scholars (BK20170041).
© The Royal Society of Chemistry.
ASJC Scopus subject areas
- Electronic, Optical and Magnetic Materials
- Ceramics and Composites
- Surfaces, Coatings and Films
- Metals and Alloys
- Materials Chemistry