Loading device effect on protein unfolding mechanics

Gwonchan Yoon; Sungsoo Na; Kilho Eom

doi:10.1063/1.4732798

Loading device effect on protein unfolding mechanics

Gwonchan Yoon, Sungsoo Na, Kilho Eom

Research output: Contribution to journal › Article › peer-review

9 Citations (Scopus)

Abstract

Single-molecule mechanical manipulation has enabled quantitative understanding of not only the kinetics of both bond rupture and protein unfolding, but also the free energy landscape of chemical bond andor protein folding. Despite recent studies reporting the role of loading device in bond rupture, a loading device effect on protein unfolding mechanics has not been well studied. In this work, we have studied the effect of loading-device stiffness on the kinetics of both bond rupture and protein unfolding mechanics using Brownian dynamics simulations. It is shown that bond rupture forces are dependent on not only loading rate but also the stiffness of loading device, and that protein unfolding mechanics is highly correlated with the stiffness of loading device. Our study sheds light on the importance of loading device effect on the mechanically induced bond ruptures and protein unfolding.

Original language	English
Article number	025102
Journal	Journal of Chemical Physics
Volume	137
Issue number	2
DOIs	https://doi.org/10.1063/1.4732798
Publication status	Published - 2012 Jul 14

ASJC Scopus subject areas

Physics and Astronomy(all)
Physical and Theoretical Chemistry

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10.1063/1.4732798

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abstract = "Single-molecule mechanical manipulation has enabled quantitative understanding of not only the kinetics of both bond rupture and protein unfolding, but also the free energy landscape of chemical bond andor protein folding. Despite recent studies reporting the role of loading device in bond rupture, a loading device effect on protein unfolding mechanics has not been well studied. In this work, we have studied the effect of loading-device stiffness on the kinetics of both bond rupture and protein unfolding mechanics using Brownian dynamics simulations. It is shown that bond rupture forces are dependent on not only loading rate but also the stiffness of loading device, and that protein unfolding mechanics is highly correlated with the stiffness of loading device. Our study sheds light on the importance of loading device effect on the mechanically induced bond ruptures and protein unfolding.",

author = "Gwonchan Yoon and Sungsoo Na and Kilho Eom",

note = "Funding Information: This work was supported by National Research Foundation of Korea under Grant No. NRF-2010-0026223 (to K.E.), and Grant No. 2011-0007272, 2011-0029477, 2012-0000785 (to S.N.), as well as Korea University Grant (to G.Y. and S.N). Copyright: Copyright 2012 Elsevier B.V., All rights reserved.",

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AU - Yoon, Gwonchan

AU - Na, Sungsoo

AU - Eom, Kilho

N1 - Funding Information: This work was supported by National Research Foundation of Korea under Grant No. NRF-2010-0026223 (to K.E.), and Grant No. 2011-0007272, 2011-0029477, 2012-0000785 (to S.N.), as well as Korea University Grant (to G.Y. and S.N). Copyright: Copyright 2012 Elsevier B.V., All rights reserved.

PY - 2012/7/14

Y1 - 2012/7/14

N2 - Single-molecule mechanical manipulation has enabled quantitative understanding of not only the kinetics of both bond rupture and protein unfolding, but also the free energy landscape of chemical bond andor protein folding. Despite recent studies reporting the role of loading device in bond rupture, a loading device effect on protein unfolding mechanics has not been well studied. In this work, we have studied the effect of loading-device stiffness on the kinetics of both bond rupture and protein unfolding mechanics using Brownian dynamics simulations. It is shown that bond rupture forces are dependent on not only loading rate but also the stiffness of loading device, and that protein unfolding mechanics is highly correlated with the stiffness of loading device. Our study sheds light on the importance of loading device effect on the mechanically induced bond ruptures and protein unfolding.

AB - Single-molecule mechanical manipulation has enabled quantitative understanding of not only the kinetics of both bond rupture and protein unfolding, but also the free energy landscape of chemical bond andor protein folding. Despite recent studies reporting the role of loading device in bond rupture, a loading device effect on protein unfolding mechanics has not been well studied. In this work, we have studied the effect of loading-device stiffness on the kinetics of both bond rupture and protein unfolding mechanics using Brownian dynamics simulations. It is shown that bond rupture forces are dependent on not only loading rate but also the stiffness of loading device, and that protein unfolding mechanics is highly correlated with the stiffness of loading device. Our study sheds light on the importance of loading device effect on the mechanically induced bond ruptures and protein unfolding.

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Loading device effect on protein unfolding mechanics

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