Abstract
Phospholipase C-β1 (PLC-β1), one of the PLC-β isozymes, exists as two immunologically distinguishable polypeptides of 150 (PLC-β1a) and 140 kDa (PLC-β1b) which are encoded in two distinct transcripts and generated by alternative splicing of a single gene. In this study, the subcellular localization of the two phospholipases C-β1 proteins was examined in rat C6Bu-1 glioma cells using immunological techniques. Immunoblot analysis revealed that the two forms of PLC-β1 were detectable in both cytosolic and nuclear fractions. PLC-β1a appeared to be located preferentially in the cytosol, whereas PLC-β1b was found predominantly in the nuclei of C6Bu-1 cells. Immunocytochemical experiments confirmed the differential localization of the: two PLC-β1 species in C6Bu-1 cells. These results suggest that the two PLC-β1 proteins may have different physiological roles in the cell.
| Original language | English |
|---|---|
| Pages (from-to) | 76-80 |
| Number of pages | 5 |
| Journal | Biochimica et Biophysica Acta - Lipids and Lipid Metabolism |
| Volume | 1389 |
| Issue number | 1 |
| DOIs | |
| Publication status | Published - 1998 Jan 5 |
Bibliographical note
Funding Information:We would like to thank Ms Gisela Hoschek for proofreading our manuscript. This work was supported in part by the Basic Science Research Institute Program of the Ministry of Education (BSRI 96-4434) and a grant No. KOSEF 961-0100-001-2 from Korea Science and Engineering Foundation.
Keywords
- C6Bu-1 cell
- Immunocytochemistry
- Nuclear localization
- Phospholipase C-β1
ASJC Scopus subject areas
- Biophysics
- Biochemistry
- Endocrinology