Localization of two forms of phospholipase C-β1, a and b, in C6Bu-1 cells

Young Yil Bahk; Hebok Song; Suk Hwan Baek; Byung Yu Park; Hyun Kim; Sung Ho Ryu; Pann Ghill Suh

doi:10.1016/S0005-2760(97)00128-8

Localization of two forms of phospholipase C-β1, a and b, in C6Bu-1 cells

Young Yil Bahk, Hebok Song, Suk Hwan Baek, Byung Yu Park, Hyun Kim, Sung Ho Ryu, Pann Ghill Suh

Department of Biomedical Sciences

Research output: Contribution to journal › Article › peer-review

72 Citations (Scopus)

Abstract

Phospholipase C-β1 (PLC-β1), one of the PLC-β isozymes, exists as two immunologically distinguishable polypeptides of 150 (PLC-β1a) and 140 kDa (PLC-β1b) which are encoded in two distinct transcripts and generated by alternative splicing of a single gene. In this study, the subcellular localization of the two phospholipases C-β1 proteins was examined in rat C6Bu-1 glioma cells using immunological techniques. Immunoblot analysis revealed that the two forms of PLC-β1 were detectable in both cytosolic and nuclear fractions. PLC-β1a appeared to be located preferentially in the cytosol, whereas PLC-β1b was found predominantly in the nuclei of C6Bu-1 cells. Immunocytochemical experiments confirmed the differential localization of the: two PLC-β1 species in C6Bu-1 cells. These results suggest that the two PLC-β1 proteins may have different physiological roles in the cell.

Original language	English
Pages (from-to)	76-80
Number of pages	5
Journal	Biochimica et Biophysica Acta - Lipids and Lipid Metabolism
Volume	1389
Issue number	1
DOIs	https://doi.org/10.1016/S0005-2760(97)00128-8
Publication status	Published - 1998 Jan 5

Keywords

C6Bu-1 cell
Immunocytochemistry
Nuclear localization
Phospholipase C-β1

ASJC Scopus subject areas

Biophysics
Biochemistry
Endocrinology

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10.1016/S0005-2760(97)00128-8

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title = "Localization of two forms of phospholipase C-β1, a and b, in C6Bu-1 cells",

abstract = "Phospholipase C-β1 (PLC-β1), one of the PLC-β isozymes, exists as two immunologically distinguishable polypeptides of 150 (PLC-β1a) and 140 kDa (PLC-β1b) which are encoded in two distinct transcripts and generated by alternative splicing of a single gene. In this study, the subcellular localization of the two phospholipases C-β1 proteins was examined in rat C6Bu-1 glioma cells using immunological techniques. Immunoblot analysis revealed that the two forms of PLC-β1 were detectable in both cytosolic and nuclear fractions. PLC-β1a appeared to be located preferentially in the cytosol, whereas PLC-β1b was found predominantly in the nuclei of C6Bu-1 cells. Immunocytochemical experiments confirmed the differential localization of the: two PLC-β1 species in C6Bu-1 cells. These results suggest that the two PLC-β1 proteins may have different physiological roles in the cell.",

keywords = "C6Bu-1 cell, Immunocytochemistry, Nuclear localization, Phospholipase C-β1",

author = "Bahk, {Young Yil} and Hebok Song and Baek, {Suk Hwan} and Park, {Byung Yu} and Hyun Kim and Ryu, {Sung Ho} and Suh, {Pann Ghill}",

note = "Funding Information: We would like to thank Ms Gisela Hoschek for proofreading our manuscript. This work was supported in part by the Basic Science Research Institute Program of the Ministry of Education (BSRI 96-4434) and a grant No. KOSEF 961-0100-001-2 from Korea Science and Engineering Foundation.",

year = "1998",

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doi = "10.1016/S0005-2760(97)00128-8",

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AU - Bahk, Young Yil

AU - Song, Hebok

AU - Baek, Suk Hwan

AU - Park, Byung Yu

AU - Kim, Hyun

AU - Ryu, Sung Ho

AU - Suh, Pann Ghill

N1 - Funding Information: We would like to thank Ms Gisela Hoschek for proofreading our manuscript. This work was supported in part by the Basic Science Research Institute Program of the Ministry of Education (BSRI 96-4434) and a grant No. KOSEF 961-0100-001-2 from Korea Science and Engineering Foundation.

PY - 1998/1/5

Y1 - 1998/1/5

N2 - Phospholipase C-β1 (PLC-β1), one of the PLC-β isozymes, exists as two immunologically distinguishable polypeptides of 150 (PLC-β1a) and 140 kDa (PLC-β1b) which are encoded in two distinct transcripts and generated by alternative splicing of a single gene. In this study, the subcellular localization of the two phospholipases C-β1 proteins was examined in rat C6Bu-1 glioma cells using immunological techniques. Immunoblot analysis revealed that the two forms of PLC-β1 were detectable in both cytosolic and nuclear fractions. PLC-β1a appeared to be located preferentially in the cytosol, whereas PLC-β1b was found predominantly in the nuclei of C6Bu-1 cells. Immunocytochemical experiments confirmed the differential localization of the: two PLC-β1 species in C6Bu-1 cells. These results suggest that the two PLC-β1 proteins may have different physiological roles in the cell.

AB - Phospholipase C-β1 (PLC-β1), one of the PLC-β isozymes, exists as two immunologically distinguishable polypeptides of 150 (PLC-β1a) and 140 kDa (PLC-β1b) which are encoded in two distinct transcripts and generated by alternative splicing of a single gene. In this study, the subcellular localization of the two phospholipases C-β1 proteins was examined in rat C6Bu-1 glioma cells using immunological techniques. Immunoblot analysis revealed that the two forms of PLC-β1 were detectable in both cytosolic and nuclear fractions. PLC-β1a appeared to be located preferentially in the cytosol, whereas PLC-β1b was found predominantly in the nuclei of C6Bu-1 cells. Immunocytochemical experiments confirmed the differential localization of the: two PLC-β1 species in C6Bu-1 cells. These results suggest that the two PLC-β1 proteins may have different physiological roles in the cell.

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Localization of two forms of phospholipase C-β1, a and b, in C6Bu-1 cells

Abstract

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ASJC Scopus subject areas

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