Localized electroporation effect on adherent cells in modified electric cell-substrate impedance sensing circuits

Yu Jin Kim, Ka Ram Song, Hee Dae Kim, Bum Chul Park, Young Keun Kim, Chi Jung Kang

Research output: Contribution to journalArticlepeer-review

1 Citation (Scopus)

Abstract

Electroporation is a physical transfection method for introducing foreign genes or drugs into cells. It does not require toxic reagents or transfection vectors. However, its applications have been limited because of cell damage and nonspecific transport. Here, we present an effective method for selective and localized electroporation using atomic force microscopy. This electroporation method is applied to adherent cells on substrates, instead of conventionally used suspended cells, and offers relatively effective cell transfection. Moreover, this method enables localized transfection into targeted areas at the single-cell level.

Original languageEnglish
Article number107001
JournalApplied Physics Express
Volume9
Issue number10
DOIs
Publication statusPublished - 2016 Oct

Bibliographical note

Funding Information:
Y.J.K. is grateful for support from the Basic Science Research Program through the National Research Foundation of Korea (NRF) funded by the Ministry of Education (2013R1A1A2063087). Y.K.K. acknowledges support through the National Research Foundation of Korea (2014M3A7B4052193 and 2015R1A2A1A15053002). Y.J.K. acknowledges financial support from Korea University.

Publisher Copyright:
© 2016 The Japan Society of Applied Physics.

ASJC Scopus subject areas

  • General Engineering
  • General Physics and Astronomy

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