Lon domain-containing protein 1 represses thioredoxin y2 and regulates ROS levels in Arabidopsis chloroplasts

Jin Seok Shin, Soo Youn Kim, Won Mi So, Minsoo Noh, Kyoung Shin Yoo, Jeong Sheop Shin

Research output: Contribution to journalArticlepeer-review

2 Citations (Scopus)

Abstract

Plant thioredoxins (Trxs) act as antioxidants and function as redox regulators in the chloroplast. Although the regulation of ROS in chloroplasts is well elucidated, the precise regulation mechanism of Trx remains unknown. Here, we characterize a novel chloroplast protein, Lon domain-containing protein 1 (LCP1), which contains only a Lon domain, the precise function of which is not known. We find that LCP1 interacts with Trx-y2 and represses its activity, and that knockdown (KD) of LCP1 causes anther indehiscence due to deficient lignin deposition. In addition, LCP1 KD plants show less ROS accumulation and lower expression of ROS-responsive marker genes than the wild-type plant. Taken together, we suggest that LCP1 directly regulates Trx-y2 and controls H2O2 levels and, thereby, regulates lignin polymerization in the anther endothecium.

Original languageEnglish
Pages (from-to)986-994
Number of pages9
JournalFEBS Letters
Volume594
Issue number6
DOIs
Publication statusPublished - 2020 Mar 1

Bibliographical note

Funding Information:
This work was supported by two grants (2014R1A2A2A01005974 and 2019R1F1A1060014) from the National Research Foundation of Korea (NRF) funded by the Ministry of Science and by a grant PJ01367001 from the Next‐Generation BioGreen 21 Program funded by the Rural Development Administration, Republic of Korea. This work was also partially supported by Korea University.

Funding Information:
This work was supported by two grants (2014R1A2A2A01005974 and 2019R1F1A1060014) from the National Research Foundation of Korea (NRF) funded by the Ministry of Science and by a grant PJ01367001 from the Next-Generation BioGreen 21 Program funded by the Rural Development Administration, Republic of Korea. This work was also partially supported by Korea University.

Publisher Copyright:
© 2019 Federation of European Biochemical Societies

Keywords

  • Arabidopsis
  • ROS
  • chloroplast
  • dehiscence
  • lignification
  • thioredoxin

ASJC Scopus subject areas

  • Biophysics
  • Structural Biology
  • Biochemistry
  • Molecular Biology
  • Genetics
  • Cell Biology

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