Long-living budding yeast cell subpopulation induced by ethanol/acetate and respiration

Young Yon Kwon, Seung Soo Kim, Han Jun Lee, Seo Hyeong Sheen, Kyoung Heon Kim, Cheol Koo Lee

Research output: Contribution to journalArticlepeer-review

6 Citations (Scopus)


Budding yeast generate heterogeneous cells that can be separated into two distinctive cell types: Short-living low-density and long-living highdensity (HD) cells by density gradient centrifugation. We found that ethanol and acetate induce formation of HD cells, and mitochondrial respiration is required. From their transcriptomes and metabolomes, we found upregulated differentially expressed genes in HD cells involved in the RGT2/RGT1 glucose sensing pathway and its downstream genes encoding hexose transporters. For HD cells, we determined an abundance of various carbon sources including glucose, lactate, pyruvate, trehalose, mannitol, mannose, and galactose. Other upregulated differentially expressed genes in HD cells were involved in the TORC1-SCH9 signaling pathway and its downstream genes involved in cytoplasmic translation. We also measured an abundance of free amino acids in HD cells including valine, proline, isoleucine, and glutamine. These characteristics of the HD cell transcriptome and metabolome may be important conditions for maintaining a long-living phenotype.

Original languageEnglish
Pages (from-to)1448-1456
Number of pages9
JournalJournals of Gerontology - Series A Biological Sciences and Medical Sciences
Issue number8
Publication statusPublished - 2020

Bibliographical note

Funding Information:
This work was supported by a Korea University Grant and carried out with the support of Bio and Medical Technology Development Program through the National Research Foundation of Korea funded by the Ministry of Education, Science and Technology (NRF-2017R1D1A1A02019355).

Publisher Copyright:
© The Author(s) 2019.


  • High-dense cell
  • Longevity
  • Mitochondria
  • Senescence

ASJC Scopus subject areas

  • Ageing
  • Geriatrics and Gerontology


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