Abstract
In this study, we prepared the platelet-derived growth factor-containing porous microspheres modified with heparin (PDGF/Hep-PMSs) and investigated their anti-inflammatory and tendon healing effects on rotator cuff (RC) tendinitis rabbit model. PDGF/Hep-PMSs suppressed the mRNA levels of six pro-inflammatory cytokines (i.e., MMP-3, MMP-13, COX-2, ADAMTS-5, IL-6, and TNF-α) in inflamed tenocytes. Long-term local delivery of PDGF/Hep-PMSs into tendon tissues of RC tendinitis decreased the mRNA levels of six pro-inflammatory cytokines and increased the mRNA levels of anti-inflammatory cytokines including IL-4, IL-10, and IL-13. Anti-inflammatory effects of PDGF/Hep-PMSs might have contributed to enhance the collagen content, tenogenic markers, stiffness, and tensile strength of tendons, eventually leading to tendon restoration. Our findings suggest that the long-term local PDGF delivery of PDGF/Hep-PMSs have a great potential to enhance tendon healing of RC tendinitis by suppressing inflammation responses.
Original language | English |
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Pages (from-to) | 372-381 |
Number of pages | 10 |
Journal | Carbohydrate Polymers |
Volume | 209 |
DOIs | |
Publication status | Published - 2019 Apr 1 |
Bibliographical note
Funding Information:This work is funded by a Korea University Guro Hospital Grant ( O1600151 ) and grants from the Korea Health Technology R&D Project, Ministry of Health and Welfare, Republic of Korea ( HI13C1501 and HI15C1665 ).
Publisher Copyright:
© 2019 Elsevier Ltd
Keywords
- 2-(N-morpholino)-ethanesulfonic acid (Pubchem CID:4478249)
- Anti-inflammation
- Dichloromethane (DCM) (PubChem CID: 6344)
- Dimethylsulfoxide (DMSO) (PubChem CID: 679)
- Dopamine hydrochloride (PubChem CID:65340)
- Heparin
- Heparin sodium (PubChem CID:22833565)
- PDGF
- Poly (lactic-co-glycolic acid) (PLGA) (Pubchem CID: 23111554)
- Polyvinyl alcohol (PVA) (PubChem CID: 11199)
- Porous microspheres
- Recombinant human PDGF (PubChem SID: 135290697)
- Rotator cuff tendinitis
- Sodium chloride (PubChem CID:5234)
- Tendon healing
- Thiazolyl blue tetrazolium bromide (MTT) (PubChem CID:64965)
ASJC Scopus subject areas
- Materials Chemistry
- Polymers and Plastics
- Organic Chemistry