The standard method for the storage and preservation of RNA has been at ultra-low temperatures. However, reliance on liquid nitrogen and freezers for storage of RNA has multiple downsides. Recently new techniques have been developed for storing RNA at room temperature utilizing desiccation and are reported to be an effective alternative for preserving RNA integrity. In this study we compared frozen RNA samples stored for up to one year to those which had been desiccated using RNAstable (Biomatrica, Inc., San Diego, CA) and stored at room temperature. RNA samples were placed in aliquots and stored after desiccation or frozen (at 280°C), and were analyzed for RNA Integrity Number (RIN), and by qPCR, and RNA sequencing. Our study shows that RNAstable is able to preserve desiccated RNA samples at room temperature for up to one year, and that RNA preserved by desiccation is comparable to cryopreserved RNA for downstream analyses including realtime-PCR and RNA sequencing.
Bibliographical noteFunding Information:
The authors acknowledge support from Biomatrica, Inc., San Diego, CA for providing supplies, Dave Turnquist, Assistant Vice Chancellor for Facilities Operations at the University of Colorado Anschutz Medical Campus for funding, and the Moore family foundation for their support.
© 2014 PLOS ONE.
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