Low-blinking SERS substrate for switchable detection of kanamycin

Anh H. Nguyen, Xingyi Ma, Hyun Gyu Park, Sang Jun Sim

    Research output: Contribution to journalArticlepeer-review

    63 Citations (Scopus)

    Abstract

    Metallic plasmonic nanoparticles generate surface-enhanced Raman Scattering (SERS) for wide sensing applications of biomolecules, gas and environmental contaminants. However, the metallic materials cause high thermal radiation during laser excitation in the opto-microfluidic systems and give rise to temporal intensity fluctuations, which is called blinking effects in SERS spectra. This work developed nanocomposite consisting of graphene oxide (GO) and gold nanoparticles (AuNPs) with high thermal stability and low thermal radiation, which provided plasmonic resonance while reduced blinking effect for SERS-based sensing in the microfluidic detection system. The system was applied to detect kanamycin in drinking water, orange juice, and milk by using Texas Red-dye conjugated Beacon aptamer, where the aptamer was designed to be specific and switchable in kanamycin recognition. The sensor was reusable, and its limit of detection was 0.75 nM in a linear range of 1 nM to 100 nM. The microfluidic SERS detection system can be potentially used for sensitive and reliable assays of small molecules.

    Original languageEnglish
    Pages (from-to)765-773
    Number of pages9
    JournalSensors and Actuators, B: Chemical
    Volume282
    DOIs
    Publication statusPublished - 2019 Mar 1

    Bibliographical note

    Funding Information:
    This study was supported by the National Research Foundation (NRF) grant ( 2016R1A2A1A05005465/2010–0027955/2018R1A2A1A05022355 ) and the grants ( 2014M1A8A1049278 ) from Korea Carbon Capture and Sequestration (CCS) R&D Centre of the National Research Foundation (NRF) of the Ministry of Science, ICT, and Future Planning of Korea . X.M. was supported by a Research Professor program ( 141594 ) of Korea University.

    Funding Information:
    This study was supported by the National Research Foundation (NRF) grant (2016R1A2A1A05005465/2010–0027955/2018R1A2A1A05022355) and the grants (2014M1A8A1049278) from Korea Carbon Capture and Sequestration (CCS) R&D Centre of the National Research Foundation (NRF) of the Ministry of Science, ICT, and Future Planning of Korea. X.M. was supported by a Research Professor program (141594) of Korea University.

    Publisher Copyright:
    © 2018 Elsevier B.V.

    Keywords

    • Antibiotic sensor
    • Blinking effects
    • Gold nanoparticles
    • Graphene oxide
    • SERS

    ASJC Scopus subject areas

    • Electronic, Optical and Magnetic Materials
    • Instrumentation
    • Condensed Matter Physics
    • Surfaces, Coatings and Films
    • Metals and Alloys
    • Electrical and Electronic Engineering
    • Materials Chemistry

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