Lysine-specific demethylase 3A is important for autophagic occurrence

Joomyung Kim; Seon Ah Choi; Jaebeom Kim; Hyunkyung Kim; Sung Hee Baek

doi:10.1016/j.bbrc.2020.03.058

Lysine-specific demethylase 3A is important for autophagic occurrence

Joomyung Kim, Seon Ah Choi, Jaebeom Kim, Hyunkyung Kim, Sung Hee Baek

Department of Biomedical Sciences

Research output: Contribution to journal › Article › peer-review

4 Citations (Scopus)

Abstract

Autophagy is an essential process to maintain cell survival and homeostasis under various stress conditions. Here, we report that lysine-specific demethylase 3A (KDM3A) plays an important role in starvation-induced autophagy. Using Kdm3a knockout mice, we demonstrate that KDM3A is crucial for proper hepatic autophagy in vivo. Hepatic mRNA expression analysis and ChIP assay in WT and Kdm3a knockout mouse livers reveal that KDM3A activates autophagy genes by reducing histone H3K9me2 levels upon fasting. Together, our finding represents previously unidentified function of KDM3A as a key regulator of autophagy, implicating potential therapeutic approaches for autophagy-related diseases.

Original language	English
Pages (from-to)	176-183
Number of pages	8
Journal	Biochemical and biophysical research communications
Volume	526
Issue number	1
DOIs	https://doi.org/10.1016/j.bbrc.2020.03.058
Publication status	Published - 2020 May 21

Bibliographical note

Funding Information:
This work was supported by Creative Research Initiatives Program (Research Center for Epigenetic Code and Diseases , 2017R1A3B1023387 ) and the Science Research Center program ( NRF-2016R1A5A1010764 ) to S.H.B.; Global PhD Fellowship Program ( NRF-2019H1A2A1073581 ) to S.A.C.; the Basic Science Research Program ( NRF-2014R1A6A3A04057910 ) and Korea University Grant ( K1925011 ) to H.K. from the National Research Foundation ( NRF ) grant funded by the Korea government.

Funding Information:
This work was supported by Creative Research Initiatives Program (Research Center for Epigenetic Code and Diseases, 2017R1A3B1023387) and the Science Research Center program (NRF-2016R1A5A1010764) to S.H.B.; Global PhD Fellowship Program (NRF-2019H1A2A1073581) to S.A.C.; the Basic Science Research Program (NRF-2014R1A6A3A04057910) and Korea University Grant (K1925011) to H.K. from the National Research Foundation (NRF) grant funded by the Korea government.

Publisher Copyright:
© 2020 Elsevier Inc.

Keywords

Autophagy
H3K9 demethylation
KDM3A

ASJC Scopus subject areas

Biophysics
Biochemistry
Molecular Biology
Cell Biology

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10.1016/j.bbrc.2020.03.058

Cite this

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title = "Lysine-specific demethylase 3A is important for autophagic occurrence",

abstract = "Autophagy is an essential process to maintain cell survival and homeostasis under various stress conditions. Here, we report that lysine-specific demethylase 3A (KDM3A) plays an important role in starvation-induced autophagy. Using Kdm3a knockout mice, we demonstrate that KDM3A is crucial for proper hepatic autophagy in vivo. Hepatic mRNA expression analysis and ChIP assay in WT and Kdm3a knockout mouse livers reveal that KDM3A activates autophagy genes by reducing histone H3K9me2 levels upon fasting. Together, our finding represents previously unidentified function of KDM3A as a key regulator of autophagy, implicating potential therapeutic approaches for autophagy-related diseases.",

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author = "Joomyung Kim and Choi, {Seon Ah} and Jaebeom Kim and Hyunkyung Kim and Baek, {Sung Hee}",

note = "Funding Information: This work was supported by Creative Research Initiatives Program (Research Center for Epigenetic Code and Diseases , 2017R1A3B1023387 ) and the Science Research Center program ( NRF-2016R1A5A1010764 ) to S.H.B.; Global PhD Fellowship Program ( NRF-2019H1A2A1073581 ) to S.A.C.; the Basic Science Research Program ( NRF-2014R1A6A3A04057910 ) and Korea University Grant ( K1925011 ) to H.K. from the National Research Foundation ( NRF ) grant funded by the Korea government. Funding Information: This work was supported by Creative Research Initiatives Program (Research Center for Epigenetic Code and Diseases, 2017R1A3B1023387) and the Science Research Center program (NRF-2016R1A5A1010764) to S.H.B.; Global PhD Fellowship Program (NRF-2019H1A2A1073581) to S.A.C.; the Basic Science Research Program (NRF-2014R1A6A3A04057910) and Korea University Grant (K1925011) to H.K. from the National Research Foundation (NRF) grant funded by the Korea government. Publisher Copyright: {\textcopyright} 2020 Elsevier Inc.",

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doi = "10.1016/j.bbrc.2020.03.058",

language = "English",

volume = "526",

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journal = "Biochemical and biophysical research communications",

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AU - Kim, Hyunkyung

AU - Baek, Sung Hee

N1 - Funding Information: This work was supported by Creative Research Initiatives Program (Research Center for Epigenetic Code and Diseases , 2017R1A3B1023387 ) and the Science Research Center program ( NRF-2016R1A5A1010764 ) to S.H.B.; Global PhD Fellowship Program ( NRF-2019H1A2A1073581 ) to S.A.C.; the Basic Science Research Program ( NRF-2014R1A6A3A04057910 ) and Korea University Grant ( K1925011 ) to H.K. from the National Research Foundation ( NRF ) grant funded by the Korea government. Funding Information: This work was supported by Creative Research Initiatives Program (Research Center for Epigenetic Code and Diseases, 2017R1A3B1023387) and the Science Research Center program (NRF-2016R1A5A1010764) to S.H.B.; Global PhD Fellowship Program (NRF-2019H1A2A1073581) to S.A.C.; the Basic Science Research Program (NRF-2014R1A6A3A04057910) and Korea University Grant (K1925011) to H.K. from the National Research Foundation (NRF) grant funded by the Korea government. Publisher Copyright: © 2020 Elsevier Inc.

PY - 2020/5/21

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N2 - Autophagy is an essential process to maintain cell survival and homeostasis under various stress conditions. Here, we report that lysine-specific demethylase 3A (KDM3A) plays an important role in starvation-induced autophagy. Using Kdm3a knockout mice, we demonstrate that KDM3A is crucial for proper hepatic autophagy in vivo. Hepatic mRNA expression analysis and ChIP assay in WT and Kdm3a knockout mouse livers reveal that KDM3A activates autophagy genes by reducing histone H3K9me2 levels upon fasting. Together, our finding represents previously unidentified function of KDM3A as a key regulator of autophagy, implicating potential therapeutic approaches for autophagy-related diseases.

AB - Autophagy is an essential process to maintain cell survival and homeostasis under various stress conditions. Here, we report that lysine-specific demethylase 3A (KDM3A) plays an important role in starvation-induced autophagy. Using Kdm3a knockout mice, we demonstrate that KDM3A is crucial for proper hepatic autophagy in vivo. Hepatic mRNA expression analysis and ChIP assay in WT and Kdm3a knockout mouse livers reveal that KDM3A activates autophagy genes by reducing histone H3K9me2 levels upon fasting. Together, our finding represents previously unidentified function of KDM3A as a key regulator of autophagy, implicating potential therapeutic approaches for autophagy-related diseases.

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Lysine-specific demethylase 3A is important for autophagic occurrence

Abstract

Bibliographical note

Keywords

ASJC Scopus subject areas

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