Macromolecule delivery into hard-to-transefct primary cells via hydrodynamic cell deformation

Jeongsoo Hur, Aram Chung

    Research output: Chapter in Book/Report/Conference proceedingConference contribution

    Abstract

    We present a versatile microfluidic intracellular delivery platform that can deliver large nanomaterials effectively into diverse primary cells via hydrodynamic cell deformation in a novel T-junction microchannel with a cavity. Briefly, a syringe pump was used to inject a cell suspension with cargo of interest into a microchannel at a moderate Re to induce the development of inertial vortices. The recirculating flows deformed the cells, generating transient nanopores on the cellular membrane. This intrinsically cell deformation enables highly effective transport of different nanomaterials into various cell types including difficult-to-transfect primary cells, in a high-throughput and minimally invasive manner.

    Original languageEnglish
    Title of host publicationMicroTAS 2020 - 24th International Conference on Miniaturized Systems for Chemistry and Life Sciences
    PublisherChemical and Biological Microsystems Society
    Pages817-818
    Number of pages2
    ISBN (Electronic)9781733419017
    Publication statusPublished - 2020
    Event24th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2020 - Virtual, Online
    Duration: 2020 Oct 42020 Oct 9

    Publication series

    NameMicroTAS 2020 - 24th International Conference on Miniaturized Systems for Chemistry and Life Sciences

    Conference

    Conference24th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2020
    CityVirtual, Online
    Period20/10/420/10/9

    Bibliographical note

    Funding Information:
    This work is supported by the Samsung Research Funding and Incubation Center for Future Technology (Grant No. SRFC-IT1802-03).

    Publisher Copyright:
    © 2020 CBMS-0001

    Keywords

    • Cell Transfection
    • Cell-based Therapy
    • Gene Delivery
    • Hydroporator
    • Intracellular delivery

    ASJC Scopus subject areas

    • Chemical Engineering (miscellaneous)
    • Bioengineering
    • General Chemistry
    • Control and Systems Engineering

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