Abstract
We present a versatile microfluidic intracellular delivery platform that can deliver large nanomaterials effectively into diverse primary cells via hydrodynamic cell deformation in a novel T-junction microchannel with a cavity. Briefly, a syringe pump was used to inject a cell suspension with cargo of interest into a microchannel at a moderate Re to induce the development of inertial vortices. The recirculating flows deformed the cells, generating transient nanopores on the cellular membrane. This intrinsically cell deformation enables highly effective transport of different nanomaterials into various cell types including difficult-to-transfect primary cells, in a high-throughput and minimally invasive manner.
Original language | English |
---|---|
Title of host publication | MicroTAS 2020 - 24th International Conference on Miniaturized Systems for Chemistry and Life Sciences |
Publisher | Chemical and Biological Microsystems Society |
Pages | 817-818 |
Number of pages | 2 |
ISBN (Electronic) | 9781733419017 |
Publication status | Published - 2020 |
Event | 24th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2020 - Virtual, Online Duration: 2020 Oct 4 → 2020 Oct 9 |
Publication series
Name | MicroTAS 2020 - 24th International Conference on Miniaturized Systems for Chemistry and Life Sciences |
---|
Conference
Conference | 24th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2020 |
---|---|
City | Virtual, Online |
Period | 20/10/4 → 20/10/9 |
Bibliographical note
Funding Information:This work is supported by the Samsung Research Funding and Incubation Center for Future Technology (Grant No. SRFC-IT1802-03).
Publisher Copyright:
© 2020 CBMS-0001
Keywords
- Cell Transfection
- Cell-based Therapy
- Gene Delivery
- Hydroporator
- Intracellular delivery
ASJC Scopus subject areas
- Chemical Engineering (miscellaneous)
- Bioengineering
- General Chemistry
- Control and Systems Engineering