Abstract
A novel, two-photon probe for the detection of free Mg2+ ions in living cells and live tissues has been developed. The probe can be excited by 880 nm laser photons, emits strong two-photon excited fluorescence in response to Mg2+ ions, can be easily loaded into the cell and tissue, shows high photostability, and can measure the Mg2+ ion concentration without interference by Ca2+ ions in living cells. The intracellular dissociation constant (Kdi) for Mg2+ determined by the two-photon process is 2.5 mM, which is suitable for dynamic Mg 2+ concentration measurement. In addition, the probe is capable of imaging endogenous stores of free Mg2+ at a few hundred micrometers depth in live tissues using two-photon microscopy (TPM).
Original language | English |
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Pages (from-to) | 2088-2096 |
Number of pages | 9 |
Journal | Journal of Organic Chemistry |
Volume | 72 |
Issue number | 6 |
DOIs | |
Publication status | Published - 2007 Mar 16 |
ASJC Scopus subject areas
- Organic Chemistry