Magnesium ion selective two-photon fluorescent probe based on a benzo[h]chromene derivative for in vivo imaging

Hwan Myung Kim; Pil Rye Yang; Mun Sik Seo; Jae Sung Yi; Jin Hee Hong; Seung Joon Jeon; Young Gyu Ko; Kyoung J. Lee; Bong Rae Cho

doi:10.1021/jo062341m

Magnesium ion selective two-photon fluorescent probe based on a benzo[h]chromene derivative for in vivo imaging

Hwan Myung Kim
, Pil Rye Yang
, Mun Sik Seo
, Jae Sung Yi
, Jin Hee Hong
, Seung Joon Jeon
, Young Gyu Ko
, Kyoung J. Lee
, Bong Rae Cho^*

^*Corresponding author for this work

Research output: Contribution to journal › Article › peer-review

139 Citations (Scopus)

Abstract

A novel, two-photon probe for the detection of free Mg²⁺ ions in living cells and live tissues has been developed. The probe can be excited by 880 nm laser photons, emits strong two-photon excited fluorescence in response to Mg²⁺ ions, can be easily loaded into the cell and tissue, shows high photostability, and can measure the Mg²⁺ ion concentration without interference by Ca²⁺ ions in living cells. The intracellular dissociation constant (K_dⁱ) for Mg²⁺ determined by the two-photon process is 2.5 mM, which is suitable for dynamic Mg ²⁺ concentration measurement. In addition, the probe is capable of imaging endogenous stores of free Mg²⁺ at a few hundred micrometers depth in live tissues using two-photon microscopy (TPM).

Original language	English
Pages (from-to)	2088-2096
Number of pages	9
Journal	Journal of Organic Chemistry
Volume	72
Issue number	6
DOIs	https://doi.org/10.1021/jo062341m
Publication status	Published - 2007 Mar 16

ASJC Scopus subject areas

Organic Chemistry

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10.1021/jo062341m

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title = "Magnesium ion selective two-photon fluorescent probe based on a benzo[h]chromene derivative for in vivo imaging",

abstract = "A novel, two-photon probe for the detection of free Mg2+ ions in living cells and live tissues has been developed. The probe can be excited by 880 nm laser photons, emits strong two-photon excited fluorescence in response to Mg2+ ions, can be easily loaded into the cell and tissue, shows high photostability, and can measure the Mg2+ ion concentration without interference by Ca2+ ions in living cells. The intracellular dissociation constant (Kdi) for Mg2+ determined by the two-photon process is 2.5 mM, which is suitable for dynamic Mg 2+ concentration measurement. In addition, the probe is capable of imaging endogenous stores of free Mg2+ at a few hundred micrometers depth in live tissues using two-photon microscopy (TPM).",

author = "Kim, \{Hwan Myung\} and Yang, \{Pil Rye\} and Seo, \{Mun Sik\} and Yi, \{Jae Sung\} and Hong, \{Jin Hee\} and Jeon, \{Seung Joon\} and Ko, \{Young Gyu\} and Lee, \{Kyoung J.\} and Cho, \{Bong Rae\}",

year = "2007",

month = mar,

day = "16",

doi = "10.1021/jo062341m",

language = "English",

volume = "72",

pages = "2088--2096",

journal = "Journal of Organic Chemistry",

issn = "0022-3263",

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TY - JOUR

T1 - Magnesium ion selective two-photon fluorescent probe based on a benzo[h]chromene derivative for in vivo imaging

AU - Kim, Hwan Myung

AU - Yang, Pil Rye

AU - Seo, Mun Sik

AU - Yi, Jae Sung

AU - Hong, Jin Hee

AU - Jeon, Seung Joon

AU - Ko, Young Gyu

AU - Lee, Kyoung J.

AU - Cho, Bong Rae

PY - 2007/3/16

Y1 - 2007/3/16

N2 - A novel, two-photon probe for the detection of free Mg2+ ions in living cells and live tissues has been developed. The probe can be excited by 880 nm laser photons, emits strong two-photon excited fluorescence in response to Mg2+ ions, can be easily loaded into the cell and tissue, shows high photostability, and can measure the Mg2+ ion concentration without interference by Ca2+ ions in living cells. The intracellular dissociation constant (Kdi) for Mg2+ determined by the two-photon process is 2.5 mM, which is suitable for dynamic Mg 2+ concentration measurement. In addition, the probe is capable of imaging endogenous stores of free Mg2+ at a few hundred micrometers depth in live tissues using two-photon microscopy (TPM).

AB - A novel, two-photon probe for the detection of free Mg2+ ions in living cells and live tissues has been developed. The probe can be excited by 880 nm laser photons, emits strong two-photon excited fluorescence in response to Mg2+ ions, can be easily loaded into the cell and tissue, shows high photostability, and can measure the Mg2+ ion concentration without interference by Ca2+ ions in living cells. The intracellular dissociation constant (Kdi) for Mg2+ determined by the two-photon process is 2.5 mM, which is suitable for dynamic Mg 2+ concentration measurement. In addition, the probe is capable of imaging endogenous stores of free Mg2+ at a few hundred micrometers depth in live tissues using two-photon microscopy (TPM).

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U2 - 10.1021/jo062341m

DO - 10.1021/jo062341m

M3 - Article

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AN - SCOPUS:33947226944

SN - 0022-3263

VL - 72

SP - 2088

EP - 2096

JO - Journal of Organic Chemistry

JF - Journal of Organic Chemistry

IS - 6

ER -

Magnesium ion selective two-photon fluorescent probe based on a benzo[h]chromene derivative for in vivo imaging

Abstract

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