Magnetic-Fluorescent Nanocluster Lateral Flow Assay for Rotavirus Detection

Hong En Fu, Thomas Myeongseok Koo, Myeong Soo Kim, Min Jun Ko, Bum Chul Park, Kyuha Oh, Younhyung Cho, Jae Wan Jung, Sungil Kim, Woong Sik Jang, Chae Seung Lim, Young Keun Kim

Research output: Contribution to journalArticlepeer-review

4 Citations (Scopus)

Abstract

Rotavirus is a leading cause of death in young children worldwide. Although vaccines are currently available and reduce the disease burden, most low-income countries rely on diagnosis by point-of-care testing owing to its rapidity, simplicity, and affordability; however, its sensitivity remains a problem. Magnetically assisted antigen enrichment improves the sensitivity with increased fluorescence intensity. However, magnetic nanoparticles (NPs) strongly absorb ultraviolet light, limiting the combination of magnetic NPs and quantum dots (QDs). Therefore, this study aimed to establish a magnetic-fluorescent nanocluster composed of magnetite (Fe3O4) NPs and CdSe-CdS core-shell QDs for a high-sensitivity lateral flow assay (LFA). Inducing a solvophobic interaction mediated by solvent polarity increased the interparticle distance. Consequently, the self-assembled nanoclusters exhibited a QD-embedded structure with Fe3O4 NPs on the outer layer. The magnetic enrichment enhanced the detection limit of the rotavirus antigen up to 1.0 × 101 TCID50/mL, where TCID50 represents the median tissue culture infectious dose. The clinical trial results showed that the established LFA platform outperformed commercial test kits. Thus, this study provides a nanotechnology-based LFA platform with increased sensitivity for inhibiting the propagation of viruses.

Original languageEnglish
Pages (from-to)5789-5798
Number of pages10
JournalACS Applied Nano Materials
Volume6
Issue number7
DOIs
Publication statusPublished - 2023 Apr 14

Bibliographical note

Publisher Copyright:
© 2023 American Chemical Society.

Keywords

  • lateral flow assay
  • magnetic nanocluster
  • quantum dot
  • rotavirus
  • solvophobic interaction

ASJC Scopus subject areas

  • General Materials Science

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