Magnetic Nanoparticles-Embedded Enzyme-Inorganic Hybrid Nanoflowers with Enhanced Peroxidase-Like Activity and Substrate Channeling for Glucose Biosensing

Hong Jae Cheon, Manab Deb Adhikari, Minsoo Chung, Tai Duc Tran, Jungbae Kim, Moon Il Kim

Research output: Contribution to journalArticlepeer-review

80 Citations (Scopus)

Abstract

It is reported that glucose oxidase (GOx)-copper hybrid nanoflowers embedded with Fe3O4 magnetic nanoparticles (MNPs) exhibit superior peroxidase-mimicking activity as well as substrate channeling for glucose detection. This is due to the synergistic integration of GOx, crystalline copper phosphates and MNPs being in close proximity within the nanoflowers. The preparation of MNP-embedded GOx-copper hybrid nanoflowers (MNPs-GOx NFs) begins with the facile conjugation of amine-functionalized MNPs with GOx molecules via electrostatic attraction, followed by the addition of copper sulfate that leads to full blooming of the hybrid nanoflowers. In the presence of glucose, the catalytic action of GOx entrapped in the nanoflowers generates H2O2, which is subsequently used by peroxidase-mimicking MNPs and copper phosphate crystals, located close to GOx molecules, to convert Amplex UltraRed substrate into a highly fluorescent product. Using this strategy, the target glucose is successfully determined with excellent selectivity, stability, and magnetic reusability. This biosensor based on hybrid nanoflowers also exhibits a high degree of precision and reproducibility when applied to real human blood samples. Such novel MNP-embedded enzyme-inorganic hybrid nanoflowers have a great potential to be expanded to any oxidases, which will be highly beneficial for the detection of various other clinically important target molecules.

Original languageEnglish
Article number1801507
JournalAdvanced Healthcare Materials
Volume8
Issue number9
DOIs
Publication statusPublished - 2019 May 9

Bibliographical note

Funding Information:
This work was supported by the National Research Foundation of Korea (NRF) grant funded by the Korea government [Ministry of Science and ICT (NRF- 2017R1C1B2009460)]. This work was also supported by the Global Research Laboratory Program (2014K1A1A2043032) and Nano-Material Technology Development Program (2014M3A7B4052193) through the National Research Foundation of Korea (NRF) grants funded by the Korea government Ministry of Science and ICT.

Funding Information:
This work was supported by the National Research Foundation of Korea (NRF) grant funded by the Korea government [Ministry of Science and ICT (NRF-2017R1C1B2009460)]. This work was also supported by the Global Research Laboratory Program (2014K1A1A2043032) and Nano-Material Technology Development Program (2014M3A7B4052193) through the National Research Foundation of Korea (NRF) grants funded by the Korea government Ministry of Science and ICT.

Keywords

  • enzyme-inorganic hybrid nanoflowers
  • glucose detection
  • magnetic nanoparticles
  • peroxidase-like activity
  • substrate channeling

ASJC Scopus subject areas

  • Biomaterials
  • Biomedical Engineering
  • Pharmaceutical Science

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