TY - JOUR
T1 - Maltotetraose Production Using Pseudomonas stutzeri Exo‐α‐Amylase in a Membrane Recycle Bioreactor
AU - Woo, Gun-Jo
AU - McCORD, J. D.
PY - 1991
Y1 - 1991
N2 - For a continuous process using maltotetraose (G4)‐forming amylase in a membrane recycle bioreactor (MRB), the enzyme was partially purified to specific activity 21.5 IU/mg protein. The enzyme was stable enough to convert starch into G4 in batch and MRB systems. Optimum conditions in the MRB using hollow fiber with 1,000 molecular weight cut‐off were: substrate concentration 1.0% (w/v), enzyme concentration 70 IU/L reactant, and residence time 83 min. Productivity at steady state under optimized conditions was 0.99 g/L/hr. Excessive residence time decreased product purity by further conversion of G4 to lower molecular weight compounds due to endo‐acting activity of the enzyme.
AB - For a continuous process using maltotetraose (G4)‐forming amylase in a membrane recycle bioreactor (MRB), the enzyme was partially purified to specific activity 21.5 IU/mg protein. The enzyme was stable enough to convert starch into G4 in batch and MRB systems. Optimum conditions in the MRB using hollow fiber with 1,000 molecular weight cut‐off were: substrate concentration 1.0% (w/v), enzyme concentration 70 IU/L reactant, and residence time 83 min. Productivity at steady state under optimized conditions was 0.99 g/L/hr. Excessive residence time decreased product purity by further conversion of G4 to lower molecular weight compounds due to endo‐acting activity of the enzyme.
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U2 - 10.1111/j.1365-2621.1991.tb14631.x
DO - 10.1111/j.1365-2621.1991.tb14631.x
M3 - Article
AN - SCOPUS:84985200971
SN - 0022-1147
VL - 56
SP - 1019
EP - 1023
JO - Journal of Food Science
JF - Journal of Food Science
IS - 4
ER -