Manipulation of cells using an ultrasonic pressure field

Albrecht Haake; Adrian Neild; Deok Ho Kim; Jong Eun Ihm; Yu Sun; Jürg Dual; Byeong Kwon Ju

doi:10.1016/j.ultrasmedbio.2005.03.004

Manipulation of cells using an ultrasonic pressure field

Albrecht Haake, Adrian Neild, Deok Ho Kim, Jong Eun Ihm, Yu Sun, Jürg Dual, Byeong Kwon Ju

Research output: Contribution to journal › Article › peer-review

58 Citations (Scopus)

Abstract

A novel device is described that generates an ultrasonic force field in a fluid layer. The force field arises because of the acoustic radiation force, a second order effect, generated as an ultrasonic wave interacts with a suspended particle. This force field can be used to manipulate objects in the fluid layer trapped between this device and an arbitrary surface, in this case, a flat object slide. The device is shown to be capable of positioning and, in doing so, concentrating human cells to predictable locations. Mesenchymal and HeLa cells were used. Critically, the forces required to do this can be generated by ultrasonic pressure fields that do not affect the viability of the cells. The viability has been assessed using trypan blue dye. The device used consists of a 14 mm square glass plate that is excited by at least one of four piezotransducers attached to the edges. The resulting ultrasonic force field and, importantly, the location of the minima in the force potential at which the cells are collected, has been calculated analytically.

Original language	English
Pages (from-to)	857-864
Number of pages	8
Journal	Ultrasound in Medicine and Biology
Volume	31
Issue number	6
DOIs	https://doi.org/10.1016/j.ultrasmedbio.2005.03.004
Publication status	Published - 2005 Jun
Externally published	Yes

Bibliographical note

Funding Information:
This work was supported by KTI/CTI, Switzerland, under a Top Nano 21 grant (project numbers 6643.1 and 6989.1). In addition, Deok-Ho Kim was supported by the International Research Internship Program of the Korea Science and Engineering Foundation (KOSEF) (contract number M07-2003-000-10015-0) and by the 21st Century’s Frontier R&D Projects, sponsored by the Ministry of Science and Technology, Korea. The authors thank Jeffrey A. Hubbell, Institute for Biologic Engineering and Biotechnology, Swiss Federal Institute of Technology, Lausanne, Switzerland, and Brad Nelson, Institute of Robots and Intelligent Systems, Swiss Federal Institute of Technology, Zurich, Switzerland, for their encouragement and comments regarding this work.

Keywords

Lamb wave
Manipulation
Positioning
Standing wave

ASJC Scopus subject areas

Radiological and Ultrasound Technology
Biophysics
Acoustics and Ultrasonics

Access to Document

10.1016/j.ultrasmedbio.2005.03.004

Cite this

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title = "Manipulation of cells using an ultrasonic pressure field",

abstract = "A novel device is described that generates an ultrasonic force field in a fluid layer. The force field arises because of the acoustic radiation force, a second order effect, generated as an ultrasonic wave interacts with a suspended particle. This force field can be used to manipulate objects in the fluid layer trapped between this device and an arbitrary surface, in this case, a flat object slide. The device is shown to be capable of positioning and, in doing so, concentrating human cells to predictable locations. Mesenchymal and HeLa cells were used. Critically, the forces required to do this can be generated by ultrasonic pressure fields that do not affect the viability of the cells. The viability has been assessed using trypan blue dye. The device used consists of a 14 mm square glass plate that is excited by at least one of four piezotransducers attached to the edges. The resulting ultrasonic force field and, importantly, the location of the minima in the force potential at which the cells are collected, has been calculated analytically.",

keywords = "Lamb wave, Manipulation, Positioning, Standing wave",

author = "Albrecht Haake and Adrian Neild and Kim, {Deok Ho} and Ihm, {Jong Eun} and Yu Sun and J{\"u}rg Dual and Ju, {Byeong Kwon}",

note = "Funding Information: This work was supported by KTI/CTI, Switzerland, under a Top Nano 21 grant (project numbers 6643.1 and 6989.1). In addition, Deok-Ho Kim was supported by the International Research Internship Program of the Korea Science and Engineering Foundation (KOSEF) (contract number M07-2003-000-10015-0) and by the 21st Century{\textquoteright}s Frontier R&D Projects, sponsored by the Ministry of Science and Technology, Korea. The authors thank Jeffrey A. Hubbell, Institute for Biologic Engineering and Biotechnology, Swiss Federal Institute of Technology, Lausanne, Switzerland, and Brad Nelson, Institute of Robots and Intelligent Systems, Swiss Federal Institute of Technology, Zurich, Switzerland, for their encouragement and comments regarding this work. ",

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AU - Haake, Albrecht

AU - Neild, Adrian

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AU - Ihm, Jong Eun

AU - Sun, Yu

AU - Dual, Jürg

AU - Ju, Byeong Kwon

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PY - 2005/6

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N2 - A novel device is described that generates an ultrasonic force field in a fluid layer. The force field arises because of the acoustic radiation force, a second order effect, generated as an ultrasonic wave interacts with a suspended particle. This force field can be used to manipulate objects in the fluid layer trapped between this device and an arbitrary surface, in this case, a flat object slide. The device is shown to be capable of positioning and, in doing so, concentrating human cells to predictable locations. Mesenchymal and HeLa cells were used. Critically, the forces required to do this can be generated by ultrasonic pressure fields that do not affect the viability of the cells. The viability has been assessed using trypan blue dye. The device used consists of a 14 mm square glass plate that is excited by at least one of four piezotransducers attached to the edges. The resulting ultrasonic force field and, importantly, the location of the minima in the force potential at which the cells are collected, has been calculated analytically.

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Manipulation of cells using an ultrasonic pressure field

Abstract

Bibliographical note

Keywords

ASJC Scopus subject areas

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