Manipulation of the genes for poly-β-hydroxybutyric acid synthesis in Alcaligenes eutrophus

Hae Chul Park; Jin Seo Park; Yong Hyun Lee; Tae Lin Huh

doi:10.1007/BF00130359

Manipulation of the genes for poly-β-hydroxybutyric acid synthesis in Alcaligenes eutrophus

Hae Chul Park, Jin Seo Park, Yong Hyun Lee, Tae Lin Huh

Research output: Contribution to journal › Article › peer-review

1 Citation (Scopus)

Abstract

In order to establish the molecular breeding system in Alcaligenes eutrophus producing poly-β-hydroxybutyric acid (PHB), phbCAB genes from A. eutrophus were recombined into the E. coli-A. eutrophus shuttle vector and directly transferred into A. eutrophus by the electroporation. In A. eutrophus transformants, recombinant plasmids were stably maintained and enzyme activities for PHB biosyntheses were elevated 1.4-2.7 fold by the cloned genes.

Original language	English
Pages (from-to)	729-734
Number of pages	6
Journal	Biotechnology letters
Volume	17
Issue number	7
DOIs	https://doi.org/10.1007/BF00130359
Publication status	Published - 1995 Jul
Externally published	Yes

ASJC Scopus subject areas

Biotechnology
Bioengineering
Applied Microbiology and Biotechnology

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title = "Manipulation of the genes for poly-β-hydroxybutyric acid synthesis in Alcaligenes eutrophus",

abstract = "In order to establish the molecular breeding system in Alcaligenes eutrophus producing poly-β-hydroxybutyric acid (PHB), phbCAB genes from A. eutrophus were recombined into the E. coli-A. eutrophus shuttle vector and directly transferred into A. eutrophus by the electroporation. In A. eutrophus transformants, recombinant plasmids were stably maintained and enzyme activities for PHB biosyntheses were elevated 1.4-2.7 fold by the cloned genes.",

author = "Park, {Hae Chul} and Park, {Jin Seo} and Lee, {Yong Hyun} and Huh, {Tae Lin}",

year = "1995",

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language = "English",

volume = "17",

pages = "729--734",

journal = "Biotechnology Letters",

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T1 - Manipulation of the genes for poly-β-hydroxybutyric acid synthesis in Alcaligenes eutrophus

AU - Park, Hae Chul

AU - Park, Jin Seo

AU - Lee, Yong Hyun

AU - Huh, Tae Lin

PY - 1995/7

Y1 - 1995/7

N2 - In order to establish the molecular breeding system in Alcaligenes eutrophus producing poly-β-hydroxybutyric acid (PHB), phbCAB genes from A. eutrophus were recombined into the E. coli-A. eutrophus shuttle vector and directly transferred into A. eutrophus by the electroporation. In A. eutrophus transformants, recombinant plasmids were stably maintained and enzyme activities for PHB biosyntheses were elevated 1.4-2.7 fold by the cloned genes.

AB - In order to establish the molecular breeding system in Alcaligenes eutrophus producing poly-β-hydroxybutyric acid (PHB), phbCAB genes from A. eutrophus were recombined into the E. coli-A. eutrophus shuttle vector and directly transferred into A. eutrophus by the electroporation. In A. eutrophus transformants, recombinant plasmids were stably maintained and enzyme activities for PHB biosyntheses were elevated 1.4-2.7 fold by the cloned genes.

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U2 - 10.1007/BF00130359

DO - 10.1007/BF00130359

M3 - Article

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JO - Biotechnology Letters

JF - Biotechnology Letters

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ER -

Manipulation of the genes for poly-β-hydroxybutyric acid synthesis in Alcaligenes eutrophus

Abstract

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