TY - JOUR
T1 - Marinobacter halodurans Sp. Nov., a halophilic bacterium isolated from sediment of a salt flat
AU - Yoo, Yeonjae
AU - Lee, Hanbyul
AU - Kwon, Bong Oh
AU - Khim, Jong Seong
AU - Baek, Seungwoo
AU - Pathiraja, Duleepa
AU - Park, Byeonghyeok
AU - Choi, In Geol
AU - Kim, Gyu Hyeok
AU - Kim, Beom Seok
AU - Kim, Jae Jin
N1 - Funding Information:
This research was funded by the Ministry of Oceans and Fisheries of Korea (MOF) [20170318 and 20170325], and a Korea University Grant.
Publisher Copyright:
© 2020 The Authors.
PY - 2020
Y1 - 2020
N2 - A Gram-staining-negative, aerobic, cream-coloured, marine bacterium, with rod-shaped cells, designated strain YJ-S3-2T, was isolated from salt flat sediment of Yongyu-do, Republic of Korea. YJ-S3-2T grew at pH 5.0–9.0 (optimum pH 7.0), 4–45 °C (optimum 30 °C) and with 1–18% (w/v) NaCl (optimum 6%). The results of 16S rRNA gene sequence analysis indicated that YJ-S3-2T was closely related to Marinobacter segnicrescens SS011B1-4T (97.0%) followed by, 'Marinobacter nanhaiticus' D15-8W (96.7%), Marinobacter bryozoorum 50-11T (96.7%), Marinobacter koreensis DSMZ 179240T T (96.5%) and Marinobacter bohaiensis T17T (96.5%). The average nucleotide identity (ANI) and the genome to genome distance calculator (GGDC) estimate values between YJ-S3-2T and related type strains were 73.7–79.8 and 19.9–22.5%, and also 73.5 and 20.7% with Marinobacter hydro-carbonoclasticus. YJ-S3-2T was characterized as having Q-9 as the predominant respiratory quinone and the principal fatty acids (>10%) were C16:0 (22.3%), summed feature 9 (C17:1 iso ω9c/C16:0 10-methyl, 13.8%) and 3 (C16:1 ω7c/C16:1 ω6c, 11.9%). The polar lipids consisted of diphosphatidylglycerol, phosphatidylethanolamine, phosphatidylglycerol, two unidentified aminolipids and two unidentified phospholipids. The DNA G+C content of YJ-S3-2T is 60.9 mol%. On the basis of the polyphasic taxonomic evidence presented in this study, YJ-S3-2T should be classified as representing a novel species within the genus Marinobacter, for which name Marinobacter halodurans sp. nov. is proposed, with the type strain YJ-S3-2T (=KACC 19883T=KCTC 62937T=JCM 33109T).
AB - A Gram-staining-negative, aerobic, cream-coloured, marine bacterium, with rod-shaped cells, designated strain YJ-S3-2T, was isolated from salt flat sediment of Yongyu-do, Republic of Korea. YJ-S3-2T grew at pH 5.0–9.0 (optimum pH 7.0), 4–45 °C (optimum 30 °C) and with 1–18% (w/v) NaCl (optimum 6%). The results of 16S rRNA gene sequence analysis indicated that YJ-S3-2T was closely related to Marinobacter segnicrescens SS011B1-4T (97.0%) followed by, 'Marinobacter nanhaiticus' D15-8W (96.7%), Marinobacter bryozoorum 50-11T (96.7%), Marinobacter koreensis DSMZ 179240T T (96.5%) and Marinobacter bohaiensis T17T (96.5%). The average nucleotide identity (ANI) and the genome to genome distance calculator (GGDC) estimate values between YJ-S3-2T and related type strains were 73.7–79.8 and 19.9–22.5%, and also 73.5 and 20.7% with Marinobacter hydro-carbonoclasticus. YJ-S3-2T was characterized as having Q-9 as the predominant respiratory quinone and the principal fatty acids (>10%) were C16:0 (22.3%), summed feature 9 (C17:1 iso ω9c/C16:0 10-methyl, 13.8%) and 3 (C16:1 ω7c/C16:1 ω6c, 11.9%). The polar lipids consisted of diphosphatidylglycerol, phosphatidylethanolamine, phosphatidylglycerol, two unidentified aminolipids and two unidentified phospholipids. The DNA G+C content of YJ-S3-2T is 60.9 mol%. On the basis of the polyphasic taxonomic evidence presented in this study, YJ-S3-2T should be classified as representing a novel species within the genus Marinobacter, for which name Marinobacter halodurans sp. nov. is proposed, with the type strain YJ-S3-2T (=KACC 19883T=KCTC 62937T=JCM 33109T).
KW - Halophilic bacteria
KW - Marinobacter
KW - Proteobacteira
KW - Salt flat
UR - http://www.scopus.com/inward/record.url?scp=85098246765&partnerID=8YFLogxK
U2 - 10.1099/ijsem.0.004530
DO - 10.1099/ijsem.0.004530
M3 - Article
C2 - 33079031
AN - SCOPUS:85098246765
SN - 1466-5026
VL - 70
SP - 6294
EP - 6300
JO - International Journal of Systematic and Evolutionary Microbiology
JF - International Journal of Systematic and Evolutionary Microbiology
IS - 12
M1 - 004530
ER -
