Marinobacter halodurans Sp. Nov., a halophilic bacterium isolated from sediment of a salt flat

Yeonjae Yoo; Hanbyul Lee; Bong Oh Kwon; Jong Seong Khim; Seungwoo Baek; Duleepa Pathiraja; Byeonghyeok Park; In Geol Choi; Gyu Hyeok Kim; Beom Seok Kim; Jae Jin Kim

doi:10.1099/ijsem.0.004530

Marinobacter halodurans Sp. Nov., a halophilic bacterium isolated from sediment of a salt flat

Yeonjae Yoo, Hanbyul Lee, Bong Oh Kwon, Jong Seong Khim, Seungwoo Baek, Duleepa Pathiraja, Byeonghyeok Park, In Geol Choi, Gyu Hyeok Kim, Beom Seok Kim, Jae Jin Kim

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Abstract

A Gram-staining-negative, aerobic, cream-coloured, marine bacterium, with rod-shaped cells, designated strain YJ-S3-2^T, was isolated from salt flat sediment of Yongyu-do, Republic of Korea. YJ-S3-2^T grew at pH 5.0–9.0 (optimum pH 7.0), 4–45 °C (optimum 30 °C) and with 1–18% (w/v) NaCl (optimum 6%). The results of 16S rRNA gene sequence analysis indicated that YJ-S3-2^T was closely related to Marinobacter segnicrescens SS011B1-4^T (97.0%) followed by, 'Marinobacter nanhaiticus' D15-8W (96.7%), Marinobacter bryozoorum 50-11^T (96.7%), Marinobacter koreensis DSMZ 179240^{T T} (96.5%) and Marinobacter bohaiensis T17^T (96.5%). The average nucleotide identity (ANI) and the genome to genome distance calculator (GGDC) estimate values between YJ-S3-2^T and related type strains were 73.7–79.8 and 19.9–22.5%, and also 73.5 and 20.7% with Marinobacter hydro-carbonoclasticus. YJ-S3-2^T was characterized as having Q-9 as the predominant respiratory quinone and the principal fatty acids (>10%) were C_16:0 (22.3%), summed feature 9 (C_17:1 iso ω9c/C_16:0 10-methyl, 13.8%) and 3 (C_16:1 ω7c/C1_6:1 ω6c, 11.9%). The polar lipids consisted of diphosphatidylglycerol, phosphatidylethanolamine, phosphatidylglycerol, two unidentified aminolipids and two unidentified phospholipids. The DNA G+C content of YJ-S3-2^T is 60.9 mol%. On the basis of the polyphasic taxonomic evidence presented in this study, YJ-S3-2^T should be classified as representing a novel species within the genus Marinobacter, for which name Marinobacter halodurans sp. nov. is proposed, with the type strain YJ-S3-2^T (=KACC 19883^T=KCTC 62937^T=JCM 33109^T).

Original language	English
Article number	004530
Pages (from-to)	6294-6300
Number of pages	7
Journal	International Journal of Systematic and Evolutionary Microbiology
Volume	70
Issue number	12
DOIs	https://doi.org/10.1099/ijsem.0.004530
Publication status	Published - 2020

Bibliographical note

Funding Information:
This research was funded by the Ministry of Oceans and Fisheries of Korea (MOF) [20170318 and 20170325], and a Korea University Grant.

Publisher Copyright:
© 2020 The Authors.

Keywords

Halophilic bacteria
Marinobacter
Proteobacteira
Salt flat

ASJC Scopus subject areas

Microbiology
Ecology, Evolution, Behavior and Systematics

UN SDGs

This output contributes to the following UN Sustainable Development Goals (SDGs)

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10.1099/ijsem.0.004530

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Yoo, Y., Lee, H., Kwon, B. O., Khim, J. S., Baek, S., Pathiraja, D., Park, B., Choi, I. G., Kim, G. H., Kim, B. S., & Kim, J. J. (2020). Marinobacter halodurans Sp. Nov., a halophilic bacterium isolated from sediment of a salt flat. International Journal of Systematic and Evolutionary Microbiology, 70(12), 6294-6300. Article 004530. https://doi.org/10.1099/ijsem.0.004530

@article{0db2cf3420a844b5ade89c54eae33a1b,

title = "Marinobacter halodurans Sp. Nov., a halophilic bacterium isolated from sediment of a salt flat",

abstract = "A Gram-staining-negative, aerobic, cream-coloured, marine bacterium, with rod-shaped cells, designated strain YJ-S3-2T, was isolated from salt flat sediment of Yongyu-do, Republic of Korea. YJ-S3-2T grew at pH 5.0–9.0 (optimum pH 7.0), 4–45 °C (optimum 30 °C) and with 1–18% (w/v) NaCl (optimum 6%). The results of 16S rRNA gene sequence analysis indicated that YJ-S3-2T was closely related to Marinobacter segnicrescens SS011B1-4T (97.0%) followed by, 'Marinobacter nanhaiticus' D15-8W (96.7%), Marinobacter bryozoorum 50-11T (96.7%), Marinobacter koreensis DSMZ 179240T T (96.5%) and Marinobacter bohaiensis T17T (96.5%). The average nucleotide identity (ANI) and the genome to genome distance calculator (GGDC) estimate values between YJ-S3-2T and related type strains were 73.7–79.8 and 19.9–22.5%, and also 73.5 and 20.7% with Marinobacter hydro-carbonoclasticus. YJ-S3-2T was characterized as having Q-9 as the predominant respiratory quinone and the principal fatty acids (>10%) were C16:0 (22.3%), summed feature 9 (C17:1 iso ω9c/C16:0 10-methyl, 13.8%) and 3 (C16:1 ω7c/C16:1 ω6c, 11.9%). The polar lipids consisted of diphosphatidylglycerol, phosphatidylethanolamine, phosphatidylglycerol, two unidentified aminolipids and two unidentified phospholipids. The DNA G+C content of YJ-S3-2T is 60.9 mol%. On the basis of the polyphasic taxonomic evidence presented in this study, YJ-S3-2T should be classified as representing a novel species within the genus Marinobacter, for which name Marinobacter halodurans sp. nov. is proposed, with the type strain YJ-S3-2T (=KACC 19883T=KCTC 62937T=JCM 33109T).",

keywords = "Halophilic bacteria, Marinobacter, Proteobacteira, Salt flat",

author = "Yeonjae Yoo and Hanbyul Lee and Kwon, {Bong Oh} and Khim, {Jong Seong} and Seungwoo Baek and Duleepa Pathiraja and Byeonghyeok Park and Choi, {In Geol} and Kim, {Gyu Hyeok} and Kim, {Beom Seok} and Kim, {Jae Jin}",

note = "Funding Information: This research was funded by the Ministry of Oceans and Fisheries of Korea (MOF) [20170318 and 20170325], and a Korea University Grant. Publisher Copyright: {\textcopyright} 2020 The Authors.",

year = "2020",

doi = "10.1099/ijsem.0.004530",

language = "English",

volume = "70",

pages = "6294--6300",

journal = "International Journal of Systematic and Evolutionary Microbiology",

issn = "1466-5026",

publisher = "Microbiology Society",

number = "12",

}

TY - JOUR

T1 - Marinobacter halodurans Sp. Nov., a halophilic bacterium isolated from sediment of a salt flat

AU - Yoo, Yeonjae

AU - Lee, Hanbyul

AU - Kwon, Bong Oh

AU - Khim, Jong Seong

AU - Baek, Seungwoo

AU - Pathiraja, Duleepa

AU - Park, Byeonghyeok

AU - Choi, In Geol

AU - Kim, Gyu Hyeok

AU - Kim, Beom Seok

AU - Kim, Jae Jin

N1 - Funding Information: This research was funded by the Ministry of Oceans and Fisheries of Korea (MOF) [20170318 and 20170325], and a Korea University Grant. Publisher Copyright: © 2020 The Authors.

PY - 2020

Y1 - 2020

N2 - A Gram-staining-negative, aerobic, cream-coloured, marine bacterium, with rod-shaped cells, designated strain YJ-S3-2T, was isolated from salt flat sediment of Yongyu-do, Republic of Korea. YJ-S3-2T grew at pH 5.0–9.0 (optimum pH 7.0), 4–45 °C (optimum 30 °C) and with 1–18% (w/v) NaCl (optimum 6%). The results of 16S rRNA gene sequence analysis indicated that YJ-S3-2T was closely related to Marinobacter segnicrescens SS011B1-4T (97.0%) followed by, 'Marinobacter nanhaiticus' D15-8W (96.7%), Marinobacter bryozoorum 50-11T (96.7%), Marinobacter koreensis DSMZ 179240T T (96.5%) and Marinobacter bohaiensis T17T (96.5%). The average nucleotide identity (ANI) and the genome to genome distance calculator (GGDC) estimate values between YJ-S3-2T and related type strains were 73.7–79.8 and 19.9–22.5%, and also 73.5 and 20.7% with Marinobacter hydro-carbonoclasticus. YJ-S3-2T was characterized as having Q-9 as the predominant respiratory quinone and the principal fatty acids (>10%) were C16:0 (22.3%), summed feature 9 (C17:1 iso ω9c/C16:0 10-methyl, 13.8%) and 3 (C16:1 ω7c/C16:1 ω6c, 11.9%). The polar lipids consisted of diphosphatidylglycerol, phosphatidylethanolamine, phosphatidylglycerol, two unidentified aminolipids and two unidentified phospholipids. The DNA G+C content of YJ-S3-2T is 60.9 mol%. On the basis of the polyphasic taxonomic evidence presented in this study, YJ-S3-2T should be classified as representing a novel species within the genus Marinobacter, for which name Marinobacter halodurans sp. nov. is proposed, with the type strain YJ-S3-2T (=KACC 19883T=KCTC 62937T=JCM 33109T).

AB - A Gram-staining-negative, aerobic, cream-coloured, marine bacterium, with rod-shaped cells, designated strain YJ-S3-2T, was isolated from salt flat sediment of Yongyu-do, Republic of Korea. YJ-S3-2T grew at pH 5.0–9.0 (optimum pH 7.0), 4–45 °C (optimum 30 °C) and with 1–18% (w/v) NaCl (optimum 6%). The results of 16S rRNA gene sequence analysis indicated that YJ-S3-2T was closely related to Marinobacter segnicrescens SS011B1-4T (97.0%) followed by, 'Marinobacter nanhaiticus' D15-8W (96.7%), Marinobacter bryozoorum 50-11T (96.7%), Marinobacter koreensis DSMZ 179240T T (96.5%) and Marinobacter bohaiensis T17T (96.5%). The average nucleotide identity (ANI) and the genome to genome distance calculator (GGDC) estimate values between YJ-S3-2T and related type strains were 73.7–79.8 and 19.9–22.5%, and also 73.5 and 20.7% with Marinobacter hydro-carbonoclasticus. YJ-S3-2T was characterized as having Q-9 as the predominant respiratory quinone and the principal fatty acids (>10%) were C16:0 (22.3%), summed feature 9 (C17:1 iso ω9c/C16:0 10-methyl, 13.8%) and 3 (C16:1 ω7c/C16:1 ω6c, 11.9%). The polar lipids consisted of diphosphatidylglycerol, phosphatidylethanolamine, phosphatidylglycerol, two unidentified aminolipids and two unidentified phospholipids. The DNA G+C content of YJ-S3-2T is 60.9 mol%. On the basis of the polyphasic taxonomic evidence presented in this study, YJ-S3-2T should be classified as representing a novel species within the genus Marinobacter, for which name Marinobacter halodurans sp. nov. is proposed, with the type strain YJ-S3-2T (=KACC 19883T=KCTC 62937T=JCM 33109T).

KW - Halophilic bacteria

KW - Marinobacter

KW - Proteobacteira

KW - Salt flat

UR - http://www.scopus.com/inward/record.url?scp=85098246765&partnerID=8YFLogxK

U2 - 10.1099/ijsem.0.004530

DO - 10.1099/ijsem.0.004530

M3 - Article

C2 - 33079031

AN - SCOPUS:85098246765

SN - 1466-5026

VL - 70

SP - 6294

EP - 6300

JO - International Journal of Systematic and Evolutionary Microbiology

JF - International Journal of Systematic and Evolutionary Microbiology

IS - 12

M1 - 004530

ER -

Marinobacter halodurans Sp. Nov., a halophilic bacterium isolated from sediment of a salt flat

Abstract

Bibliographical note

Keywords

ASJC Scopus subject areas

UN SDGs

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