TY - JOUR
T1 - Matrix-assisted laser desorption/ionization-mass spectrometry of cuticular lipid profiles can differentiate sex, age, and mating status of Anopheles gambiae mosquitoes
AU - Suarez, Estrella
AU - Nguyen, Hien P.
AU - Ortiz, Israel P.
AU - Lee, Kyu Jong
AU - Kim, Seoung Bum
AU - Krzywinski, Jaroslaw
AU - Schug, Kevin A.
PY - 2011/11/7
Y1 - 2011/11/7
N2 - Malaria is a devastating mosquito-borne disease, which affects hundreds of millions of people each year. It is transmitted predominantly by Anopheles gambiae, whose females must be >10 days old to become infective. In this study, cuticular lipids from a laboratory strain of this mosquito species were analyzed using a mass spectrometry method to evaluate their utility for age, sex and mating status differentiation. Matrix-assisted laser desorption/ionization-mass spectrometry (MALDI-MS), in conjunction with an acenaphthene/silver nitrate matrix preparation, was shown to be 100% effective in classifying A. gambiae females into 1, 7-10, and 14 days of age. MALDI-MS analysis, supported by multivariate statistical methods, was also effective in detecting cuticular lipid differences between the sexes and between virgin and mated females. The technique requires further testing, but the obtained results suggest that MALDI-MS cuticular lipid spectra could be used for age grading of A. gambiae females with precision greater than with other available methods.
AB - Malaria is a devastating mosquito-borne disease, which affects hundreds of millions of people each year. It is transmitted predominantly by Anopheles gambiae, whose females must be >10 days old to become infective. In this study, cuticular lipids from a laboratory strain of this mosquito species were analyzed using a mass spectrometry method to evaluate their utility for age, sex and mating status differentiation. Matrix-assisted laser desorption/ionization-mass spectrometry (MALDI-MS), in conjunction with an acenaphthene/silver nitrate matrix preparation, was shown to be 100% effective in classifying A. gambiae females into 1, 7-10, and 14 days of age. MALDI-MS analysis, supported by multivariate statistical methods, was also effective in detecting cuticular lipid differences between the sexes and between virgin and mated females. The technique requires further testing, but the obtained results suggest that MALDI-MS cuticular lipid spectra could be used for age grading of A. gambiae females with precision greater than with other available methods.
KW - Anopheles gambiae
KW - Cuticular hydrocarbons
KW - Cuticular lipids
KW - Malaria
KW - Matrix-assisted laser desorption/ionization
KW - Mosquito
KW - Principal component analysis
KW - Support vector machines
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U2 - 10.1016/j.aca.2011.08.033
DO - 10.1016/j.aca.2011.08.033
M3 - Article
C2 - 21995923
AN - SCOPUS:80053651675
SN - 0003-2670
VL - 706
SP - 157
EP - 163
JO - Analytica Chimica Acta
JF - Analytica Chimica Acta
IS - 1
ER -