Mechanical alteration of knee chondrocyte on hormone deficiency of growing rat using atomic force microscopy

Chi Hoon Kim, Dae Gon Woo, Ji Hyung Park, Ok Hee Jeon, Seung Hak Lee, Chi Hyun Kim, Dae Sung Yoon, Han Sung Kim

Research output: Contribution to journalArticlepeer-review


The present study was designed to evaluate the correlation between osteoporosis and osteoarthritis, based on micro-computed tomography (Micro-CT) and atomic force microscopy (AFM). To induce osteoporosis, virgin Sprague-Dawley rats (6 weeks old) were ovariectomized (OVX). All rats were randomly divided in two groups (normal group: 9, OVX group: 6). Knee joint on each rat was scanned by Micro-CT with 18-μm resolution at 0, 4 and 8 weeks. Morphological characteristics were calculated by Micro-CT image analyzer. Knee joint chondrocytes were isolated and immobilized to investigate morphological and mechanical characteristics on AFM measurements. In the OVX group, morphological characteristics significantly changed at all the femur over time(p<0.05). Comparing OVX group to normal group at 8 weeks, morphological characteristics and stiffness of chondrocytes measured by AFM significantly increased in normal group more than OVX group(p<0.05). The results showed that osteoporosis can cause osteoarthritis. The finding of the present study indicated that AFM can analyze the origin of osteoarthritis. In addition, this study is valuable attempt to find the correlation between osteoporosis and osteoarthritis.

Original languageEnglish
Pages (from-to)381-387
Number of pages7
JournalTissue Engineering and Regenerative Medicine
Issue number4
Publication statusPublished - 2010 Aug
Externally publishedYes


  • Atomic force microscopy (AFM)
  • Chondrocyte
  • Micro-CT
  • Osteoarthritis
  • Osteoporosis

ASJC Scopus subject areas

  • Medicine (miscellaneous)
  • Biomedical Engineering


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