Mechanosensitive physiology of chlamydomonas reinhardtii under direct membrane distortion

Seul Ki Min, Gwang Heum Yoon, Jung Hyun Joo, Sang Jun Sim, Hwa Sung Shin

Research output: Contribution to journalArticlepeer-review

23 Citations (Scopus)


Cellular membrane distortion invokes variations in cellular physiology. However, lack of an appropriate system to control the stress and facilitate molecular analyses has hampered progress of relevant studies. In this study, a microfluidic system that finely manipulates membrane distortion of Chlamydomonas reinhardtii (C. reinhardtii) was developed. The device facilitated a first-time demonstration that directs membrane distortion invokes variations in deflagellation, cell cycle, and lipid metabolism. C. reinhardtii showed a prolonged G 1 phase with an extended total cell cycle time, and upregulated Mat3 regulated a cell size and cell cycle. Additionally, increased TAG compensated for the loss of cell mass. Overall, this study suggest that cell biology that requires direct membrane distortion can be realized using this system, and the implication of cell cycle with Mat3 expression of C. reinhardtii was first demonstrated. Finally, membrane distortion can be an attractive inducer for biodiesel production since it is reliable and robust.

Original languageEnglish
Article number4675
JournalScientific reports
Publication statusPublished - 2014 Apr 14

Bibliographical note

Funding Information:
This research was supported by grants from the Korea CCS R&D Center (KCRC) grant funded by the Korean government (Ministry of Education, Science and Technology) (2012-0008937) and by the Marine Biotechnology Program funded by the Ministry of Land, Transport and Maritime Affairs, Korea.

ASJC Scopus subject areas

  • General


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