Mechanosensitive physiology of chlamydomonas reinhardtii under direct membrane distortion

Seul Ki Min; Gwang Heum Yoon; Jung Hyun Joo; Sang Jun Sim; Hwa Sung Shin

doi:10.1038/srep04675

Mechanosensitive physiology of chlamydomonas reinhardtii under direct membrane distortion

Seul Ki Min, Gwang Heum Yoon, Jung Hyun Joo, Sang Jun Sim, Hwa Sung Shin

Research output: Contribution to journal › Article › peer-review

25 Citations (Scopus)

Abstract

Cellular membrane distortion invokes variations in cellular physiology. However, lack of an appropriate system to control the stress and facilitate molecular analyses has hampered progress of relevant studies. In this study, a microfluidic system that finely manipulates membrane distortion of Chlamydomonas reinhardtii (C. reinhardtii) was developed. The device facilitated a first-time demonstration that directs membrane distortion invokes variations in deflagellation, cell cycle, and lipid metabolism. C. reinhardtii showed a prolonged G 1 phase with an extended total cell cycle time, and upregulated Mat3 regulated a cell size and cell cycle. Additionally, increased TAG compensated for the loss of cell mass. Overall, this study suggest that cell biology that requires direct membrane distortion can be realized using this system, and the implication of cell cycle with Mat3 expression of C. reinhardtii was first demonstrated. Finally, membrane distortion can be an attractive inducer for biodiesel production since it is reliable and robust.

Original language	English
Article number	4675
Journal	Scientific reports
Volume	4
DOIs	https://doi.org/10.1038/srep04675
Publication status	Published - 2014 Apr 14

Bibliographical note

Funding Information:
This research was supported by grants from the Korea CCS R&D Center (KCRC) grant funded by the Korean government (Ministry of Education, Science and Technology) (2012-0008937) and by the Marine Biotechnology Program funded by the Ministry of Land, Transport and Maritime Affairs, Korea.

ASJC Scopus subject areas

General

Access to Document

10.1038/srep04675

Cite this

@article{32475e744ffd41b3813a782eab2832d4,

title = "Mechanosensitive physiology of chlamydomonas reinhardtii under direct membrane distortion",

abstract = "Cellular membrane distortion invokes variations in cellular physiology. However, lack of an appropriate system to control the stress and facilitate molecular analyses has hampered progress of relevant studies. In this study, a microfluidic system that finely manipulates membrane distortion of Chlamydomonas reinhardtii (C. reinhardtii) was developed. The device facilitated a first-time demonstration that directs membrane distortion invokes variations in deflagellation, cell cycle, and lipid metabolism. C. reinhardtii showed a prolonged G 1 phase with an extended total cell cycle time, and upregulated Mat3 regulated a cell size and cell cycle. Additionally, increased TAG compensated for the loss of cell mass. Overall, this study suggest that cell biology that requires direct membrane distortion can be realized using this system, and the implication of cell cycle with Mat3 expression of C. reinhardtii was first demonstrated. Finally, membrane distortion can be an attractive inducer for biodiesel production since it is reliable and robust.",

author = "Min, {Seul Ki} and Yoon, {Gwang Heum} and Joo, {Jung Hyun} and Sim, {Sang Jun} and Shin, {Hwa Sung}",

note = "Funding Information: This research was supported by grants from the Korea CCS R&D Center (KCRC) grant funded by the Korean government (Ministry of Education, Science and Technology) (2012-0008937) and by the Marine Biotechnology Program funded by the Ministry of Land, Transport and Maritime Affairs, Korea.",

year = "2014",

month = apr,

day = "14",

doi = "10.1038/srep04675",

language = "English",

volume = "4",

journal = "Scientific reports",

issn = "2045-2322",

publisher = "Nature Research",

}

TY - JOUR

T1 - Mechanosensitive physiology of chlamydomonas reinhardtii under direct membrane distortion

AU - Min, Seul Ki

AU - Yoon, Gwang Heum

AU - Joo, Jung Hyun

AU - Sim, Sang Jun

AU - Shin, Hwa Sung

N1 - Funding Information: This research was supported by grants from the Korea CCS R&D Center (KCRC) grant funded by the Korean government (Ministry of Education, Science and Technology) (2012-0008937) and by the Marine Biotechnology Program funded by the Ministry of Land, Transport and Maritime Affairs, Korea.

PY - 2014/4/14

Y1 - 2014/4/14

N2 - Cellular membrane distortion invokes variations in cellular physiology. However, lack of an appropriate system to control the stress and facilitate molecular analyses has hampered progress of relevant studies. In this study, a microfluidic system that finely manipulates membrane distortion of Chlamydomonas reinhardtii (C. reinhardtii) was developed. The device facilitated a first-time demonstration that directs membrane distortion invokes variations in deflagellation, cell cycle, and lipid metabolism. C. reinhardtii showed a prolonged G 1 phase with an extended total cell cycle time, and upregulated Mat3 regulated a cell size and cell cycle. Additionally, increased TAG compensated for the loss of cell mass. Overall, this study suggest that cell biology that requires direct membrane distortion can be realized using this system, and the implication of cell cycle with Mat3 expression of C. reinhardtii was first demonstrated. Finally, membrane distortion can be an attractive inducer for biodiesel production since it is reliable and robust.

AB - Cellular membrane distortion invokes variations in cellular physiology. However, lack of an appropriate system to control the stress and facilitate molecular analyses has hampered progress of relevant studies. In this study, a microfluidic system that finely manipulates membrane distortion of Chlamydomonas reinhardtii (C. reinhardtii) was developed. The device facilitated a first-time demonstration that directs membrane distortion invokes variations in deflagellation, cell cycle, and lipid metabolism. C. reinhardtii showed a prolonged G 1 phase with an extended total cell cycle time, and upregulated Mat3 regulated a cell size and cell cycle. Additionally, increased TAG compensated for the loss of cell mass. Overall, this study suggest that cell biology that requires direct membrane distortion can be realized using this system, and the implication of cell cycle with Mat3 expression of C. reinhardtii was first demonstrated. Finally, membrane distortion can be an attractive inducer for biodiesel production since it is reliable and robust.

UR - http://www.scopus.com/inward/record.url?scp=84898621278&partnerID=8YFLogxK

U2 - 10.1038/srep04675

DO - 10.1038/srep04675

M3 - Article

C2 - 24728350

AN - SCOPUS:84898621278

SN - 2045-2322

VL - 4

JO - Scientific reports

JF - Scientific reports

M1 - 4675

ER -

Mechanosensitive physiology of chlamydomonas reinhardtii under direct membrane distortion

Abstract

Bibliographical note

ASJC Scopus subject areas

Access to Document

Other files and links

Fingerprint

Cite this