TY - JOUR
T1 - Medium optimization for the production of probiotic lactobacillus acidophilus a12 using response surface methodology
AU - Lee, Na Kyoung
AU - Park, Yeo Lang
AU - Choe, Ga Jin
AU - Chang, Hyo Ihl
AU - Paik, Hyun Dong
N1 - Copyright:
Copyright 2017 Elsevier B.V., All rights reserved.
PY - 2010/6
Y1 - 2010/6
N2 - Lactobacillus acidophilus A12 was isolated from chicken feces for use as an immunostimulating livestock probiotic. The purpose of this study was to optimize the production of L. acidophilus A12 using response surface methodology (RSM). Initially, the influence of growth medium was studied in terms of carbon sources (glucose, fructose, lactose, glycerol, sucrose, ethanol, and mannitol), nitrogen sources (beef extract, yeast extract, malt extract, and tryptone), and inorganic salts (CaCl2, MgSO4, KH2PO 4, (NH4)2SO4, FeSO4, and NaCl). Through one factor-at-a time experiment, lactose, yeast extract, and CaCl2 were determined to be the best sources of carbon, nitrogen, and inorganic salt, respectively. The optimum composition was found to be 17.7 g/L lactose, 18.6 g/L yeast extract, and 0.9 g/L CaCl2. Under these conditions, a maximum cell density of 9.33 Log CFU/mL was produced, similar to the predicted value.
AB - Lactobacillus acidophilus A12 was isolated from chicken feces for use as an immunostimulating livestock probiotic. The purpose of this study was to optimize the production of L. acidophilus A12 using response surface methodology (RSM). Initially, the influence of growth medium was studied in terms of carbon sources (glucose, fructose, lactose, glycerol, sucrose, ethanol, and mannitol), nitrogen sources (beef extract, yeast extract, malt extract, and tryptone), and inorganic salts (CaCl2, MgSO4, KH2PO 4, (NH4)2SO4, FeSO4, and NaCl). Through one factor-at-a time experiment, lactose, yeast extract, and CaCl2 were determined to be the best sources of carbon, nitrogen, and inorganic salt, respectively. The optimum composition was found to be 17.7 g/L lactose, 18.6 g/L yeast extract, and 0.9 g/L CaCl2. Under these conditions, a maximum cell density of 9.33 Log CFU/mL was produced, similar to the predicted value.
KW - Chicken feces
KW - Lactobacillus acidophilus
KW - Medium optimization
KW - Probiotics
KW - Response surface methodology
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U2 - 10.5851/kosfa.2010.30.3.359
DO - 10.5851/kosfa.2010.30.3.359
M3 - Article
AN - SCOPUS:79960760183
SN - 2636-0772
VL - 30
SP - 359
EP - 364
JO - Food Science of Animal Resources
JF - Food Science of Animal Resources
IS - 3
ER -