Menthol, a naturally occurring monoterpene, is used in foods, cosmetic products, and topical therapeutic preparations. It also exerts cytotoxic activity against several cancer cell types, including prostate cancer cells. However, little is known about the mechanism of menthol action against prostate cancer cells. In this study, we investigated the effect of menthol on the gene expression profile of PC-3 prostate cancer cells using DNA microarray analyses. Gene set enrichment analysis revealed that menthol primarily affects the expression of cell cycle-related genes. Subsequent experimental analyses validated that menthol induces G2/M arrest. Particularly, menthol markedly down-regulated polo-like kinase 1 (PLK1), a key regulator of G2/M phase progression and inhibited its downstream signaling. Our computational analyses and experimental validation provide a basis for future investigations that are aimed at elucidating the action of menthol on cell cycle control in prostate cancer cells.
|Number of pages||6|
|Journal||Biochemical and biophysical research communications|
|Publication status||Published - 2012 Jun 8|
Bibliographical noteFunding Information:
This research was supported by the National Research Foundation of Korea (NRF) funded by the Korea government (MEST) ( 2009-0086652 , 2010-0019472 , and 2010-0021234 ). SRP was supported by a training fellowship from the United States National Institutes of Health ( 5T32CA093247 ).
- DNA microarray
- Gene set enrichment analysis
- Prostate cancer
ASJC Scopus subject areas
- Molecular Biology
- Cell Biology