Menthol induces cell-cycle arrest in PC-3 cells by down-regulating G2/M genes, including polo-like kinase 1

Su Hwa Kim; Sanghoon Lee; Stephen R. Piccolo; Kristina Allen-Brady; Eun Jung Park; Jung Nyeo Chun; Tae Woo Kim; Nam Hyuk Cho; In Gyu Kim; Insuk So; Ju Hong Jeon

doi:10.1016/j.bbrc.2012.05.010

Menthol induces cell-cycle arrest in PC-3 cells by down-regulating G2/M genes, including polo-like kinase 1

Su Hwa Kim, Sanghoon Lee, Stephen R. Piccolo, Kristina Allen-Brady, Eun Jung Park, Jung Nyeo Chun, Tae Woo Kim, Nam Hyuk Cho, In Gyu Kim, Insuk So, Ju Hong Jeon

Department of Biomedical Sciences

Research output: Contribution to journal › Article › peer-review

25 Citations (Scopus)

Abstract

Menthol, a naturally occurring monoterpene, is used in foods, cosmetic products, and topical therapeutic preparations. It also exerts cytotoxic activity against several cancer cell types, including prostate cancer cells. However, little is known about the mechanism of menthol action against prostate cancer cells. In this study, we investigated the effect of menthol on the gene expression profile of PC-3 prostate cancer cells using DNA microarray analyses. Gene set enrichment analysis revealed that menthol primarily affects the expression of cell cycle-related genes. Subsequent experimental analyses validated that menthol induces G2/M arrest. Particularly, menthol markedly down-regulated polo-like kinase 1 (PLK1), a key regulator of G2/M phase progression and inhibited its downstream signaling. Our computational analyses and experimental validation provide a basis for future investigations that are aimed at elucidating the action of menthol on cell cycle control in prostate cancer cells.

Original language	English
Pages (from-to)	436-441
Number of pages	6
Journal	Biochemical and biophysical research communications
Volume	422
Issue number	3
DOIs	https://doi.org/10.1016/j.bbrc.2012.05.010
Publication status	Published - 2012 Jun 8

Bibliographical note

Funding Information:
This research was supported by the National Research Foundation of Korea (NRF) funded by the Korea government (MEST) ( 2009-0086652 , 2010-0019472 , and 2010-0021234 ). SRP was supported by a training fellowship from the United States National Institutes of Health ( 5T32CA093247 ).

Keywords

DNA microarray
Gene set enrichment analysis
Menthol
PLK1
Prostate cancer

ASJC Scopus subject areas

Biophysics
Biochemistry
Molecular Biology
Cell Biology

UN SDGs

This output contributes to the following UN Sustainable Development Goals (SDGs)

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10.1016/j.bbrc.2012.05.010

Cite this

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title = "Menthol induces cell-cycle arrest in PC-3 cells by down-regulating G2/M genes, including polo-like kinase 1",

abstract = "Menthol, a naturally occurring monoterpene, is used in foods, cosmetic products, and topical therapeutic preparations. It also exerts cytotoxic activity against several cancer cell types, including prostate cancer cells. However, little is known about the mechanism of menthol action against prostate cancer cells. In this study, we investigated the effect of menthol on the gene expression profile of PC-3 prostate cancer cells using DNA microarray analyses. Gene set enrichment analysis revealed that menthol primarily affects the expression of cell cycle-related genes. Subsequent experimental analyses validated that menthol induces G2/M arrest. Particularly, menthol markedly down-regulated polo-like kinase 1 (PLK1), a key regulator of G2/M phase progression and inhibited its downstream signaling. Our computational analyses and experimental validation provide a basis for future investigations that are aimed at elucidating the action of menthol on cell cycle control in prostate cancer cells.",

keywords = "DNA microarray, Gene set enrichment analysis, Menthol, PLK1, Prostate cancer",

author = "Kim, {Su Hwa} and Sanghoon Lee and Piccolo, {Stephen R.} and Kristina Allen-Brady and Park, {Eun Jung} and Chun, {Jung Nyeo} and Kim, {Tae Woo} and Cho, {Nam Hyuk} and Kim, {In Gyu} and Insuk So and Jeon, {Ju Hong}",

note = "Funding Information: This research was supported by the National Research Foundation of Korea (NRF) funded by the Korea government (MEST) ( 2009-0086652 , 2010-0019472 , and 2010-0021234 ). SRP was supported by a training fellowship from the United States National Institutes of Health ( 5T32CA093247 ). ",

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doi = "10.1016/j.bbrc.2012.05.010",

language = "English",

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AU - Kim, Su Hwa

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AU - Allen-Brady, Kristina

AU - Park, Eun Jung

AU - Chun, Jung Nyeo

AU - Kim, Tae Woo

AU - Cho, Nam Hyuk

AU - Kim, In Gyu

AU - So, Insuk

AU - Jeon, Ju Hong

N1 - Funding Information: This research was supported by the National Research Foundation of Korea (NRF) funded by the Korea government (MEST) ( 2009-0086652 , 2010-0019472 , and 2010-0021234 ). SRP was supported by a training fellowship from the United States National Institutes of Health ( 5T32CA093247 ).

PY - 2012/6/8

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N2 - Menthol, a naturally occurring monoterpene, is used in foods, cosmetic products, and topical therapeutic preparations. It also exerts cytotoxic activity against several cancer cell types, including prostate cancer cells. However, little is known about the mechanism of menthol action against prostate cancer cells. In this study, we investigated the effect of menthol on the gene expression profile of PC-3 prostate cancer cells using DNA microarray analyses. Gene set enrichment analysis revealed that menthol primarily affects the expression of cell cycle-related genes. Subsequent experimental analyses validated that menthol induces G2/M arrest. Particularly, menthol markedly down-regulated polo-like kinase 1 (PLK1), a key regulator of G2/M phase progression and inhibited its downstream signaling. Our computational analyses and experimental validation provide a basis for future investigations that are aimed at elucidating the action of menthol on cell cycle control in prostate cancer cells.

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Menthol induces cell-cycle arrest in PC-3 cells by down-regulating G2/M genes, including polo-like kinase 1

Abstract

Bibliographical note

Keywords

ASJC Scopus subject areas

UN SDGs

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