Metabolic engineering of Enterobacter aerogenes to improve the production of 2,3-butanediol

Laxmi Prasad Thapa; Sang Jun Lee; Chulhwan Park; Seung Wook Kim

doi:10.1016/j.bej.2018.12.019

Metabolic engineering of Enterobacter aerogenes to improve the production of 2,3-butanediol

Laxmi Prasad Thapa, Sang Jun Lee, Chulhwan Park, Seung Wook Kim

Research output: Contribution to journal › Article › peer-review

21 Citations (Scopus)

Abstract

The enhanced production of 2,3-butanediol was investigated using a metabolic engineering approach and optimized fermentation conditions. New engineered strains of Enterobacter aerogenes ATCC 29007 were developed by deleting the D-lactate dehydrogenase (ldhA), phosphate acetyltransferase (pta), malate dehydrogenase (mdh), and acetaldehyde dehydrogenase (acdh) genes to block the production of lactate, acetate, succinate, and ethanol, respectively. The resulting engineered strain E. aerogenes SUMI02 (ΔldhAΔpta) produced 36.5 g/L of 2,3-butanediol in flask cultivation, an amount 8.11 times greater than that of its wild type counterpart (4.5 g/L). In addition, the 2,3-butanediol production and productivity reached 38.24 g/L and 0.8 g/L/h, respectively, in the batch fermentation using a bioreactor.

Original language	English
Pages (from-to)	169-178
Number of pages	10
Journal	Biochemical Engineering Journal
Volume	143
DOIs	https://doi.org/10.1016/j.bej.2018.12.019
Publication status	Published - 2019 Mar 15

Bibliographical note

Funding Information:
This research was supported by Basic Science Research Program through the National Research Foundation of Korea (NRF) funded by the Ministry of Science and ICT ( 2014R1A2A2A01007321 ) and the Ministry of Education ( 2016R1D1A1A09918327 ).

Funding Information:
This research was supported by Basic Science Research Program through the National Research Foundation of Korea (NRF) funded by the Ministry of Science and ICT (2014R1A2A2A01007321) and the Ministry of Education (2016R1D1A1A09918327).

Publisher Copyright:
© 2018 Elsevier B.V.

Keywords

2,3-Butanediol
Biorefinery
Enterobacter aerogenes
Metabolic engineering

ASJC Scopus subject areas

Biotechnology
Environmental Engineering
Bioengineering
Biomedical Engineering

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10.1016/j.bej.2018.12.019

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title = "Metabolic engineering of Enterobacter aerogenes to improve the production of 2,3-butanediol",

abstract = "The enhanced production of 2,3-butanediol was investigated using a metabolic engineering approach and optimized fermentation conditions. New engineered strains of Enterobacter aerogenes ATCC 29007 were developed by deleting the D-lactate dehydrogenase (ldhA), phosphate acetyltransferase (pta), malate dehydrogenase (mdh), and acetaldehyde dehydrogenase (acdh) genes to block the production of lactate, acetate, succinate, and ethanol, respectively. The resulting engineered strain E. aerogenes SUMI02 (ΔldhAΔpta) produced 36.5 g/L of 2,3-butanediol in flask cultivation, an amount 8.11 times greater than that of its wild type counterpart (4.5 g/L). In addition, the 2,3-butanediol production and productivity reached 38.24 g/L and 0.8 g/L/h, respectively, in the batch fermentation using a bioreactor.",

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N1 - Funding Information: This research was supported by Basic Science Research Program through the National Research Foundation of Korea (NRF) funded by the Ministry of Science and ICT ( 2014R1A2A2A01007321 ) and the Ministry of Education ( 2016R1D1A1A09918327 ). Funding Information: This research was supported by Basic Science Research Program through the National Research Foundation of Korea (NRF) funded by the Ministry of Science and ICT (2014R1A2A2A01007321) and the Ministry of Education (2016R1D1A1A09918327). Publisher Copyright: © 2018 Elsevier B.V.

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N2 - The enhanced production of 2,3-butanediol was investigated using a metabolic engineering approach and optimized fermentation conditions. New engineered strains of Enterobacter aerogenes ATCC 29007 were developed by deleting the D-lactate dehydrogenase (ldhA), phosphate acetyltransferase (pta), malate dehydrogenase (mdh), and acetaldehyde dehydrogenase (acdh) genes to block the production of lactate, acetate, succinate, and ethanol, respectively. The resulting engineered strain E. aerogenes SUMI02 (ΔldhAΔpta) produced 36.5 g/L of 2,3-butanediol in flask cultivation, an amount 8.11 times greater than that of its wild type counterpart (4.5 g/L). In addition, the 2,3-butanediol production and productivity reached 38.24 g/L and 0.8 g/L/h, respectively, in the batch fermentation using a bioreactor.

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Metabolic engineering of Enterobacter aerogenes to improve the production of 2,3-butanediol

Abstract

Bibliographical note

Keywords

ASJC Scopus subject areas

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