Metabolic engineering of Klebsiella pneumoniae for the production of cis,cis-muconic acid

Hwi Min Jung, Moo Young Jung, Min Kyu Oh

Research output: Contribution to journalArticlepeer-review

29 Citations (Scopus)


cis,cis-Muconic acid (ccMA), a metabolic intermediate of Klebsiella pneumoniae, can be converted to adipic acid and terephthalic acid, which are important monomers of synthetic polymers. However, wild-type K. pneumoniae does not produce ccMA because intracellular carbon flow does not favor ccMA biosynthesis. In this study, several metabolic engineering strategies were used in an attempt to modify the wild-type strain to induce it to produce ccMA. First, by blocking the synthesis of aromatic amino acids, 343 mg/L of catechol, a precursor of ccMA, was produced. Then, the native catechol 1,2-dioxygenasegene (catA) was overexpressed, which caused the strain to convert the catechol to ccMA. The production of ccMA was further improved by deletion of the muconate cycloisomerase gene (catB) and by deleting a feedback inhibitor of the aromatic amino acid pathway. Further improvement was achieved by adjusting the pH of the culture broth. The developed strain produced 2.1 g/L of ccMA in flask cultivation. The results showed the potential of K. pneumoniae as a ccMA producer.

Original languageEnglish
Pages (from-to)5217-5225
Number of pages9
JournalApplied Microbiology and Biotechnology
Issue number12
Publication statusPublished - 2015 Jun 28

Bibliographical note

Funding Information:
This work was supported by grants from the National Research Foundation of Korea funded by the Korean Government (2012M1A2A2026560 and 2014R1A2A2A03007094).

Publisher Copyright:
© 2015, Springer-Verlag Berlin Heidelberg.


  • Aromatic amino acid pathway
  • Benzoate degradation pathway
  • Klebsiella pneumoniae
  • cis,cis-Muconic acid

ASJC Scopus subject areas

  • Biotechnology
  • Applied Microbiology and Biotechnology


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