TY - JOUR
T1 - Metabolic engineering of Streptomyces venezuelae for malonyl-CoA biosynthesis to enhance heterologous production of polyketides
AU - Maharjan, Sushila
AU - Park, Je Won
AU - Yoon, Yeo Joon
AU - Lee, Hei Chan
AU - Sohng, Jae Kyung
N1 - Funding Information:
Acknowledgments This work was supported by Agenda program (No. 200905FP014), Rural Development Administration, Republic of Korea and Korean Research Foundation (KRF) by providing research scholarship for a PhD scholar.
Copyright:
Copyright 2010 Elsevier B.V., All rights reserved.
PY - 2010/1
Y1 - 2010/1
N2 - Using metabolic engineering, we developed Streptomyces venezuelae YJ028 as an efficient heterologous host to increase the malonyl-CoA pool to be directed towards enhanced production of various polyketides. To probe the applicability of newly developed hosts in the heterologous production of polyketides, we expressed type III polyketide synthase, 1,3,6,8-tetrahydroxynaphthalene synthase, in these hosts. Flaviolin production was doubled by expression of acetyl-CoA carboxylase (ACCase) and 4-fold by combined expression of ACCase, metK1-sp and afsR-sp. Thus, the newly developed Streptomyces venezuelae YJ028 hosts produce heterologous polyketides more efficiently than the parent strain.
AB - Using metabolic engineering, we developed Streptomyces venezuelae YJ028 as an efficient heterologous host to increase the malonyl-CoA pool to be directed towards enhanced production of various polyketides. To probe the applicability of newly developed hosts in the heterologous production of polyketides, we expressed type III polyketide synthase, 1,3,6,8-tetrahydroxynaphthalene synthase, in these hosts. Flaviolin production was doubled by expression of acetyl-CoA carboxylase (ACCase) and 4-fold by combined expression of ACCase, metK1-sp and afsR-sp. Thus, the newly developed Streptomyces venezuelae YJ028 hosts produce heterologous polyketides more efficiently than the parent strain.
KW - 1,3,6,8-tetrahydroxynaphthalene synthase
KW - Acetyl-CoA carboxylase
KW - Flaviolin
KW - Streptomyces venezuelae
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U2 - 10.1007/s10529-009-0152-9
DO - 10.1007/s10529-009-0152-9
M3 - Article
C2 - 19838628
AN - SCOPUS:76449088301
SN - 0141-5492
VL - 32
SP - 277
EP - 282
JO - Biotechnology letters
JF - Biotechnology letters
IS - 2
ER -