Metabolic engineering of Streptomyces venezuelae for malonyl-CoA biosynthesis to enhance heterologous production of polyketides

Sushila Maharjan; Je Won Park; Yeo Joon Yoon; Hei Chan Lee; Jae Kyung Sohng

doi:10.1007/s10529-009-0152-9

Metabolic engineering of Streptomyces venezuelae for malonyl-CoA biosynthesis to enhance heterologous production of polyketides

Sushila Maharjan, Je Won Park, Yeo Joon Yoon, Hei Chan Lee, Jae Kyung Sohng

Research output: Contribution to journal › Article › peer-review

27 Citations (Scopus)

Abstract

Using metabolic engineering, we developed Streptomyces venezuelae YJ028 as an efficient heterologous host to increase the malonyl-CoA pool to be directed towards enhanced production of various polyketides. To probe the applicability of newly developed hosts in the heterologous production of polyketides, we expressed type III polyketide synthase, 1,3,6,8-tetrahydroxynaphthalene synthase, in these hosts. Flaviolin production was doubled by expression of acetyl-CoA carboxylase (ACCase) and 4-fold by combined expression of ACCase, metK1-sp and afsR-sp. Thus, the newly developed Streptomyces venezuelae YJ028 hosts produce heterologous polyketides more efficiently than the parent strain.

Original language	English
Pages (from-to)	277-282
Number of pages	6
Journal	Biotechnology letters
Volume	32
Issue number	2
DOIs	https://doi.org/10.1007/s10529-009-0152-9
Publication status	Published - 2010 Jan
Externally published	Yes

Bibliographical note

Funding Information:
Acknowledgments This work was supported by Agenda program (No. 200905FP014), Rural Development Administration, Republic of Korea and Korean Research Foundation (KRF) by providing research scholarship for a PhD scholar.

Copyright:
Copyright 2010 Elsevier B.V., All rights reserved.

Keywords

1,3,6,8-tetrahydroxynaphthalene synthase
Acetyl-CoA carboxylase
Flaviolin
Streptomyces venezuelae

ASJC Scopus subject areas

Biotechnology
Bioengineering
Applied Microbiology and Biotechnology

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10.1007/s10529-009-0152-9

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abstract = "Using metabolic engineering, we developed Streptomyces venezuelae YJ028 as an efficient heterologous host to increase the malonyl-CoA pool to be directed towards enhanced production of various polyketides. To probe the applicability of newly developed hosts in the heterologous production of polyketides, we expressed type III polyketide synthase, 1,3,6,8-tetrahydroxynaphthalene synthase, in these hosts. Flaviolin production was doubled by expression of acetyl-CoA carboxylase (ACCase) and 4-fold by combined expression of ACCase, metK1-sp and afsR-sp. Thus, the newly developed Streptomyces venezuelae YJ028 hosts produce heterologous polyketides more efficiently than the parent strain.",

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author = "Sushila Maharjan and Park, {Je Won} and Yoon, {Yeo Joon} and Lee, {Hei Chan} and Sohng, {Jae Kyung}",

note = "Funding Information: Acknowledgments This work was supported by Agenda program (No. 200905FP014), Rural Development Administration, Republic of Korea and Korean Research Foundation (KRF) by providing research scholarship for a PhD scholar. Copyright: Copyright 2010 Elsevier B.V., All rights reserved.",

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doi = "10.1007/s10529-009-0152-9",

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AU - Lee, Hei Chan

AU - Sohng, Jae Kyung

N1 - Funding Information: Acknowledgments This work was supported by Agenda program (No. 200905FP014), Rural Development Administration, Republic of Korea and Korean Research Foundation (KRF) by providing research scholarship for a PhD scholar. Copyright: Copyright 2010 Elsevier B.V., All rights reserved.

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N2 - Using metabolic engineering, we developed Streptomyces venezuelae YJ028 as an efficient heterologous host to increase the malonyl-CoA pool to be directed towards enhanced production of various polyketides. To probe the applicability of newly developed hosts in the heterologous production of polyketides, we expressed type III polyketide synthase, 1,3,6,8-tetrahydroxynaphthalene synthase, in these hosts. Flaviolin production was doubled by expression of acetyl-CoA carboxylase (ACCase) and 4-fold by combined expression of ACCase, metK1-sp and afsR-sp. Thus, the newly developed Streptomyces venezuelae YJ028 hosts produce heterologous polyketides more efficiently than the parent strain.

AB - Using metabolic engineering, we developed Streptomyces venezuelae YJ028 as an efficient heterologous host to increase the malonyl-CoA pool to be directed towards enhanced production of various polyketides. To probe the applicability of newly developed hosts in the heterologous production of polyketides, we expressed type III polyketide synthase, 1,3,6,8-tetrahydroxynaphthalene synthase, in these hosts. Flaviolin production was doubled by expression of acetyl-CoA carboxylase (ACCase) and 4-fold by combined expression of ACCase, metK1-sp and afsR-sp. Thus, the newly developed Streptomyces venezuelae YJ028 hosts produce heterologous polyketides more efficiently than the parent strain.

KW - 1,3,6,8-tetrahydroxynaphthalene synthase

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Metabolic engineering of Streptomyces venezuelae for malonyl-CoA biosynthesis to enhance heterologous production of polyketides

Abstract

Bibliographical note

Keywords

ASJC Scopus subject areas

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