Metabolic rewiring of synthetic pyruvate dehydrogenase bypasses for acetone production in cyanobacteria

Hyun Jeong Lee, Jigyeong Son, Sang Jun Sim, Han Min Woo

Research output: Contribution to journalArticlepeer-review

20 Citations (Scopus)


Designing synthetic pathways for efficient CO2 fixation and conversion is essential for sustainable chemical production. Here we have designed a synthetic acetate-acetyl-CoA/malonyl-CoA (AAM) bypass to overcome an enzymatic activity of pyruvate dehydrogenase complex. This synthetic pathway utilizes acetate assimilation and carbon rearrangements using a methyl malonyl-CoA carboxyltransferase. We demonstrated direct conversion of CO2 into acetyl-CoA-derived acetone as an example in photosynthetic Synechococcus elongatus PCC 7942 by increasing the acetyl-CoA pools. The engineered cyanobacterial strain with the AAM-bypass produced 0.41 g/L of acetone at 0.71 m/day of molar productivity. This work clearly shows that the synthetic pyruvate dehydrogenase bypass (AAM-bypass) is a key factor for the high-level production of an acetyl-CoA-derived chemical in photosynthetic organisms.

Original languageEnglish
Pages (from-to)1860-1868
Number of pages9
JournalPlant Biotechnology Journal
Issue number9
Publication statusPublished - 2020 Sept 1

Bibliographical note

Funding Information:
This work was mainly supported by the Korea CCS R&D Center (KCRC) (2017M1A8A1072034), and the Basic Science Research Program (2017R1A2B2002566) through the National Research Foundation of Korea. H.M.W was supported by the Technology Innovation Program (No. 20000158), funded by the Ministry of Trade, Industry and Energy, Republic of Korea.

Publisher Copyright:
© 2020 The Authors. Plant Biotechnology Journal published by Society for Experimental Biology and The Association of Applied Biologists and John Wiley & Sons Ltd.


  • CO conversion
  • cyanobacteria
  • metabolic engineering
  • synthetic pyruvate dehydrogenase bypass

ASJC Scopus subject areas

  • Biotechnology
  • Agronomy and Crop Science
  • Plant Science


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