Designing synthetic pathways for efficient CO2 fixation and conversion is essential for sustainable chemical production. Here we have designed a synthetic acetate-acetyl-CoA/malonyl-CoA (AAM) bypass to overcome an enzymatic activity of pyruvate dehydrogenase complex. This synthetic pathway utilizes acetate assimilation and carbon rearrangements using a methyl malonyl-CoA carboxyltransferase. We demonstrated direct conversion of CO2 into acetyl-CoA-derived acetone as an example in photosynthetic Synechococcus elongatus PCC 7942 by increasing the acetyl-CoA pools. The engineered cyanobacterial strain with the AAM-bypass produced 0.41 g/L of acetone at 0.71 m/day of molar productivity. This work clearly shows that the synthetic pyruvate dehydrogenase bypass (AAM-bypass) is a key factor for the high-level production of an acetyl-CoA-derived chemical in photosynthetic organisms.
Bibliographical noteFunding Information:
This work was mainly supported by the Korea CCS R&D Center (KCRC) (2017M1A8A1072034), and the Basic Science Research Program (2017R1A2B2002566) through the National Research Foundation of Korea. H.M.W was supported by the Technology Innovation Program (No. 20000158), funded by the Ministry of Trade, Industry and Energy, Republic of Korea.
© 2020 The Authors. Plant Biotechnology Journal published by Society for Experimental Biology and The Association of Applied Biologists and John Wiley & Sons Ltd.
- CO conversion
- metabolic engineering
- synthetic pyruvate dehydrogenase bypass
ASJC Scopus subject areas
- Agronomy and Crop Science
- Plant Science