Metabolically engineered glucose-utilizing Shewanella strains under anaerobic conditions

Donggeon Choi; Sae Bom Lee; Sohyun Kim; Byoungnam Min; In Geol Choi; In Seop Chang

doi:10.1016/j.biortech.2013.12.025

Metabolically engineered glucose-utilizing Shewanella strains under anaerobic conditions

Donggeon Choi, Sae Bom Lee, Sohyun Kim, Byoungnam Min, In Geol Choi, In Seop Chang

Research output: Contribution to journal › Article › peer-review

67 Citations (Scopus)

Abstract

Comparative genome analysis of Shewanella strains predicted that the strains metabolize preferably two- and three-carbon carbohydrates as carbon/electron source because many Shewanella genomes are deficient of the key enzymes in glycolysis (e.g., glucokinase). In addition, all Shewanella genomes are known to have only one set of genes associated with the phosphotransferase system required to uptake sugars. To engineer Shewanella strains that can utilize five- and six-carbon carbohydrates, we constructed glucose-utilizing Shewanella oneidensis MR-1 by introducing the glucose facilitator (glf; ZMO0366) and glucokinase (glk; ZMO0369) genes of Zymomonas mobilis. The engineered MR-1 strain was able to grow on glucose as a sole carbon/electron source under anaerobic conditions. The glucose affinity (Ks) and glucokinase activity in the engineered MR-1 strain were 299.46. mM and 0.259. ±. 0.034. U/g proteins. The engineered strain was successfully applied to a microbial fuel cell system and exhibited current generation using glucose as the electron source.

Original language	English
Pages (from-to)	59-66
Number of pages	8
Journal	Bioresource technology
Volume	154
DOIs	https://doi.org/10.1016/j.biortech.2013.12.025
Publication status	Published - 2014 Feb

Bibliographical note

Funding Information:
We would like to thank Drs. Orianna Bretschger (J. Craig Venter Institute) and Jeffrey Gralnick (University of Minnesota) for the helpful discussions and comments. This research was supported by a grant from the National Research Foundation ( NRF-2013R1A2A2A03014551 ) and by the Development of Biohydrogen Production Technology as part of the Hyperthermophilic Archaea program of the Ministry of Land, Transport, and Maritime Affairs of the Republic of Korea. This work was also supported by the Research Program for Agricultural Science and Technology Development (Project Nos. PJ008517 and PJ008517032013).

Keywords

Anaerobic conditions
Current generation
Glucose utilization
Microbial fuel cells
Shewanella oneidensis MR-1

ASJC Scopus subject areas

Bioengineering
Environmental Engineering
Renewable Energy, Sustainability and the Environment
Waste Management and Disposal

UN SDGs

This output contributes to the following UN Sustainable Development Goals (SDGs)

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10.1016/j.biortech.2013.12.025

Cite this

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title = "Metabolically engineered glucose-utilizing Shewanella strains under anaerobic conditions",

abstract = "Comparative genome analysis of Shewanella strains predicted that the strains metabolize preferably two- and three-carbon carbohydrates as carbon/electron source because many Shewanella genomes are deficient of the key enzymes in glycolysis (e.g., glucokinase). In addition, all Shewanella genomes are known to have only one set of genes associated with the phosphotransferase system required to uptake sugars. To engineer Shewanella strains that can utilize five- and six-carbon carbohydrates, we constructed glucose-utilizing Shewanella oneidensis MR-1 by introducing the glucose facilitator (glf; ZMO0366) and glucokinase (glk; ZMO0369) genes of Zymomonas mobilis. The engineered MR-1 strain was able to grow on glucose as a sole carbon/electron source under anaerobic conditions. The glucose affinity (Ks) and glucokinase activity in the engineered MR-1 strain were 299.46. mM and 0.259. ±. 0.034. U/g proteins. The engineered strain was successfully applied to a microbial fuel cell system and exhibited current generation using glucose as the electron source.",

keywords = "Anaerobic conditions, Current generation, Glucose utilization, Microbial fuel cells, Shewanella oneidensis MR-1",

author = "Donggeon Choi and Lee, {Sae Bom} and Sohyun Kim and Byoungnam Min and Choi, {In Geol} and Chang, {In Seop}",

note = "Funding Information: We would like to thank Drs. Orianna Bretschger (J. Craig Venter Institute) and Jeffrey Gralnick (University of Minnesota) for the helpful discussions and comments. This research was supported by a grant from the National Research Foundation ( NRF-2013R1A2A2A03014551 ) and by the Development of Biohydrogen Production Technology as part of the Hyperthermophilic Archaea program of the Ministry of Land, Transport, and Maritime Affairs of the Republic of Korea. This work was also supported by the Research Program for Agricultural Science and Technology Development (Project Nos. PJ008517 and PJ008517032013).",

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AU - Choi, In Geol

AU - Chang, In Seop

N1 - Funding Information: We would like to thank Drs. Orianna Bretschger (J. Craig Venter Institute) and Jeffrey Gralnick (University of Minnesota) for the helpful discussions and comments. This research was supported by a grant from the National Research Foundation ( NRF-2013R1A2A2A03014551 ) and by the Development of Biohydrogen Production Technology as part of the Hyperthermophilic Archaea program of the Ministry of Land, Transport, and Maritime Affairs of the Republic of Korea. This work was also supported by the Research Program for Agricultural Science and Technology Development (Project Nos. PJ008517 and PJ008517032013).

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N2 - Comparative genome analysis of Shewanella strains predicted that the strains metabolize preferably two- and three-carbon carbohydrates as carbon/electron source because many Shewanella genomes are deficient of the key enzymes in glycolysis (e.g., glucokinase). In addition, all Shewanella genomes are known to have only one set of genes associated with the phosphotransferase system required to uptake sugars. To engineer Shewanella strains that can utilize five- and six-carbon carbohydrates, we constructed glucose-utilizing Shewanella oneidensis MR-1 by introducing the glucose facilitator (glf; ZMO0366) and glucokinase (glk; ZMO0369) genes of Zymomonas mobilis. The engineered MR-1 strain was able to grow on glucose as a sole carbon/electron source under anaerobic conditions. The glucose affinity (Ks) and glucokinase activity in the engineered MR-1 strain were 299.46. mM and 0.259. ±. 0.034. U/g proteins. The engineered strain was successfully applied to a microbial fuel cell system and exhibited current generation using glucose as the electron source.

AB - Comparative genome analysis of Shewanella strains predicted that the strains metabolize preferably two- and three-carbon carbohydrates as carbon/electron source because many Shewanella genomes are deficient of the key enzymes in glycolysis (e.g., glucokinase). In addition, all Shewanella genomes are known to have only one set of genes associated with the phosphotransferase system required to uptake sugars. To engineer Shewanella strains that can utilize five- and six-carbon carbohydrates, we constructed glucose-utilizing Shewanella oneidensis MR-1 by introducing the glucose facilitator (glf; ZMO0366) and glucokinase (glk; ZMO0369) genes of Zymomonas mobilis. The engineered MR-1 strain was able to grow on glucose as a sole carbon/electron source under anaerobic conditions. The glucose affinity (Ks) and glucokinase activity in the engineered MR-1 strain were 299.46. mM and 0.259. ±. 0.034. U/g proteins. The engineered strain was successfully applied to a microbial fuel cell system and exhibited current generation using glucose as the electron source.

KW - Anaerobic conditions

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KW - Glucose utilization

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Metabolically engineered glucose-utilizing Shewanella strains under anaerobic conditions

Abstract

Bibliographical note

Keywords

ASJC Scopus subject areas

UN SDGs

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