Metabolism perturbation Caused by the overexpression of carbon monoxide dehydrogenase/Acetyl-CoA synthase gene complex accelerated gas to acetate conversion rate of Eubacterium limosum KIST612

Hyunsoo Kang; Byeonghyeok Park; Soyoung Oh; Duleepa Pathiraja; Ji Yeon Kim; Seunghyeon Jung; Jiyeong Jeong; Minseok Cha; Zee Yong Park; In Geol Choi; In Seop Chang

doi:10.1016/j.biortech.2021.125879

Metabolism perturbation Caused by the overexpression of carbon monoxide dehydrogenase/Acetyl-CoA synthase gene complex accelerated gas to acetate conversion rate of Eubacterium limosum KIST612

Hyunsoo Kang, Byeonghyeok Park, Soyoung Oh, Duleepa Pathiraja, Ji Yeon Kim, Seunghyeon Jung, Jiyeong Jeong, Minseok Cha, Zee Yong Park, In Geol Choi, In Seop Chang

Research output: Contribution to journal › Article › peer-review

14 Citations (Scopus)

Abstract

Microbial conversion of carbon monoxide (CO) to acetate is a promising upcycling strategy for carbon sequestration. Herein, we demonstrate that CO conversion and acetate production rates of Eubacterium limosum KIST612 strain can be improved by in silico prediction and in vivo assessment. The mimicked CO metabolic model of KIST612 predicted that overexpressing the CO dehydrogenase (CODH) increases CO conversion and acetate production rates. To validate the prediction, we constructed mutant strains overexpressing CODH gene cluster and measured their CO conversion and acetate production rates. A mutant strain (ELM031) co-overexpressing CODH, coenzyme CooC2 and ACS showed a 3.1 × increased specific CO oxidation rate as well as 1.4 × increased specific acetate production rate, compared to the wild type strain. The transcriptional and translational data with redox balance analysis showed that ELM031 has enhanced reducing potential from up-regulation of ferredoxin and related metabolism directly linked to energy conservation.

Original language	English
Article number	125879
Journal	Bioresource technology
Volume	341
DOIs	https://doi.org/10.1016/j.biortech.2021.125879
Publication status	Published - 2021 Dec

Bibliographical note

Funding Information:
This work was supported by grants from the National Research Foundation of Korea (NRF) funded by the Korean Government (2021R1A5A1028138), the C1 Gas Refinery Program (2021M3D3A1A01079730) through the NRF funded by the Ministry of Science and ICT. It was also supported by the New & Renewable Energy Core Technology Program of the Korea Institute of Energy Technology Evaluation and Planning funded by the Ministry of Trade, Industry and Energy (No. 20173010092460). A complete list of abbreviations used for genome-scale modeling is available in the supplementary information .

Publisher Copyright:
© 2021

Keywords

Acetogen
CO dehydrogenase
Eubacterium limosum
Homologous overexpression
Metabolic change

ASJC Scopus subject areas

Bioengineering
Environmental Engineering
Renewable Energy, Sustainability and the Environment
Waste Management and Disposal

UN SDGs

This output contributes to the following UN Sustainable Development Goals (SDGs)

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10.1016/j.biortech.2021.125879

Cite this

Kang, H., Park, B., Oh, S., Pathiraja, D., Kim, J. Y., Jung, S., Jeong, J., Cha, M., Park, Z. Y., Choi, I. G., & Chang, I. S. (2021). Metabolism perturbation Caused by the overexpression of carbon monoxide dehydrogenase/Acetyl-CoA synthase gene complex accelerated gas to acetate conversion rate of Eubacterium limosum KIST612. Bioresource technology, 341, Article 125879. https://doi.org/10.1016/j.biortech.2021.125879

Metabolism perturbation Caused by the overexpression of carbon monoxide dehydrogenase/Acetyl-CoA synthase gene complex accelerated gas to acetate conversion rate of Eubacterium limosum KIST612. / Kang, Hyunsoo; Park, Byeonghyeok; Oh, Soyoung et al.
In: Bioresource technology, Vol. 341, 125879, 12.2021.

Research output: Contribution to journal › Article › peer-review

Kang, H, Park, B, Oh, S, Pathiraja, D, Kim, JY, Jung, S, Jeong, J, Cha, M, Park, ZY, Choi, IG & Chang, IS 2021, 'Metabolism perturbation Caused by the overexpression of carbon monoxide dehydrogenase/Acetyl-CoA synthase gene complex accelerated gas to acetate conversion rate of Eubacterium limosum KIST612', Bioresource technology, vol. 341, 125879. https://doi.org/10.1016/j.biortech.2021.125879

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abstract = "Microbial conversion of carbon monoxide (CO) to acetate is a promising upcycling strategy for carbon sequestration. Herein, we demonstrate that CO conversion and acetate production rates of Eubacterium limosum KIST612 strain can be improved by in silico prediction and in vivo assessment. The mimicked CO metabolic model of KIST612 predicted that overexpressing the CO dehydrogenase (CODH) increases CO conversion and acetate production rates. To validate the prediction, we constructed mutant strains overexpressing CODH gene cluster and measured their CO conversion and acetate production rates. A mutant strain (ELM031) co-overexpressing CODH, coenzyme CooC2 and ACS showed a 3.1 × increased specific CO oxidation rate as well as 1.4 × increased specific acetate production rate, compared to the wild type strain. The transcriptional and translational data with redox balance analysis showed that ELM031 has enhanced reducing potential from up-regulation of ferredoxin and related metabolism directly linked to energy conservation.",

keywords = "Acetogen, CO dehydrogenase, Eubacterium limosum, Homologous overexpression, Metabolic change",

author = "Hyunsoo Kang and Byeonghyeok Park and Soyoung Oh and Duleepa Pathiraja and Kim, {Ji Yeon} and Seunghyeon Jung and Jiyeong Jeong and Minseok Cha and Park, {Zee Yong} and Choi, {In Geol} and Chang, {In Seop}",

note = "Funding Information: This work was supported by grants from the National Research Foundation of Korea (NRF) funded by the Korean Government (2021R1A5A1028138), the C1 Gas Refinery Program (2021M3D3A1A01079730) through the NRF funded by the Ministry of Science and ICT. It was also supported by the New & Renewable Energy Core Technology Program of the Korea Institute of Energy Technology Evaluation and Planning funded by the Ministry of Trade, Industry and Energy (No. 20173010092460). A complete list of abbreviations used for genome-scale modeling is available in the supplementary information . Publisher Copyright: {\textcopyright} 2021",

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T1 - Metabolism perturbation Caused by the overexpression of carbon monoxide dehydrogenase/Acetyl-CoA synthase gene complex accelerated gas to acetate conversion rate of Eubacterium limosum KIST612

AU - Kang, Hyunsoo

AU - Park, Byeonghyeok

AU - Oh, Soyoung

AU - Pathiraja, Duleepa

AU - Kim, Ji Yeon

AU - Jung, Seunghyeon

AU - Jeong, Jiyeong

AU - Cha, Minseok

AU - Park, Zee Yong

AU - Choi, In Geol

AU - Chang, In Seop

N1 - Funding Information: This work was supported by grants from the National Research Foundation of Korea (NRF) funded by the Korean Government (2021R1A5A1028138), the C1 Gas Refinery Program (2021M3D3A1A01079730) through the NRF funded by the Ministry of Science and ICT. It was also supported by the New & Renewable Energy Core Technology Program of the Korea Institute of Energy Technology Evaluation and Planning funded by the Ministry of Trade, Industry and Energy (No. 20173010092460). A complete list of abbreviations used for genome-scale modeling is available in the supplementary information . Publisher Copyright: © 2021

PY - 2021/12

Y1 - 2021/12

N2 - Microbial conversion of carbon monoxide (CO) to acetate is a promising upcycling strategy for carbon sequestration. Herein, we demonstrate that CO conversion and acetate production rates of Eubacterium limosum KIST612 strain can be improved by in silico prediction and in vivo assessment. The mimicked CO metabolic model of KIST612 predicted that overexpressing the CO dehydrogenase (CODH) increases CO conversion and acetate production rates. To validate the prediction, we constructed mutant strains overexpressing CODH gene cluster and measured their CO conversion and acetate production rates. A mutant strain (ELM031) co-overexpressing CODH, coenzyme CooC2 and ACS showed a 3.1 × increased specific CO oxidation rate as well as 1.4 × increased specific acetate production rate, compared to the wild type strain. The transcriptional and translational data with redox balance analysis showed that ELM031 has enhanced reducing potential from up-regulation of ferredoxin and related metabolism directly linked to energy conservation.

AB - Microbial conversion of carbon monoxide (CO) to acetate is a promising upcycling strategy for carbon sequestration. Herein, we demonstrate that CO conversion and acetate production rates of Eubacterium limosum KIST612 strain can be improved by in silico prediction and in vivo assessment. The mimicked CO metabolic model of KIST612 predicted that overexpressing the CO dehydrogenase (CODH) increases CO conversion and acetate production rates. To validate the prediction, we constructed mutant strains overexpressing CODH gene cluster and measured their CO conversion and acetate production rates. A mutant strain (ELM031) co-overexpressing CODH, coenzyme CooC2 and ACS showed a 3.1 × increased specific CO oxidation rate as well as 1.4 × increased specific acetate production rate, compared to the wild type strain. The transcriptional and translational data with redox balance analysis showed that ELM031 has enhanced reducing potential from up-regulation of ferredoxin and related metabolism directly linked to energy conservation.

KW - Acetogen

KW - CO dehydrogenase

KW - Eubacterium limosum

KW - Homologous overexpression

KW - Metabolic change

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Metabolism perturbation Caused by the overexpression of carbon monoxide dehydrogenase/Acetyl-CoA synthase gene complex accelerated gas to acetate conversion rate of Eubacterium limosum KIST612

Abstract

Bibliographical note

Keywords

ASJC Scopus subject areas

UN SDGs

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