Metabolomic differentiation of deer antlers of various origins by 1H NMR spectrometry and principal components analysis

Hyung Kyoon Choi; Kyoung Hee Kim; Kyoung Heon Kim; Young Suk Kim; Min Won Lee; Wan Kyun Whang

doi:10.1016/j.jpba.2006.01.036

Metabolomic differentiation of deer antlers of various origins by ¹H NMR spectrometry and principal components analysis

Hyung Kyoon Choi, Kyoung Hee Kim, Kyoung Heon Kim, Young Suk Kim, Min Won Lee, Wan Kyun Whang

Research output: Contribution to journal › Article › peer-review

18 Citations (Scopus)

Abstract

The metabolomic analysis of various types of deer antler was performed by ¹H NMR spectrometry and principal components analysis (PCA). The PCA of the ¹H NMR spectra of the aqueous fractions allowed a clear discrimination between antler samples according to their origins by the first three principal components (PC1, PC2, and PC3), which cumulatively accounted for 93.5% of the variation in all variables. In particular, the score plots by the combination of PC1 and PC3 allowed an excellent separation of the antler samples. In addition, the major peaks in ¹H NMR spectra contributing to the discrimination were assigned to lactate, alanine, acetic acid, choline, glycine, valine, tyrosine, and phenylalanine. This metabolomic-analysis-based method allows various types of deer antler to be efficiently differentiated without any pre-purification steps.

Original language	English
Pages (from-to)	1047-1050
Number of pages	4
Journal	Journal of Pharmaceutical and Biomedical Analysis
Volume	41
Issue number	3
DOIs	https://doi.org/10.1016/j.jpba.2006.01.036
Publication status	Published - 2006 Jun 7

Keywords

Deer antler
H NMR
Metabolomic analysis
Principal component analysis

ASJC Scopus subject areas

Analytical Chemistry
Pharmaceutical Science
Drug Discovery
Spectroscopy
Clinical Biochemistry

Access to Document

10.1016/j.jpba.2006.01.036

Cite this

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title = "Metabolomic differentiation of deer antlers of various origins by 1H NMR spectrometry and principal components analysis",

abstract = "The metabolomic analysis of various types of deer antler was performed by 1H NMR spectrometry and principal components analysis (PCA). The PCA of the 1H NMR spectra of the aqueous fractions allowed a clear discrimination between antler samples according to their origins by the first three principal components (PC1, PC2, and PC3), which cumulatively accounted for 93.5% of the variation in all variables. In particular, the score plots by the combination of PC1 and PC3 allowed an excellent separation of the antler samples. In addition, the major peaks in 1H NMR spectra contributing to the discrimination were assigned to lactate, alanine, acetic acid, choline, glycine, valine, tyrosine, and phenylalanine. This metabolomic-analysis-based method allows various types of deer antler to be efficiently differentiated without any pre-purification steps.",

keywords = "Deer antler, H NMR, Metabolomic analysis, Principal component analysis",

author = "Choi, {Hyung Kyoon} and Kim, {Kyoung Hee} and Kim, {Kyoung Heon} and Kim, {Young Suk} and Lee, {Min Won} and Whang, {Wan Kyun}",

year = "2006",

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AU - Choi, Hyung Kyoon

AU - Kim, Kyoung Hee

AU - Kim, Kyoung Heon

AU - Kim, Young Suk

AU - Lee, Min Won

AU - Whang, Wan Kyun

PY - 2006/6/7

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AB - The metabolomic analysis of various types of deer antler was performed by 1H NMR spectrometry and principal components analysis (PCA). The PCA of the 1H NMR spectra of the aqueous fractions allowed a clear discrimination between antler samples according to their origins by the first three principal components (PC1, PC2, and PC3), which cumulatively accounted for 93.5% of the variation in all variables. In particular, the score plots by the combination of PC1 and PC3 allowed an excellent separation of the antler samples. In addition, the major peaks in 1H NMR spectra contributing to the discrimination were assigned to lactate, alanine, acetic acid, choline, glycine, valine, tyrosine, and phenylalanine. This metabolomic-analysis-based method allows various types of deer antler to be efficiently differentiated without any pre-purification steps.

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