Barcode nanowires (BNWs) composed of multiple layered segments of different materials are attractive to bioengineering field due to their characteristics that allow the adjustment of physicochemical properties and conjugation with two or more types of biomolecules to facilitate multiple tasks. Here, we report a metallic Fe (iron)-Au (gold) BNW-based platform for capturing CD8 T cells and the interferon-γ(γ) they secrete, both of which play key roles in controlling infectious diseases such as tuberculosis, at the single-cell level. We also describe an efficient approach for conjugating distinct antibodies, which recognize different epitopes to appropriate materials. The platform achieved detection even with 4.45-35.6 μg mL-1 of BNWs. The T cell capture efficiency was close to 100% and the detection limit for interferon-γwas 460 pg mL-1. This work presents a potential guideline for the design of single-cell immunoassay platforms for eliminating diagnostic errors by unambiguously identifying disease-relevant immune mediators.
Bibliographical noteFunding Information:
This work was supported in part by the National Research Foundation of Korea (2015R1A2A1A15053002 and 2014M3A7B4052192), funded by the Ministry of Science, ICT and Future Planning. H.-R.K. acknowledges the financial support provided by the Creative-Pioneering Researchers Program through Seoul National University. Y.S.J. acknowledges the financial support provided by the Global Ph.D. Fellowship Program through the National Research Foundation of Korea (NRF-2015H1A2A1034498), funded by the Ministry of Education.
© 2019 American Chemical Society.
- Fe-Au barcode nanowire
- T cell capture
- single-cell immunoassay
ASJC Scopus subject areas
- General Materials Science